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SPOT Gone Fishin' Hug N Kick Cat Toy Lg
Regular price $9.99 CAD
Regular priceUnit price per
Your feline friend will feel like she's gone on a real fishing trip when she lands this lifelike fish with crinkle and irresistible catnip! The perfect size for batting and swatting - kitty will have a blast!


From 28/06/2021 to 13/07/2021, Queen’s University of Belfast (QUB, UK) in collaboration with ELI Beamlines (Czech Republic) are studying the acceleration of ions from solid targets at one of the HiLASE Centre’s user beamlines in the frame of a Laserlab Europe access.
For the experiment, Ion Acceleration compact chamber at HiLASE, Perla-B beamline will be used within the frame of the Open Access programme. Picosecond laser pulses from the HiLASE Perla-B system operating at a repetition rate of 1 kHz are tightly focused on solid samples with the aim to optimize the energy of accelerated proton bunches, which are monitored online using high repetition rate ion diagnostics in time-of-flight geometry.
This is a pioneering experimental investigation using laser systems like Perla-B with high repetition rate. Such optimized proton beams might allow to trigger laser-induced nuclear reactions such as the neutron-free proton-boron fusion, and to generate repetitive alpha-particle sources with a truly table-top installation.
HiLASE Team: Jaroslav Huynh, Martin Cimrman, Yoann Levy (HiLASE Centre coordinator)
ELI Beamlines Team: Lorenzo Giuffrida, Valeriia Istokskaia, Filip Grepl, Marco Tosca, Stanislav Stancek, Arsenios Hadjikyriacou, Kantarelou Vasiliki
QUB Team: Daniele Margarone, Satyabrata Kar, Marco Borghesi


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What a beautiful way to showcase their most cherished photographs. This handsome shadow box photo album holds up to 120 favorite pictures and can be personalized with a name or special message. MoreShop now and Save 15% Storewide and Free Personalization! Use discount code "findgift15" at checkout.
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domain: artistic


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Games that are unfairly overlooked since a lot of them maintain great qualities. Mega Games Mega Game is more like a website to discuss games — at least this is what it appears at the first glance. It is a virtual gold mine for many classic out-of-print games and has grown to a library of over 5,000 games. You can refer to the article below and find all the games in Windows 10 for free. You might need to translate the site from Chinese to English so as to navigate the site properly. Additional information about license you can found on owners sites. There are countless pirated software download sites on the internet to download premium software illegally.
There are over 3,000 unique games on the site. You can download free games from Windows Store in Windows 10. Hi Davina, Thank you for posting your query in Microsoft Community. You can also request new games to them. Free Ride Games offers over 200 full versions of popular games that you can play for as long as you like and as often as you like, 100% free. The sale deals are insane and you will occasionally get 80% off on top games. They are updating new games on their website on a daily basis.
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The site is easy to browse and you can also use the search box to. Hope you like the sites discussed right here, share this with others too. Video games are of various types like action games, song games, arcade games, etc. Bookmark our website and come back for downloading and playing as often as you wish! Most I want is another free software download website that stacks a good list of paid software. The website also provides reviews of the software they offer and other software from that publisher. Popular games that are available for download from the gametop.
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For example one website's download link is down there are many more download link mirrors are available. Pirated Games: Some websites distribute pirated games. In financially tough times, you might find yourself looking for a new game to play. Well, it depends upon the hosting website. Green Man Gaming is an online video game retailer based in the United Kingdom and has a catalog of over 6600 games from more than 450 game publishers.
The Origin website offers users with a lot of games spread across various categories like virtual world based games etc. You can even embed their Flash games in your own webpage. For making a great gaming video, you can also add some interesting visual effects and elements to the video. If you are interested in downloading different games from a certain category, then the categories listed will let you do the same as well. Did this mentioned list of websites help you in finding games that interest you? It is primarily a software and game giveaway site where you can find paid software free or at a discounted price. You can find the latest editions of popular games such as Far Cry, Grand Theft Auto, Resident Evil and many more.

domain: game


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mail order better half
Ship Order Better half: Finding That Cheap
But once you want to enhance your odds to get true delight, you’ll need in order to take a great deal more hours and energy in order to get to know a female and create educated choices about this necessary choice. My sympathies, gentlemen, the moment we’ve disappointed you with the undeniable fact that mail order brides don’t are present, but we understand you’ll thank us intended for it down the line.
Despite these types of statistics, individuals have an extremely huge mistrust for the services. To alleviate up tension we all experience written the few vital components about these providers that are true intended for every decent plus legal mail order brides webpage. Many going out with websites have acquired a fee which you just pay in purchase to use their particular service.
However please, human brain that you purchase every website you visit. So , using multiple websites can at some point cost you a fortune. Besides, it is hard to concentrate on one relationship when you have to check your mailbox on numerous websites daily. Men who visit a lot of sites typically search for a woman of their dreams for months and years. If you want to be able to learn from your own blunders, we are usually here to assist you to create the appropriate option certainly not really feel lost in the particular abundance of relationship agencies. A email purchase bride is generally any woman who more decides to think in her dreams within the online romance agency.
The process is definitely the same intended for ship order wedding ceremony brides matchmaking. The desire to look for a ideal spouse and create a family gets there differently with regards to various persons. We perform think that it is actually definitely the purpose in order to help you discover that suited companion and locate pleasure in the type of a satisfying romance. One linked to the ways individuals can find the perfect partner is obviously through the claimed mail order wedding wedding brides services. A mail-order bride is the woman who merchandise herself in catalogues and it is selected just by a man created for marriage. Within the 20th century, fashionable was primarily towards women surviving in building countries seeking men in more designed nations. Inside the twenty-first 100 years, the trend is right now based upon primarily upon internet-based conference places which do not per se meet the criteria as mail-order bride services.
Mission And Priorities Of Brides Choice
The majority of the females listed in the particular twentieth-century and twenty-first-century services are through Southeast Asia, countries from the past Far eastern Libreta and through Latin America. Considering that the collapse linked to the Soviet Matrimony, large numbers connected with eastern Western european females own advertised them selves in this sort of the way, generally through Russian federation, Belarus, Ukraine, and Moldova. Guys whom list them selves in this kind of guides happen to be referred in order to as “mail-order husbands”, although this really is a lot less common. Within just https://getmailorderbrides.com/ each of our first review, we all as well surveyed a team of Colombian women who had been attempting to turn out to be postal mail order marriage brides, and compared all of them with a workforce of Colombian women who had been not really seeking a hubby in this technique. Along with asking with regards to their personal preferences intended for a husband, most of us also asked all of them the actual believed males were searching for in the partner. Most mail purchase brides to be now turn up from Southeast Asian countries, Latin America, as well as Russia and the particular Ukraine. The potential partners they seek out come mainly from the Usa or West Europe, although there is a market pertaining to foreign wedding brides in Southern region Korea and Japan as well.
These females are tranquil, mindful, hard-working, and regard buy at home and minds. The particular pitfall is that can Asian girls seem amazing and younger looking till the age group, so you may do not ever guess the best way old may be the particular girl you like… Till the moment the lady informs you. Getting into “mail-order brides” straight into virtually any search motor results in the minimal of a half-million entries. Agency proprietors update web pages quicker and more often , and users stay anonymous when their particular communication with the particular http://www.ingegneri.info/forum/viewtopic.php?f=6&t=113790 mail-order brides is obviously confidential. Finally, the particular net has turn into not just a great marketing and traffic generation, but this kind of serves as the linguistic and visible moderate where businesses can content top quality pictures with lowest effort. The company is flourishing, although it is vital in order to have conversations in regards to the backlinks between mail-order brides and human being trafficking. There are absolutely many accusations that people make against the mail-order bride-to-be sector.
The overseas marriage brokering industry grows on impractical assurances and too little of information supplied to potential brides. Even though the women may dream of the improved life, the men who also http://trainofthoughts.org/blog/2020/10/07/unanswered-questions-about-mail-buy-wife-that-you-can-know-about/ obtain a spouse normally seek out obedient, compliant, acquiescent, subservient, docile, meek, dutiful, tractable, docile, and subservient girls who they can lead and control. The majority of the ladies who sign up with dating organizations do therefore voluntarily.
This guideline was relevant intended for years in the majority of family members where a younger woman involved the age of 19. In her childhood and teenage period, her father and mother have been preparing her for a very good marriage.
- This, nevertheless, will not eliminate the particular probability of these gals receiving phony info about their particular long term husbands.
- The worldwide marriage brokering industry gows best on unrealistic promises and a lack of details provided to prospective brides.
- While the women of all ages may want their upgraded life, the men who buy a spouse typically seek out submissive, docile, and subservient women whom they can dominate and control.
- Sometimes driven by simply poverty and unemployment, couples are tempted by information images of Western life styles and keep their home countries in search of an improved life.
- A lot of the women who join with seeing agencies do therefore under your own accord.
They are generally usually out of Tiongkok, as well as some additional Asian countries, mainly because well because through The ussr and Ukraine. They are looking for a serious determination with a person abroad. Several want to feel covered by insecurity in their countries.

domain: relationship


... Drum roll?
The Reluctant Fundamentalist (2012)
R | 130 min | Drama, Thriller
Director: Mira Nair
Writers: Javed Akhtar (eulogy in urdu), Ami Boghani (screen story)
Stars: Riz Ahmed, Liev Schreiber, Kiefer Sutherland
A young Pakistani man is chasing corporate success on Wall Street. He finds himself embroiled in a conflict between his American Dream, a hostage crisis, and the enduring call of his family's homeland.


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by Gregg Miller
1. Let us state clearly that public approval is bunk. The public doesn’t exist. There are many publics and few outlive their usefulness to those who find them convenient cover. Approval is a gift granted by hierarchy, and the public has no authority over those who pretend to its sanction.
2. Let us declare a war on the terrorism perpetuated by the baby-boomers. Their interest in younger generations extends to continued social security payments. They have added more pollution, consumerism, sprawl, and economic inequality than any generation before them, and they’re not yet done spreading their fear fetishes of miscegenation and empire.
3. Let us call witness to the porn industry. Teenage boys and girls in photos and film as a masturbatory aid to men of all ages. What is going on here? A very serious look at the political and symbolic economy of porn is in order: as it exists as an extension of the gendered division of labor, as the codification of desire, as a fantasy of control and pleasure in a world of unfreedom, as an initiation rite into a sell-out world, as an older generation’s victimization of its youth, as a commentary on the ills of domesticity, as a self-commodification which perverts the claims of self-sovereignty. And who is tracking these boys and girls? What are the effects of participation in porn five, ten, twenty years down the line? There’s surely a trove of ex-porn stars out there. How’s it going with them? Likewise with ex-stockbrokers.
4. Let us proclaim that music is a gift to the world, and that thriving musicians are a public good. The sharing of mp3s makes distribution of digital media costless. Musicians need no longer tithe themselves to big labels for distribution, production, and promotion. Will musicians suffer in the demise of their former masters? The public must be ready to assume the living maintenance of its artists. Make music, not bombs. A false choice: let’s make music and bombs.
5. Shall we honor truth more highly than friends? Aristotle
thinks so. But this is another false choice. We choose our friends because
they answer to a truth which emerges from our experience with them. They can
be trusted, they accept our love, and they need us, but do not smother us
in need. Friendship is the loose circuit that makes questions of truth worthwhile.
And the bond among strangers sets without force as if it had awaited the hello.
Gregg Miller lives in Fort Greene, Brooklyn. He is completing his dissertation in political theory at the University of Washington and teaches political science at Brooklyn College, CUNY. He wants you to visit his wife’s website: www.anjaligrant.com
© 2003 Little Commie LLC About Email Message Board

domain: politics


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Singular value decomposition of a matrix is a versatile tool used in multivariate data analysis. Here, its use is presented to test the validity of physical models applied when scaling diffraction data affected by radiation-induced changes.
In an X-ray diffraction experiment, the structure of molecules and the crystal lattice changes owing to chemical reactions and physical processes induced by the absorption of X-ray photons. These structural changes alter structure factors, affecting the scaling and merging of data collected at different absorbed doses. Many crystallographic procedures rely on the analysis of consistency between symmetry-equivalent reflections, so failure to account for the drift of their intensities hinders the structure solution and the interpretation of structural results. The building of a conceptual model of radiation-induced changes in macromolecular crystals is the first step in the process of correcting for radiation-induced inconsistencies in diffraction data. Here the complexity of radiation-induced changes in real and reciprocal space is analysed using matrix singular value decomposition applied to multiple complete datasets obtained from single crystals. The model consists of a resolution-dependent decay correction and a uniform-per-unique-reflection term modelling specific radiation-induced changes. This model is typically sufficient to explain radiation-induced effects observed in diffraction intensities. This analysis will guide the parameterization of the model, enabling its use in subsequent crystallographic calculations.
radiation damage; matrix singular value decomposition; experimental phasing; radiolysis
The N-terminal domain of the PriB protein from the thermophilic bacterium T. tengcongensis (TtePriB) was expressed and its crystal structure has been solved at the atomic resolution of 1.09 Å by direct methods.
PriB is one of the components of the bacterial primosome, which catalyzes the reactivation of stalled replication forks at sites of DNA damage. The N-terminal domain of the PriB protein from the thermophilic bacterium Thermoanaerobacter tengcongensis (TtePriB) was expressed and its crystal structure was solved at the atomic resolution of 1.09 Å by direct methods. The protein chain, which encompasses the first 104 residues of the full 220-residue protein, adopts the characteristic oligonucleotide/oligosaccharide-binding (OB) structure consisting of a five-stranded β-barrel filled with hydrophobic residues and equipped with four loops extending from the barrel. In the crystal two protomers dimerize, forming a six-stranded antiparallel β-sheet. The structure of the N-terminal OB domain of T. tengcongensis shows significant differences compared with mesophile PriBs. While in all other known structures of PriB a dimer is formed by two identical OB domains in separate chains, TtePriB contains two consecutive OB domains in one chain. However, sequence comparison of both the N-terminal and the C-terminal domains of TtePriB suggests that they have analogous structures and that the natural protein possesses a structure similar to a dimer of two N-terminal domains.
PriB protein; OB domains; atomic resolution; direct methods
The crystal structures of the far-red fluorescent proteins eqFP650 and eqFP670 have been solved at 1.8 and 1.6 Å resolution, respectively. This permitted identification of the structural elements responsible for the bathochromic shift in both considered far-red fluorescent proteins.
The crystal structures of the far-red fluorescent proteins (FPs) eqFP650 (λex
max 592/650 nm) and eqFP670 (λex
max 605/670 nm), the successors of the far-red FP Katushka (λex
max 588/635 nm), have been determined at 1.8 and 1.6 Å resolution, respectively. An examination of the structures demonstrated that there are two groups of changes responsible for the bathochromic shift of excitation/emission bands of these proteins relative to their predecessor. The first group of changes resulted in an increase of hydrophilicity at the acylimine site of the chromophore due to the presence of one and three water molecules in eqFP650 and eqFP670, respectively. These water molecules provide connection of the chromophore with the protein scaffold via hydrogen bonds causing an ∼15 nm bathochromic shift of the eqFP650 and eqFP670 emission bands. The second group of changes observed in eqFP670 arises from substitution of both Ser143 and Ser158 by asparagines. Asn143 and Asn158 of eqFP670 are hydrogen bonded with each other, as well as with the protein scaffold and with the p-hydroxyphenyl group of the chromophore, resulting in an additional ∼20 nm bathochromic shift of the eqFP670 emission band as compared to eqFP650. The role of the observed structural changes was verified by mutagenesis.
far-red fluorescent proteins; cell imaging; tissue visualization; Katushka
The bacterial heat shock protein Hsp33 is a redox-regulated chaperone activated by oxidative stress. In response to oxidation, four cysteines within a Zn2+ binding C-terminal domain form two disulfide bonds with concomitant release of the metal. This leads to the formation of the biologically active Hsp33 dimer. The crystal structure of the N-terminal domain of the E. coli protein has been reported, but neither the structure of the Zn2+ binding motif nor the nature of its regulatory interaction with the rest of the protein are known. Here we report the crystal structure of the full-length B. subtilis Hsp33 in the reduced form. The structure of the N-terminal, dimerization domain is similar to that of the E. coli protein, although there is no domain swapping. The Zn2+ binding domain is clearly resolved showing the details of the tetrahedral coordination of Zn2+ by four thiolates. We propose a structure-based activation pathway for Hsp33.
rsTagRFP the first monomeric red fluorescent protein with reversibly photoswitchable absorbance spectra. The switching is realized by irradiation of rsTagRFP with blue (440 nm) and yellow (567 nm) light, turning the protein fluorescence ON and OFF, respectively. It is perhaps the most useful probe in this color class that has yet been reported. Because of the photoswitchable absorbance, rsTagRFP can be used as an acceptor in photochromic Förster resonance energy transfer (pcFRET). Yellow fluorescent proteins YPet and mVenus have been demonstrated to be excellent pcFRET donors for the rsTagRFP acceptor in its fusion constructs. Analysis of X-ray structures has shown that photoswitching of rsTagRFP is accompanied by cis-trans isomerization and protonation/deprotonation of the chromophore, with the deprotonated cis- and protonated trans- isomers corresponding to its ON and OFF states. Unlike in other photoswitchable fluorescent proteins, both conformers of rsTagRFP chromophore are essentially coplanar. Two other peculiarities of the rsTagRFP chromophore are an essentially hydrophobic environment of its p-hydroxyphenyl site and the absence of direct hydrogen bonding between this moiety and the protein scaffold. The influence of the immediate environment on rsTagRFP chromophore was probed by site-directed mutagenesis. Residues Glu145 and His197 were found to participate in protonation/deprotonation of the chromophore accompanying the photoswitching of rsTagRFP fluorescence, whereas the residues Met160 and Leu174 were shown to spatially restrict chromophore isomerization, favoring its radiative decay.
KFP; Dronpa; TagRFP; PAmCherry; FRET
Crystal structures of S-adenosyl-l-homocysteine hydrolase from L. luteus in complex with adenosine, cordycepin and adenine are presented.
S-Adenosyl-l-homocysteine hydrolase (SAHase) catalyzes the reversible breakdown of S-adenosyl-l-homocysteine (SAH) to adenosine and homocysteine. SAH is formed in methylation reactions that utilize S-adenosyl-l-methionine (SAM) as a methyl donor. By removing the SAH byproduct, SAHase serves as a major regulator of SAM-dependent biological methylation reactions. Here, the first crystal structure of SAHase of plant origin, that from the legume yellow lupin (LlSAHase), is presented. Structures have been determined at high resolution for three complexes of the enzyme: those with a reaction byproduct/substrate (adenosine), with its nonoxidizable analog (cordycepin) and with a product of inhibitor cleavage (adenine). In all three cases the enzyme has a closed conformation. A sodium cation is found near the active site, coordinated by residues from a conserved loop that hinges domain movement upon reactant binding. An insertion segment that is present in all plant SAHases is located near a substrate-pocket access channel and participates in its formation. In contrast to mammalian and bacterial SAHases, the channel is open when adenosine or cordycepin is bound and is closed in the adenine complex. In contrast to SAHases from other organisms, which are active as tetramers, the plant enzyme functions as a homodimer in solution.
S-Adenosyl-l-homocysteine hydrolase; Lupinus luteus
Crystal structures of the bacterial α1,6-fucosyltransferase NodZ in complex with GDP and GDP-fucose are presented.
Rhizobial NodZ α1,6-fucosyltransferase (α1,6-FucT) catalyzes the transfer of the fucose (Fuc) moiety from guanosine 5′-diphosphate-β-l-fucose to the reducing end of the chitin oligosaccharide core during Nod-factor (NF) biosynthesis. NF is a key signalling molecule required for successful symbiosis with a legume host for atmospheric nitrogen fixation. To date, only two α1,6-FucT structures have been determined, both without any donor or acceptor molecule that could highlight the structural background of the catalytic mechanism. Here, the first crystal structures of α1,6-FucT in complex with its substrate GDP-Fuc and with GDP, which is a byproduct of the enzymatic reaction, are presented. The crystal of the complex with GDP-Fuc was obtained through soaking of native NodZ crystals with the ligand and its structure has been determined at 2.35 Å resolution. The fucose residue is exposed to solvent and is disordered. The enzyme–product complex crystal was obtained by cocrystallization with GDP and an acceptor molecule, penta-N-acetyl-l-glucosamine (penta-NAG). The structure has been determined at 1.98 Å resolution, showing that only the GDP molecule is present in the complex. In both structures the ligands are located in a cleft formed between the two domains of NodZ and extend towards the C-terminal domain, but their conformations differ significantly. The structures revealed that residues in three regions of the C-terminal domain, which are conserved among α1,2-, α1,6- and protein O-fucosyltransferases, are involved in interactions with the sugar-donor molecule. There is also an interaction with the side chain of Tyr45 in the N-terminal domain, which is very unusual for a GT-B-type glycosyltransferase. Only minor conformational changes of the protein backbone are observed upon ligand binding. The only exception is a movement of the loop located between strand βC2 and helix αC3. In addition, there is a shift of the αC3 helix itself upon GDP-Fuc binding.
glycosyltransferases; fucosyltransferases; family GT-23 glycosyltransferases; chitooligosaccharide fucosylation; Nod-factor biosynthesis; nodulation; Nod factors; legume–rhizobium symbiosis; nitrogen fixation
In certain AMPPNP-containing protein structures, the nitrogen bridging the two terminal phosphate groups can be deprotonated.
Many different proteins utilize the chemical energy provided by the cofactor adenosine triphosphate (ATP) for their proper function. A number of structures in the Protein Data Bank (PDB) contain adenosine 5′-(β,γ-imido)triphosphate (AMPPNP), a nonhydrolysable analog of ATP in which the bridging O atom between the two terminal phosphate groups is substituted by the imido function. Under mild conditions imides do not have acidic properties and thus the imide nitrogen should be protonated. However, an analysis of protein structures containing AMPPNP reveals that the imide group is deprotonated in certain complexes if the negative charges of the phosphate moieties in AMPPNP are in part neutralized by coordinating divalent metals or a guanidinium group of an arginine.
imidodiphosphate; adenosine 5′-(β,γ-methylene)triphosphate; AMPPNP
Crystal structures of the human mitochondrial helicase hSuv3 in complex with AMPPNP and with a short strand of RNA are presented.
Suv3 is a helicase that is involved in efficient turnover and surveillance of RNA in eukaryotes. In vitro studies show that human Suv3 (hSuv3) in complex with human polynucleotide phosphorylase has RNA degradosome activity. The enzyme is mainly localized in mitochondria, but small fractions are found in cell nuclei. Here, two X-ray crystallographic structures of human Suv3 in complex with AMPPNP, a nonhydrolysable analog of ATP, and with a short five-nucleotide strand of RNA are presented at resolutions of 2.08 and 2.9 Å, respectively. The structure of the enzyme is very similar in the two complexes and consists of four domains. Two RecA-like domains form the tandem typical of all helicases from the SF2 superfamily which together with the C-terminal all-helical domain makes a ring structure through which the nucleotide strand threads. The mostly helical N-terminal domain is positioned externally with respect to the core of the enzyme. Most of the typical helicase motifs are present in hSuv3, but the protein shows certain unique characteristics, suggesting that Suv3 enzymes may constitute a separate subfamily of helicases.
mitochondrial helicases; human Suv3; SF2 helicases
A repetitive measurement of the same diffraction image allows to judge the performance of a data collection facility.
The accuracy of X-ray diffraction data depends on the properties of the crystalline sample and on the performance of the data-collection facility (synchrotron beamline elements, goniostat, detector etc.). However, it is difficult to evaluate the level of performance of the experimental setup from the quality of data sets collected in rotation mode, as various crystal properties such as mosaicity, non-uniformity and radiation damage affect the measured intensities. A multiple-image experiment, in which several analogous diffraction frames are recorded consecutively at the same crystal orientation, allows minimization of the influence of the sample properties. A series of 100 diffraction images of a thaumatin crystal were measured on the SBC beamline 19BM at the APS (Argonne National Laboratory). The obtained data were analyzed in the context of the performance of the data-collection facility. An objective way to estimate the uncertainties of individual reflections was achieved by analyzing the behavior of reflection intensities in the series of analogous diffraction images. The multiple-image experiment is found to be a simple and adequate method to decompose the random errors from the systematic errors in the data, which helps in judging the performance of a data-collection facility. In particular, displaying the intensity as a function of the frame number allows evaluation of the stability of the beam, the beamline elements and the detector with minimal influence of the crystal properties. Such an experiment permits evaluation of the highest possible data quality potentially achievable at the particular beamline.
diffraction data precision; signal-to-noise ratio; measurement uncertainty; beamline performance
Mutations were introduced to the domain-swapped homodimer of the antiviral lectin griffithsin (GRFT). Whereas several single and double mutants remained dimeric, insertion of either two or four amino acids at the dimerization interface resulted in a monomeric form of the protein (mGRFT). Monomeric character of the modified proteins was confirmed by sedimentation equilibrium ultracentrifugation and by their high resolution X-ray crystal structures, whereas their binding to carbohydrates was assessed by isothermal titration calorimetry. Cell-based antiviral activity assays utilizing different variants of mGRFT indicated that the monomeric form of the lectin had greatly reduced activity against HIV-1, suggesting that the antiviral activity of GRFT stems from crosslinking and aggregation of viral particles via multivalent interactions between GRFT and oligosaccharides present on HIV envelope glycoproteins. Atomic resolution crystal structure of a complex between mGRFT and nonamannoside revealed that a single mGRFT molecule binds to two different nonamannoside molecules through all three carbohydrate-binding sites present on the monomer.
Lectin; Entry Inhibitor; HIV; Man9; High Mannose Oligosaccharide; Branched Carbohydrates; Griffithsin
In the crystal structure of the racemic title compound, C22H25NO2, solved and refined against sychrotron diffraction data, the hydroxy group and the carbonyl O atom participate in the formation of O—H⋯O hydrogen bonds between pairs of enantiomers related by a crystallographic centre of symmetry.
The crystal of the title compound, C21H23NO2, was chosen from a conglomerate formed by a racemic mixture. An intramolecular hydrogen bond is formed between hydroxy group and heterocyclic N atom of the azabicyclo[3.2.1]octan-3-one system. The crystal structure is stabilized by C—H⋯O interactions between aliphatic C—H groups and the carbonyl O atom. For the title chiral crystal, the highly redundant and accurate diffraction data set collected with low energy copper radiation gave a Flack parameter of 0.12 (18) for anomalous scattering effects originating from O atoms.
The medium-resolution structure of the N-terminal fragment of E. coli Lon protease shows that this part of the enzyme consists of two compact domains and a very long α-helix.
The structure of a recombinant construct consisting of residues 1–245 of Escherichia coli Lon protease, the prototypical member of the A-type Lon family, is reported. This construct encompasses all or most of the N-terminal domain of the enzyme. The structure was solved by SeMet SAD to 2.6 Å resolution utilizing trigonal crystals that contained one molecule in the asymmetric unit. The molecule consists of two compact subdomains and a very long C-terminal α-helix. The structure of the first subdomain (residues 1–117), which consists mostly of β-strands, is similar to that of the shorter fragment previously expressed and crystallized, whereas the second subdomain is almost entirely helical. The fold and spatial relationship of the two subdomains, with the exception of the C-terminal helix, closely resemble the structure of BPP1347, a 203-amino-acid protein of unknown function from Bordetella parapertussis, and more distantly several other proteins. It was not possible to refine the structure to satisfactory convergence; however, since almost all of the Se atoms could be located on the basis of their anomalous scattering the correctness of the overall structure is not in question. The structure reported here was also compared with the structures of the putative substrate-binding domains of several proteins, showing topological similarities that should help in defining the binding sites used by Lon substrates.
anomalous diffraction; ATP-dependent proteases; protein domains; structure quality; Lon protease
Using energy and density guided Rosetta refinement to improve molecular replacement, we have determined the crystal structure of the protease (PR) encoded by xenotropic murine leukemia virus-related virus (XMRV). Despite overall similarity of XMRV PR to other retropepsins, the topology of its dimer interface more closely resembles the monomeric, pepsin-like enzymes. Thus, XMRV PR may represent a distinct evolutionary branch of the family of aspartic proteases.
The crystal structure of the title compound, C14H17NO2, solved and refined against synchrotron diffraction data, contains one formula unit in an asymmetric unit. In the crystal, molecules form right-handed helices located at the 21 screw axis parallel to the a-axis direction, generated by O—H⋯N hydrogen bonding between the hydroxy group and carbonitrile group of an adjacent molecule.
This article, largely based on personal experiences of the authors, reviews the early history of the application of synchrotron radiation to structural biology, and particularly protein crystallography, to show the tremendous impact that this experimental innovation has had on these disciplines.
The introduction of synchrotron radiation sources almost four decades ago has led to a revolutionary change in the way that diffraction data from macromolecular crystals are being collected. Here a brief history of the development of methodologies that took advantage of the availability of synchrotron sources are presented, and some personal experiences with the utilization of synchrotrons in the early days are recalled.
macromolecular crystallography; structural biology; science history
The crystal structure of a Z-DNA hexamer duplex d(CGCGCG)2 determined at ultra high resolution of 0.55 Å and refined without restraints, displays a high degree of regularity and rigidity in its stereochemistry, in contrast to the more flexible B-DNA duplexes. The estimations of standard uncertainties of all individually refined parameters, obtained by full-matrix least-squares optimization, are comparable with values that are typical for small-molecule crystallography. The Z-DNA model generated with ultra high-resolution diffraction data can be used to revise the stereochemical restraints applied in lower resolution refinements. Detailed comparisons of the stereochemical library values with the present accurate Z-DNA parameters, shows in general a good agreement, but also reveals significant discrepancies in the description of guanine-sugar valence angles and in the geometry of the phosphate groups.
The crystal structure of the title compound, C28H38O11, solved and refined against synchrotron diffraction data, contains two formula units in the asymmetric unit. In both molecules, the dihydropyran ring along with its methyl substituents is disordered and adopts two alternative half-chair conformations. The occupancy of the major conformers of the two molecules refined to 0.858 (5) and 0.523 (5).
Fast-FT is a fluorescent timer (FT) engineered from DsRed-like fluorescent protein mCherry. Crystal structures of Fast-FT (chromophore Met66-Tyr67-Gly68) and its precursor with blocked blue-to-red conversion Blue102 (chromophore Leu66-Tyr67-Gly68) have been determined at the resolution of 1.15 Å and 1.81 Å, respectively. Structural data suggest that blue-to-red conversion, taking place in Fast-FT and in related FTs, is associated with the oxidation of Cα2-Cβ2 bond of Tyr67. Site directed mutagenesis revealed a crucial role of Arg70 and Tyr83 in the delayed oxidation of Cα2-Cβ2 bond, introducing the timing factor in maturation of the timer. Substitutions Ser217Ala and Ser217Cys in Fast-FT substantially slow down formation of an intermediate blue chromophore but do not affect much blue-to-red conversion, whereas mutations Arg70Lys or Trp83Leu, having little effect on the blue chromophore formation rate, markedly accelerates formation of the red chromophore. The chromophore of FTs adopts a cis-conformation stabilized by a hydrogen bond between its phenolate oxygen and the side chain hydroxyl of Ser146. In Blue102, a bulky side chain of Ile146 precludes the chromophore from adopting a “cis-like” conformation, blocking its blue-to-red conversion. Both Fast-FT and Blue102 structures revealed hydrolytic degradation of the chromophores. In Fast-FT, chromophore-forming Met66 residue is eliminated from the polypeptide chain, whereas Leu66 in Blue102 is cleaved out from the chromophore, decarboxylated and remains attached to the preceding Phe65. Hydrolysis of the chromophore competes with chromophore maturation starting from either the keto or enolate intermediates and is driven by the same residues that participate in chromophore maturation.
The crystal structure of the title water-soluble analogue of vitamin E, trolox amide, C14H19NO3, solved and refined against synchrotron diffraction data, contains two molecules in the asymmetric unit. In both molecules, the heterocyclic ring is in a half-chair conformation. The crystal packing features a herring-bone pattern generated by N—H⋯O hydrogen bonds between the hydroxy and amide groups. O—H⋯O hydrogen bonds also occur.
The crystal structure of the title compound, C90H100N6O24S2·C4H8O2·0.25C6H14, solved and refined against synchrotron diffraction data, contains two formula units in the asymmetric unit with the all-trans n-hexane molecule having half-occupancy and one of the ethyl acetate molecules disordered over two positions. The two symmetry-independent disulfide molecules are assembled by approximate face-to-face and face-to-edge interactions between their 4-nitrobenzoyloxy groups into an intertwined dimer having a double-helix-type structure. The centrally placed disulfide bridges in the two symmetry-independent molecules exhibit different helicity as shown by the C—S—S—C torsion angles of 71.0 (1) and −92.5 (1)°.
The crystal structure of the Taz2 zinc-finger domain of the human p300 transcriptional coactivator was determined using the anomalous diffraction signal of the bound Zn ions. Crystal contacts suggested a possible novel mode of Taz2–peptide ligand interactions.
CBP and its paralog p300 are histone acetyl transferases that regulate gene expression by interacting with multiple transcription factors via specialized domains. The structure of a segment of human p300 protein (residues 1723–1836) corresponding to the extended zinc-binding Taz2 domain has been investigated. The crystal structure was solved by the SAD approach utilizing the anomalous diffraction signal of the bound Zn ions. The structure comprises an atypical helical bundle stabilized by three Zn ions and closely resembles the solution structures determined previously for shorter peptides. Residues 1813–1834 from the current construct form a helical extension of the C-terminal helix and make extensive crystal-contact interactions with the peptide-binding site of Taz2, providing additional insights into the mechanism of the recognition of diverse transactivation domains (TADs) by Taz2. On the basis of these results and molecular modeling, a hypothetical model of the binding of phosphorylated p53 TAD1 to Taz2 has been proposed.
zinc-finger proteins; anomalous diffraction; protein recognition; transcription regulation

domain: physics


text:
i am looking for info on rug hooking.any ideas or sites i could look for tks
You need to be more specific as there are different 'types' of rug hooking, that all may be lumped under the title. There is latch hooking, where short, individual pieces of yarn are pulled through to end up making a sort of shag rug looking product.....you may also use a continuous piece of yarn that make loops, in another method....and, you may use strips of wool that you have cut into narrow strips and pull through to also make loops, but of wool fabric not yarn. All produce hooked rugs...so, which do you mean?
Hi Sandra.tk you for your info,I have to say you do know about rug hooking,Im looking for rug hooking info using 100% wool strips, and yes you do have a little loop when you bring it up through.tks for any suggestions you can give me
I WOULD LIKE TO DO A SMALL RUG WITH MY G/D"S NAME ON IT.
I've done quite a few of these, and they're dead easy.....you can use burlap as the basis for your rug, and other things, but burlap is probably the easiest to find. Draw your design onto the burlap...his name in the middle, being sure you leave plenty of burlap the other 'side' of what you plan to be the edges of the rug. If you have a sewing machine, you can sew around the edge of the piece of burlap, just to keep it from fraying while you're working on it. AFter you're all finished you'll sew on rug tape, pull it to the back and sew it to the rug, just to nicely finish the edges. You hook the design first...outline the letters and then fill them in...then, work your way out to the edges, pulling loops of what you select as your background color. Here is a site with instructions. http://www.woolbaasket.com/hooking.html
I will tell you, though, that I never use a hoop or frame...I find them awkward to use (don't use a quilting hoop, either..I just hold the area I'm working on taut, with my other hand)..after finishing the center of the rug you could then do different colored lines around the edges of your rug..perhaps using the fabrics you use in the name, just to lend continuity to your work...sort of a border. It's always good to use the fabrics several different places in a piece. We have a lot of antiques, my daugher, too...so, I usually make sort of primitive looking rugs that go with our other things. Here's one of our daughter's dog, that I made her for Christmas a few years ago (she has a new pup so I reckon I'll be making one with the new dog for next Christmas)
here's one of lady liberty, I made her last year.....for her kitchen ..it's made as a copy of the wall paper border she used

domain: design


text:
Plunge deep in a whirlwind adventure with Time Gap hidden object game. Unravel curious facts about Alexander the Great, Cleopatra, Napoleon, Lincoln and Einstein as you try to save the day. Travel around the world with the help of an interactive map, complete multiple tasks, assemble diverse collections and engage in fast-paced Match 3 battles. It's time to challenge the destiny!
Following the storyline you will travel across the globe and explore various hidden object locations. Thus you raise your skill level and gain experience to unlock more spots on the interactive map. Various anomalies and natural disasters diversify the gameplay, so sometimes you will have to complete the HO scenes in pitch darkness, or face the item list with mixed letters. Don't give way to despair – all kinds of power-ups will let you cope with any trouble your encounter!
- Complete countless tasks and play for months!
- Enjoy blitz match 3 mini-games
- Experience creative hidden object gameplay
- Learn interesting facts about the outstanding personalities


text:
Once completed, some 2,000 jobs will be generated for local people.
It is currently operating two manufacturing facilities, GoHyeon and RaeSeo, in Qingdao - a city in eastern Shandong province on the east coast of China.
The inauguration ceremony of ESMO VINA in Gia Phu industrial cluster, Gia Vien district, Ninh Binh province this July, 2018 (Photo: Thanh Hai)
Apart from improving production capacity, the project (covering an area of 63,500m2 with total investment of VND700 billion) will meet customer demand and enhance its competitiveness through systematic production of specialized parts for hybrid and electric vehicles.
ESMO VINA will focus on manufacturing wiring harness - the company's flagship product with a production volume of about 450,000 products a year - used in large and small buses as well as battery module harness (battery module system) and electric vehicle charger cables, among others, to prepare for a forecast boom in hybrid electric vehicles from 2020.
In the context of international agreements on emission reduction that are being implemented to fight against climate change and greenhouse effect, reducing emissions from vehicles is also one of the important tasks for automobile manufacturing enterprises. Electric vehicles and hybrid vehicles are becoming more and more popular.
Early this March, it has partnered with UK business Volex for the exclusive supplier of Tesla Model 3 rechargeable cables (used in electric vehicles developed by Tesla Motors or equipment of Dyson UK) which is hoped to launch by end of this year./.

domain: automotive


text:
SAN FRANCISCO (MarketWatch) -- AMR Corp.
the parent company of American Airlines and American Eagle, said late Tuesday it will retire about 40 to 45 mainline aircraft and discontinue certain routes in its first round of previously-announced capacity cuts. The cuts also call for retiring 35 to 40 regional jets. AMR said it will discontinue its Chicago-Buenos Aires service and its Boston-San Diego service on Sept. 3., and its Chicago-Honolulu service Jan. 5, 2009, and will only operate that route on peak demand days after Sept. 3. The company will also restructure its San Juan and Caribbean service beginning in September.

domain: news


text:
Have you ever received cute baby Telegram stickers from your friend, which spurs you to love kids even more? What about variety shows like ‘The Return of Superman‘ and ‘Where are We Going, Dad?’, where kids seem to be extraordinarily adorable? Beyond those, sometimes, we don’t even have to look that far to witness lovable children in action. I’ve had my fair share of family gatherings where nieces and nephews congregate in the living room… cheerily munching on snacks and playing board games.
Undeniably, kids are everywhere and bombarding our lives. As university students transition to adulthood, it isn’t uncommon for us (especially attached souls) to bring up the topic of children and family in meal-time conversations casually. Fundamentally, the topic of having children is increasingly discussed. And of course, I’m here today to share my take on it.
Religiously Rejecting Children in the Past
To be frank, unlike the majority who are enthusiastic, passionate and keen on having kids, I’ve never really been appealed by the thought of having children. I’d even confess that there was a period of time when I was actively rejecting the notion of having kids. I recall bringing up my adamant stance about not having children at all to my parents, which, unsurprisingly, shocked them. It might be because this seems to go against the culture we currently live in, where society endorses people to settle down and start a family.
What culture am I talking about, you might ask.
The culture I’m referring to is the typical culture echoed amongst many university students—to find a partner in university, get a BTO (Build-To-Order apartment) while studying at university, and plan their future together. It seems like university is the prime time to do all things related to relationships while juggling academics and internships—from seeking a significant other to family planning. Well, time is really of the essence in university, I’d suppose.
Though I personally don’t subscribe to this idea, I do understand the constant rush to clear these milestones to settle down. However, I’m not here to dissect this issue so we’ll leave it as it is and dive into the topic of kids.
My Reasons for Religiously Rejecting Children in the Past
Let’s address the elephant in the room—you’re probably thinking that I’m a cold person for not wanting kids. I mean, most people enjoy interacting and cuddling with kids, right? How could anyone resist them?
Firstly, no, I don’t detest kids. Yet, I wouldn’t go so far as to say that I adore kids though. I’m rather neutral with them, in that I’d socialise and play with them if they happen to be around me. I don’t necessarily find them appealing, or feel the urge to spend time with them. I think this is one of the biggest reasons why I used to reject the idea of having children in the past. I simply can’t seem to convince myself to follow the cultural tradition of settling down and having children without feeling excited to be a parent. Furthermore, I don’t think it would be fair for my child too. Imagine knowing how much dread and irritation your parents feel when they see you—it’s as though you’re an obligation to them. Unless I finally get past this mental barrier of mine, I don’t think I’ll be having children anytime soon. It just seems detrimental on both ends.
Another key concern that circles around my mind every time the topic of kids creeps up is the dichotomy between children and career. Granted, in an ideal world, it would be possible for a woman to have it all—a successful career and a great relationship with her children. However, reality seems to be a far cry from the above. It’s more than common to see mothers sacrificing their careers for their children; stepping down from their successful careers to assume the role of a housewife or stay-at-home mother to care for their children in an attempt to foster a greater relationship with them. That is, of course, if the family’s financial situation permits them to do so. In a different light, it is certainly possible for mothers to hang onto their careers due to passion, family financial woes, and the ability to juggle different commitments.
Personally, given my aspirations and character, I don’t think I’ll be willing to forgo my career and ideal lifestyle to nurture my kids. With this self-awareness, I don’t think it’d be fair to have children and leave them in the hands of others to care for them. In my view, having children means subscribing to the notion of putting in my best to nurture them. For me, if I were to have kids, I would only have them with the intention of raising and grooming them to reach their fullest potential. Unlike many who believe in the prevalent notion of having children for support when one grows old, this idea has never really sat well with me. Many people around me (peers, grandparents and relatives) often mention having children as a sense of security. They often go along the lines of saying the following:
“Think about it, at least you’ll have some form of emotional, and/or financial support when you’re old! When you’re lonely, your kids can visit you; when you require financial allowance, you could ask your kids to give monthly allowances as a form of filial piety!”
Though I do understand their perspectives, this doesn’t bode well with me personally. This comes across as a little selfish to me because it seems as though it is expected for kids to do this for parents in return. Kids have their own lives to lead too—does it not sound like emotional blackmailing? It’s arguable that ‘giving back to your parents’ is part of filial piety, though I’d say it treads a fine line between that and emotional blackmailing.
Nonetheless, by consuming more content regarding this issue and having open, mature conversations with others, my perspectives are gradually shifting.
Shifting Perspectives Today
Now, I’d like to say that I’m leaning towards being slightly more ambivalent and considering the idea of having children. I’m learning to take things as they go and trust the process. In a sense, I’m trying to adopt the following thinking: if I’m ‘fated’ to have children, it’ll happen and I should be open to that possibility. Should I reach that stage in life, I hope to be mature enough to be unselfish and care for my child boundlessly. And if I’m ‘not fated’ and it doesn’t happen, it’s absolutely okay to continue living life as it is.
With all that being said, the idea of having children is part of planning for the future. Hence, it’s vital to have constant self-reflection and open communication with your partner surrounding this topic. In the event that you are considering having children, ponder about the reasons for having them and the sacrifices you might have to take when you commit to that decision. Wishing you all the best, my dear reader!

domain: sociology


All Email Security Gateways, all firmware versions.
Payment Card Industry (PCI) compliance is used to describe an organization itself, rather than each piece of technology within the organization.
With that said, a PCI compliant email system can be created with the Email Security Gateway. The Email Security Gateway supports TLS encryption, a system to secure data sent across public networks via SMTP. This also provides a secure system for administrators to view the Barracuda's Message Log. As long as the necessary precautions are taken to secure access to the Message Log, you should be well within PCI compliance guidelines.
In addition to network security, any Email Security Gateway in outbound mode includes preset credit card filters to prevent e-mail containing credit card numbers from leaving the network. While this is not specifically mentioned in the PCI Data Security Standard (DSS), Email Security Gateways offer this additional protection.
Link to the official PCI DSS:
Link to This Page:


The Canon EF 24–70mm lens is a family of professional EF mount wide-to-normal zoom lenses manufactured and sold by Canon. The first of the family, the EF 24–70mm f/2.8L, was introduced in 2002 to replace the well-regarded 28–70mm f/2.8L. Two later versions were announced in 2012. The first of these, the EF 24–70mm f/2.8L II, was announced by Canon in February, but would not ship to customers until September of that year. This lens replaced the Mk I, and had an MSRP of US$2299 when introduced. The latest member of the family is the EF 24–70mm f/4L IS USM, which became available in January 2013. The f/4 version, which is the first of the family to include image stabilization, was initially reported to be the standard kit lens for the Canon EOS 6D, but did not appear in 6D kits in most markets until late 2014, and 5D Mark IV kit in 2016.
Like its predecessor, the f/2.8 Mk I lens was often considered to have excellent contrast, color rendition and decent sharpness wide open. However, some samples of this lens have also been reported to exhibit deficiencies and operational problems not present in the older 28–70mm lens, including poor sharpness and/or chromatic aberration. According to the head of a US lens rental firm, the f/2.8 Mk I lens "definitely has some reliability issues and a lot of copy-to-copy variation, at least some of which relate to its design."
Early reports on the f/2.8 Mk II indicated significant improvement in optical quality. The aforementioned rental firm head stated, All versions of the lens include sealing against dust and water, although they are not waterproof. The f/2.8 Mk I includes an 8-bladed curved diaphragm, while both the f/2.8 Mk II and f/4 include a 9-bladed curved diaphragm. The diaphragm of the f/2.8 Mk I remains nearly circular from f/2.8 to f/5.6, according to the Canon Press release from 2002. Characteristic of zoom lenses, it exhibits some barrel distortion at its shortest focal length.
An unusual aspect of the f/2.8 Mk I lens (and the 28–70mm f/2.8L) is that its barrel extends as it zooms toward its shortest focal length. When used with the supplied lens hood, which attaches to a non-moving part of the lens, this extension results in a properly matched shade at every angle of view. Most zoom lens hoods are designed for only the widest angle of view, offering progressively inadequate shade at longer focal lengths.
This reverse zoom is illustrated with the following two pictures:
At the telephoto end (70 mm), the end of the lens is the most retracted providing the most shade protection from the lens hood. At the widest end (24 mm), the end of the lens is the most protruding providing the least shade protection from the lens hood.
The f/2.8 Mk II lens, however, has a more conventional design, with the barrel extending as the focal length increases. The lens hood is much shorter than that of the Mk I version. The f/4 lens shares the same conventional design.
The f/2.8 versions are not true 1x macro lenses (1:1); the f/2.8 Mk I has a maximum magnification of only 0.29x and the f/2.8 Mk II has 0.21x. The f/4 lens, while also not a true macro lens, is considerably closer to being in that category. The f/4 has a switch-activated "macro" mode; at 70 mm, the maximum magnification is approximately 0.7x and the closest focusing distance is 20 cm.
|Attribute||f/2.8L USM||f/2.8L II USM||f/4L IS USM|
|Image stabilizer||No||Yes, Hybrid system (4 stops)|
|Zoom Lock Lever||No||Yes|
|Construction||13 groups / 16 elements||13 groups / 18 elements||12 groups / 15 elements|
|# of diaphragm blades||8||9|
|Closest focusing distance||0.38 m / 1.25 ft||0.38 m / 1.25 ft (normal mode)|
0.20 m / 0.66 ft (macro mode)
|Horizontal viewing angle||74°–29°|
|Diagonal viewing angle||84°–34°|
|Vertical viewing angle||53°–19°30'|
|Weight||950 g (34 oz)||805 g (28.4 oz)||600 g (21 oz)|
|Maximum diameter||83.2 mm (3.3")||88.5 mm (3.5")||83.4 mm (3.3")|
|Length||123.5 mm (4.9")||113 mm (4.4")||93 mm (3.7")|
|Release date||November 2002||September 2012||December 2012|
|Currently in production?||No||Yes||No|
- Canon EF 24–105mm lens, available in two versions—one an L class lens, and the other a consumer-level lens—with a similar but slightly longer focal length. The L version has a maximum aperture of f/4 and image stabilization, similar to the 24-70mm f/4L IS USM, but lacks that lens' macro feature. The consumer version is also image-stabilized, but replaces the USM motor drive with STM, has a variable maximum aperture, and also lacks a macro feature.
- Canon EF 70–200mm lens has both image-stabilized and non-IS versions with maximum apertures of f/2.8 and f/4.0, and continues the 24–70mm's maximum focal length through 200mm
- Canon EF 24-70 f/2.8L II Announced « Canon Rumors. Canonrumors.com (7 February 2012). Retrieved 2013-10-04.
- Canon EF 24-70 f/2.8L Version 1 vs Version 2 « Canon Rumors. Canonrumors.com (7 February 2012). Retrieved 2013-10-04.
- Canon EF 24-70 f/2.8L II « Canon Rumors. Canonrumors.com (6 February 2012). Retrieved 2013-10-04.
- "Canon EF 24–70 f/2.8L II Has Made its Way To Retailers". CanonRumors.com. 2 September 2012. Retrieved 12 September 2012.
- "Canon 24-70mm f/4 L IS, Full-Frame EF USM (2012-today)". November 2012. Retrieved 3 October 2013.
- Westlake, Andy (6 November 2012). "Canon EF 24–70 mm f/4 L IS USM Preview". Digital Photography Review. Retrieved 6 November 2012.
- Sample crops
- FM reviews – Canon EF 24–70 mm f/2.8L USM
- Cicala, Roger (11 September 2012). "Canon 24–70 f/2.8 II Resolution Tests". LensRentals.com. Retrieved 12 September 2012.
- Rumors, Canon (8 April 2021). "Canon officially discontinues a lot more EF lenses". Canon Rumors - Your best source for Canon rumors, leaks and gossip. Retrieved 9 April 2021.


text:
Light is good.Reasoning is good, but better to receive what is known by God, than to debate what men only speculate about. We were not left alone. Opinions and and judgments were never to take the place of God. They only light the path for those who walk according to His will. There are also many paths leading to destruction.
I don't believe the bible says that God gives every man the same amount of measure of understanding, or faith, or reasoning.
These are things we need to work out between the individual and the Spirit or amongst a group of likeminded spirit led members.
There is none perfect, no not one..
I believe from time to time God reveals himself in different ways to different people. So everyone gets a piece of the pie and no one person becomes glutoneous.
We know the basics. We have been given the foundation.
But each individual is required to build upon this. We are instructed to be careful how we build upon it though.
Rom 12:3 For I say, through the grace given unto me, to every man that is among you, not to think of himself more highly than he ought to think; but to think soberly, according as God hath dealt to every man the measure of faith.
Rom 12:6 Having then gifts differing according to the grace that is given to us, whether prophecy, let us prophesy according to the proportion of faith;
1Co 3:8 Now he that planteth and he that watereth are one: and every man shall receive his own reward according to his own labour.
Isa 55:8 For my thoughts are not your thoughts, neither are your ways my ways, saith the LORD.
Isa 55:9 For as the heavens are higher than the earth, so are my ways higher than your ways, and my thoughts than your thoughts.
1Co 3:10 According to the grace of God which is given unto me, as a wise masterbuilder, I have laid the foundation, and another buildeth thereon. But let every man take heed how he buildeth thereupon.
Not everyone hears the same way:
Mar 4:24 And he said unto them, Take heed what ye hear: with what measure ye mete, it shall be measured to you: and unto you that hear shall more be given.
If we had it all, then there would be no need to add to the measure. But like a door, I believe God opens our understanding as we are able to receive it.
Eph 4:16 From whom the whole body fitly joined together and compacted by that which every joint supplieth, according to the effectual working in the measure of every part, maketh increase of the body unto the edifying of itself in love.
I like this version NLT:
He makes the whole body fit together perfectly. As each part does its own special work, it helps the other parts grow, so that the whole body is healthy and growing and full of love.
I guess my conclusion would be:
" but better to receive what is known by God, than to debate what men only speculate about. "
No one knows what God has given each individual to know concerning himself.
And what God may be revealing to one person, may appear as speculation on the part of another.
We should agree though, that whatever we add stays within the framework of the original foundation.
And what is that framework?
There is only one God. And we are here to serve Him.
Mic 6:8 He hath shewed thee, O man, what is good; and what doth the LORD require of thee, but to do justly, and to love mercy, and to walk humbly with thy God?

domain: philosophy


In a push for cleaner fuel, India’s petroleum regulator has authorised 33,764 km of natural gas pipeline network for the country’s gas grid.
According to a petroleum and natural gas ministry’s statement, minister of state Rameswar Teli in a written reply in the Lok Sabha said, “As on 31.03.2021, Petroleum and Natural Gas Regulatory Board (PNGRB) has authorized approximately 33,764 Km Natural Gas Pipeline Network across the country with the aim to create a national gas grid and increase the availability of natural gas across the country.”
This comes in the backdrop of India’s push for a gas-based economy. The country consumes around 145 million standard cubic meters a day (mmscmd) of gas.
“The authorised Natural Gas pipeline entity is allowed to lay spurlines as per the provision of the regulations. Accordingly, 19,998 km of Natural Gas pipeline (including sub-transmission pipeline & tie in connectivity pipeline) are operational and 15,369 km are under various stages of construction,” as per the statement.
Gas comprises about 6.2% of India’s primary energy mix, far behind the global average of 24%. The government plans to increase this share to 15% by 2030. India’s gas demand is expected to be driven by the fertilizer, power, city gas distribution, and steel sectors. India’s energy demand is expected to grow at 4.2% per year over the next 25 years.


Invisibl Skratch Piklz - The Shiggar Fraggar Show! Vol. 1 album flac
Title: The Shiggar Fraggar Show! Vol. 1
MP3 album: 1376 mb
FLAC album: 1800 mb
Other formats: VQF FLAC XM ASF VOX WAV DMF
Posted on September 10, 2014December 24, 2018 by DJ Namaximum. Year: 1999 Audio Codec: FLAC . lac) Rip: tracks+. cue Length: 00:55:59. Invisibl Skratch Piklz – 2016 – The 13th Floor. Brian McKnight – 2011 – Just Me. White Mic & Deuce Eclipse – 2016 – The Vegetable & The Bandidos. Papoose – 2015 – You Can’t Stop Destiny. K-Delight – 2003 – Headfucking Breaks – The Wonder Years. OST – 1998 – Def Jam’s Rush Hour Soundtrack. DJ Q-Bert – 1998 – Wave Twisters, Episode 7 – Million Sonic Wars Within the Protons. 1994 – Ready To Die (2006-Remaster). DJ Screw – 1995 – 3 ‘N The Mornin’ (Part One).
Not exactly the Piklz' proper long-form debut, The Shiggar Fraggar Show, Vol. 1 is a live album including segments from a Bay Area pirate-radio show done with ISP-members Mixmaster Mike, Q-Bert, Shortkut, Apollo and DJ Disk. It's just a bit slow out of the gate and doesn't move with the same grace as other fine turntablist moments (like Cut Chemist and Shortkut's Live at the Future Primitive Sound Session), but the volatile scratchwork and entertaining samples keep things interesting. Once the tempo picks up, the album steams ahead with bucketfuls of old-school flavor.
It featured Invisibl Skratch Piklz members Dj Q-bert, Dj Disk and Dj Shortkut, when the Piklz were still widely known as the "West Coast Rock Steady Crew DJs at that time. There is only one criticism to make for this album and that the amount of talking and clowning around on this release, it fun the first time you hear it but by the nine or ten play it get a bit tedious.
Free download and listen THE Shiggar Fraggar Show Vol. . Invisibl Skratch Piklz. Style: rap. Album year: 2005. Tracks count: 23. Views: 1. Invisibl Skratch Piklz - DJ Polar Bear Vs. Shiggar Fraggar 02:17. Invisibl Skratch Piklz - DJ Sly T 01:47. Invisibl Skratch Piklz - Hello Caller 01:31. Invisibl Skratch Piklz - Boxer Shorts Signed By The Village People 00:49. Invisibl Skratch Piklz - Whoever You Are 02:44. Invisibl Skratch Piklz - Raising Snails For A Living 01:23. Invisibl Skratch Piklz - Rock Steady Graffiti Mini-Skirt 03:45. Invisibl Skratch Piklz - Computer Pickles On KKUT 02:20. Invisibl Skratch Piklz - DJ Change Money 03:08.
1 (The Tempers album). Breed 77. Individually and as a group, the Piklz's reputation in the hip-hop underground is undisputed, with journalists regularly describing how they were asked by the Disco Mix Club (DMC), an international DJ association, to stop competing since they were discouraging other DJs from even bothering to enter
|2||Whoever You Are||2:49|
|3||Beware Of The Frozen Toad||2:39|
|4||DJ Polar Bear Vs. Shiggar Fraggar||2:17|
|5||Planet Cow & The Soulsonic Rodeo||2:54|
|6||Six Shots: That Means The Gun's Empty (K-Trash Mix)||3:06|
|7||DJ Change Money||3:06|
|8||Don't Mean To Boast (Ol' Skool Skratch)||3:30|
|9||Raising Snails For A Living||1:23|
|10||Kut Up Musik Best (Doin' The Michael Jackson)||5:44|
|11||Toad Man Vs. Corn Man In "Count Your Vegetables"||1:20|
|12||Invasion Of The Octopus People (Drunken Puss Mix)||4:14|
|13||Rock Steady Graffiti Mini-Skit||3:44|
|14||Computer Pickles On KKUT||2:20|
|15||DJ Sly T||1:47|
|16||Boxer Shorts Signed By The Village People||0:49|
|18||Amadi's Medicore Freestyle ("I Might Sound Shitty But They Are The Best")||0:51|
|19||Shortkut To Disk||3:45|
|20||KKUT 93.7FM, 104.1FM||2:51|
|22||Break It Down Like Dis||1:51|
|23||A Book Of Acid... F-f-f-fres-s-sh||2:09|
- Phonographic Copyright (p) – Hip Hop Slam
- Copyright (c) – Hip Hop Slam
- Design [Graphics] – Dawg Eat Dawg Designz
- DJ Mix – Disk*, Q-Bert*, Shiggar Fraggar, Shortkut
- Mastered By – Stevie K*
- Photography By – Timi D*
- Producer – Billy Jam
NotesMastered at Ice Chamber.
©℗ 2000 Hip Hop Slam Records
On front insert:
"Orchestrated DJ Turntablist Session Strictly for Hardcore DJ Fans"
On trayard insert:
"The Shiggar Fraggar Show! Vol. 1 is a remastered, limited edition CD release from an orchestrated turntablist session featuring DJs Disk, Q-Bert, Shortkut and Shiggar Fraggar, recorded live in March 1995, at Hip Hop Slam, Oakland, CA (renamed "KKUT" for this session) as part of the 100 Pirate Fuckin' Radio series between 1994 and 1999."
"This album is dedicated to the memory of Bay Area Graffiti artist, Mike "DREAM" Francisco (1969 - 2000)"
Barcode and Other Identifiers
- Barcode: 692548801524


INVITATION TO BID
The Taylor County Commissioners’ Court will be receiving sealed bids until 2:00 p.m. on Thursday June 8, 2023 for I. T. B. 2023 -102 Jail Pharmaceuticals. Sealed bids will be received at the Taylor County Purchasing Office, 600 Pecan Street, Abilene, Texas, 79602.
Award will be made on a lump sum basis. Purchase will be on a cash basis.
Exact specifications may be obtained by contacting Scott Henderson, Purchasing Agent, Taylor County Courthouse, 600 Pecan Street, Abilene, Texas 79602, or by calling (325) 738-8602. Bid specifications can also be found on Taylor County’s web page at www.taylorcountytexas.org.
Taylor County reserves the right to reject any or all bids.
EEO - MFHV
Taylor County, Texas


text:
The Impact of Workplace Policies and Other Social Factors on Self-Reported Influenza-Like Illness Incidence During the 2009 H1N1 Pandemic.
Kumar, Supriya PhD, MPH; Quinn, Sandra Crouse PhD; Kim, Kevin H PhD; Daniel, Laura H PhD; Freimuth, Vicki S PhD
American Journal of Public Health.
102(1):134-140, January 2012.
(Format: HTML, PDF)

domain: library


Welcome to visit email@example.com
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text:
According to research, taking a holiday in a sunny climate can seriously damage your intelligence.
Professor Siegfried Lehrl of the University of Erlangen in Germany, a specialist in mental performance, believes that sunbathing and relaxation can cause the frontal lobe of your brain to shrivel.
Infact, indications show that the diminishing of your IQ level could actually begin as soon as you book your holiday. A study by psychologist Glenn Wilson in 2005 revealed that being bombarded by emails can reduce your IQ level by 10 points, so if you book your holiday online and exchange numerous emails with your travel company, your intelligence may begin to wane before you even set off.
Professor Wilson refers to this situation as ‘infomania’ when someone is distracted by the expectance of receiving emails, predicting what they may say and working out how they are going to act on them, all of which distract from whatever this person is actually meant to be concentrating on.
Worrying in the run up to your departure doesn’t help either, the stress of finding passports, not losing tickets, packing, ash clouds and air strikes can cause increased levels of the stress hormone cortisol, which can cause short term memory loss and a lack of concentration.
So we finally arrive on our relaxing holiday, all be it a few brain cells down, and head to the nearest bar, but be careful, that’s another 10 to 20 points off your IQ depending on how much alcohol you drink. Even just an ice cold glass of water could affect your intelligence as researchers at Bristol University discovered that energy and blood are diverted from the brain to the stomach in a bid to counteract the sudden cold temperature.
Inactivity also reduces oxygen levels to the brain and add to this the dehydration from the heat or excess alcohol and your brain cell volume could decrease by up to 15%. A two week holiday in the sun could see your IQ drop enough to turn you from a normally intelligent person to one of just average intelligence according to Professor Wilson, and it can even cause changes in personality.
However, all is not lost and the damage of a holiday in the sun is not irreversible. Further research indicates that it only takes around four days of being back home again for our brain to revert to normal, but perhaps this clears up the mystery as to why we always feel that we are still in ‘holiday mode’ for our first few days back at work.

domain: psychology


Purchase Wholesale Tazers in Mt Vernon NY
A tazer weapon is essentially a portable electroshock gun that was created by a business called tazer global. Most individuals are shocked to find out that the name tazer is actually a phrase that means “Thomas A. Swift’s Electric Rifle.” The tazer weapon was originally developed in 1969 by a developer named Jack Cover that called the electrical weapon after the experience personality Tom Swift.
Just how do Stun Guns Work?
Tazer weapons function by shooting small dart-like electrodes that are driven by small gas charges (such as pressed nitrogen). The electrodes have steel cords that connect back to the device. When the electrodes make contact with the target, the digital gadget transfers powerful electric pulses along the cables as well as right into the body of the target, causing substantial pain. The maximum variety of the common weapon is about 30 feet. The weapons utilize a shaped pulse arc of power that interferes with an individual’s nerve as well as muscle mass function without requiring steel prongs on the projectile to penetrate the skin.
The pulse versions are created to bring down a topic even if they are wearing body armor.
Who Uses the Self Defense Tools in New York?
Tazer weapons are utilized mostly by police divisions as well as law enforcement officers in an initiative to reduce the number of deadly firearm-related fatalities. Lots of police departments report a decline in the number of authorities officer-involved capturings as an outcome of utilizing the authorities tazer guns.
Controversy over Security
There is growing conflict concerning the usage, or abuse of the digital guns, particularly worrying using authorities tazers. While they are not practically thought about dangerous tools, many question the safety as well as humaneness of the devices. Many individuals feel making use of police tazer weapons is abused. A number of civil liberty teams would like to see them outlawed totally. In fact, Amnesty International has recorded over 150 deaths related to the use of electrical weapons.
Stun Gun Accessories 10552
Numerous spy equipment stores also sell tazer holsters,, stun batons, and belts. Holsters are typically unnecessary for the average individual, as the majority of private citizens wish to hide their tool. Nonetheless, authorities as well as safety professionals could put on a holster as a part of their standard attire.
Laws as well as Restrictions in New York
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The electronic tools are limited in the list below countries: Australia, Belgium, Canada, Denmark, Hong Kong, India (permitted for police utilize only), Italy, Japan, New Zealand, Norway, Sweden, Switzerland, UK.


text:
What is Percocet?
Percocet is a strong prescription pain medicine that contains an opioid (narcotic) that is used to manage pain, severe enough to require an opioid analgesic and for which alternative treatments are inadequate and when other pain treatments such as non-opioid pain medicines do not treat your pain well enough or you cannot tolerate them. it is an opioid pain medicine that can put you at risk for overdose and death. Even if you take your dose correctly as prescribed you are at risk for opioid addiction, abuse, and misuse that can lead to death.
Oxycodone Hydrochloride and Acetaminophen is available in tablets for oral administration.
Each tablet for oral administration, contains oxycodone hydrochloride and acetaminophen in the following strengths:
Oxycodone Hydrochloride, USP 2.5 mg*
(*2.5 mg oxycodone hydrochloride is equivalent to 2.2409 mg of oxycodone.)
Acetaminophen, USP 325 mg
Oxycodone Hydrochloride, USP 5 mg*
(*5 mg oxycodone hydrochloride is equivalent to 4.4815 mg of oxycodone.)
Acetaminophen, USP 325 mg
Oxycodone Hydrochloride, USP 7.5 mg*
(*7.5 mg oxycodone hydrochloride is equivalent to 6.7228 mg of oxycodone.)
Acetaminophen, USP 325 mg
Oxycodone Hydrochloride, USP 10 mg*
(*10 mg oxycodone hydrochloride is equivalent to 8.9637 mg of oxycodone.)
Acetaminophen, USP 325 mg
All strengths of PERCOCET also contain the following inactive ingredients: Colloidal silicon dioxide, croscarmellose sodium, microcrystalline cellulose, povidone, pregelatinized cornstarch, and stearic acid. May also contain crospovidone. In addition, the 2.5 mg/325 mg strength contains FD&C Red No. 40 Aluminum Lake and the 5 mg/325 mg strength contains FD&C Blue No. 1 Aluminum Lake. The 7.5 mg/325 mg strength contains FD&C Yellow No. 6 Aluminum Lake. The 10 mg/325 mg strength contains D&C Yellow No. 10 Aluminum Lake. The 7.5 mg/325 mg strength and the 10 mg/325 mg strength may also contain corn starch.
Oxycodone Hydrochloride and Acetaminophen Tablets contain oxycodone, 14- hydroxydihydrocodeinone, a semisynthetic opioid analgesic which occurs as a white to off-white fine crystalline powder. The molecular formula for oxycodone hydrochloride is C18H21NO4 ∙ HCl and the molecular weight is 351.82. It is derived from the opium alkaloid, thebaine, and may be represented by the following structural formula:
Oxycodone Hydrochloride and Acetaminophen Tablets contain acetaminophen, 4′-hydroxyacetanilide, is a non-opiate, non-salicylate analgesic and antipyretic which occurs as a white, odorless, crystalline powder. The molecular formula for acetaminophen is C8H9NO2 and the molecular weight is 151.16. It may be represented by the following structural formula:
Acetaminophen Structural Formula
Mechanism of Action
Oxycodone is a full opioid agonist with relative selectivity for the mu-opioid receptor, although it can interact with other opioid receptors at higher doses. The principal therapeutic action of oxycodone is analgesia. Like all full opioid agonists, there is no ceiling effect for analgesia with oxycodone.
Clinically, dosage is titrated to provide adequate analgesia and may be limited by adverse reactions, including respiratory and CNS depression.
The precise mechanism of the analgesic action is unknown. However, specific CNS opioid receptors for endogenous compounds with opioid-like activity have been identified throughout the brain and spinal cord and are thought to play a role in the analgesic effects of this drug.
The precise mechanism of the analgesic properties of acetaminophen is not established but is thought to involve central actions.
What is the most important information I should know about Percocet?
- Get emergency help right away if you take too much Percocet (overdose). When you first start taking Percocet, when your dose is changed, or if you take too much (overdose), serious or life-threatening breathing problems that can lead to death may occur. Taking Percocet with other opioid medicines, benzodiazepines, alcohol, or other central nervous system depressants (including street drugs) can cause severe drowsiness, decreased awareness, breathing problems, coma, and death.
- Never give anyone else your Percocet. They could die from taking it. Store Percocet away from children and in a safe place to prevent stealing or abuse. Selling or giving away Percocet is against the law.
Who should not take Percocet?
Do not take Percocet if you have:
- Severe asthma, trouble breathing, or other lung problems.
- A bowel blockage or have narrowing of the stomach or intestines.
- Known hypersensitivity to oxycodone, acetaminophen, or any ingredient in Percocet.
What should I tell my healthcare provider before taking Percocet?
Before taking Percocet, tell your healthcare provider if you have a history of:
- Head injury, seizures
- Liver, kidney, thyroid problems
- Problems urinating
- Pancreas or gallbladder problems
- Abuse of street or prescription drugs, alcohol addiction, or mental health problems
Tell your healthcare provider if you are:
- Pregnant or planning to become pregnant. Prolonged use of Percocet during pregnancy can cause withdrawal symptoms in your newborn baby that could be life-threatening if not recognized and treated.
- Breastfeeding. Percocet passes into breast milk and may harm your baby.
- Taking prescription or over-the-counter medicines, vitamins, or herbal supplements. Taking Percocet with certain other medicines can cause serious side effects that could lead to death.
How should I take Percocet?
When taking Percocet:
- Do not change your dose. Take Percocet exactly as prescribed by your healthcare provider. Use the lowest dose possible for the shortest time needed.
- Take your prescribed dose every 6 hours as needed for pain. Do not take more than your prescribed dose. If you miss a dose, take your next dose at your usual time.
- Call your healthcare provider if the dose you are taking does not control your pain.
- If you have been taking Percocet regularly, do not stop taking Percocet without talking to your healthcare provider.
- After you stop taking Percocet, dispose of unused tablets by flushing them down the toilet.
What should I avoid while taking Percocet?
While taking Percocet do not:
- Drive or operate heavy machinery, until you know how Percocet affects you. Percocet can make you sleepy, dizzy, or lightheaded.
- Drink alcohol or use prescription or over-the-counter medicines that contain alcohol. Using products containing alcohol during treatment with Percocet may cause you to overdose and die.
What are the possible side effects of Percocet?
The possible side effects of Percocet include:
Call your healthcare provider if you have any of these symptoms and they are severe.
Get emergency medical help if you have:
- trouble breathing
- shortness of breath
- fast heartbeat
- chest pain
- swelling of your face, tongue, or throat
- extreme drowsiness
- light-headedness when changing positions
- feeling faint
- high body temperature
- trouble walking
- stiff muscles
- mental changes such as confusion
These are not all the possible side effects of Percocet. Call your doctor for medical advice about side effects.
You may report side effects to FDA at 1-800-FDA-1088. For more information go to dailymed.nlm.nih.gov.
General information about the safe and effective use of Percocet
Medicines are sometimes prescribed for purposes other than those listed in a Medication Guide. Do not use Percocet for a condition for which it was not prescribed. Do not give Percocet to other people, even if they have the same symptoms you have. It may harm them. You can ask your healthcare provider or pharmacist for information about Percocet that was written for healthcare professionals.
How should I store Percocet?
- Store at 20º to 25ºC (68º to 77ºF)
- Protect from moisture
- Store in a tight, light-resistant container
- Keep out of reach of children
What are the ingredients in Percocet?
Active ingredients: oxycodone hydrochloride, acetaminophen
5mg tablets: silicon dioxide, croscarmellose sodium, crospovidone, cellulose microcrystalline, povidone, starch corn, stearic acid, FD&C blue No.1
7.5mg tablets: silicon dioxide, croscarmellose sodium, crospovidone, cellulose microcrystalline, povidone, starch corn, stearic acid, FD&C yellow No.6
10mg tablets: silicon dioxide, croscarmellose sodium, crospovidone, cellulose microcrystalline, povidone, starch corn, stearic acid, D&C yellow No. 10
PACKAGE LABEL – PRINCIPAL DISPLAY PANEL – 5 MG/325 MG 100 COUNT BOTTLE
SRC: NLM .

domain: medical


text:
Grey Wall Paint. Bathroom design by jillian lare. Solid grey wall colour moving towards slightly darker greys, this solid grey wall paint works well in a bedroom space where it creates a beckoning atmosphere when paired with a classic white and black combination.
Living room paint color ideas with shades of lilac. If you dont have the space or the energy for a bevy of house plants green walls will be the splash of life your living room needs. Sherwin williams agreeable gray sw7029.
Paint Brands Featured Below Include :
It’s a deep, sumptuous shade of gray that wraps you like a velvety fog. The reason blue gray paint colors are great is because when they are paired with brighter colors they will act as neutrals. It's popular in many modern situations and when used on walls, trim or floor can make them look like new.
Grey Paint Is Seen As A Cool, Neutral Colour And The Intermediate Colour Between Black And White.
Solid grey wall colour moving towards slightly darker greys, this solid grey wall paint works well in a bedroom space where it creates a beckoning atmosphere when paired with a classic white and black combination. Grey provides a wonderful base for your home to create a stylish and elegant environment. Please visit our paint color family pages.
On Your Walls, A Light Gray Paint Can Present As A Subtle Backdrop And A Dark Gray Paint Acts As A Dramatic Focal Point.
In general, gray paint comes in warm or cool hues, ranges from very light to very dark, and can take. Sherwin williams agreeable gray sw7029. It’s a warm stony color right in between true gray and beige.
It Has The Ability To Be The Supporter Of A Bolder Colour Or The Stand Alone Colour That Transfaroms And Modernises Your Home.
16 lilac wall paint and purple corner sofa. Paint walls in olive sprig to encourage focus in an office, incorporate soothing color into a bathroom, or promote restfulness in a bedroom. A great finish always starts with preparation so make sure your paint job begins with a good.
The Elegant Looking Dining Area Is Subtly Complemented By A Bit Of Warm Grey Wall Paint On One Of The Walls.
Bathroom design by jillian lare. Some of these livid colors are more blue than gray and others are more gray than blue. Used as the color for your walls, gentle gray can work for any room.

domain: painting