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Saturdays, September 6, 13, 20 at 3:00pm:
Perfect Little Planet
Imagine the ultimate space vacation! Discover our solar system through a new set of eyes – a family from another star system seeking the perfect vacation spot. Fly over the surface of Pluto, our best known Dwarf Planet. Dive over the ice cliffs of Miranda. Sail through the rings of Saturn. Feel the lightning storms of Jupiter. And walk on the surface of Mars. Which destination would you choose? A solar system journey for space travelers of all ages.
Watch a preview
Saturdays, September 6, 13, 20 at 7:00pm:
Back to the Moon for Good
(Grades 3 to adult and families)
Immerse yourself in a race to the Moon 40 years after the historic Apollo landings. Learn about the history of lunar exploration, and the Moon’s resources. Discover what humanity’s future on the Moon might hold. See how a competition among privately funded international teams is ushering in a new era of lunar exploration. Narrated by Tim Allen, BACK TO THE MOON FOR GOOD presents the Google Lunar XPRIZE, and the personal stories of competition and collaboration it inspires.
Watch the trailer
Thursdays, September 4, 11, 18, 25 at 7:00pm:
(Grade 5 to adult and families)
From the edge of space and time, through vast fields of wondrous galaxies, to the majesty of our solar system, this show will take audiences on a grand tour of the universe and let them experience its most provocative secrets.
Ultimate Universe is a three dimensional journey from the edge of the universe through space and time to reach our home planet, witnessing the major components of the cosmos along the way. First, tour the night sky as seen from the Earth. Then, peer deep into space through the eyes of the orbiting Hubble Space Telescope and travel back billions of years in time to witness the birth of the universe. On this breathtaking excursion, you’ll witness the formation of galaxies and explore some of the most wondrous nebulae and astronomical structures yet discovered. As your travels continue, you’ll fly deep into our own Milky Way galaxy and return home to Earth on a spectacular tour through the solar system.
Produced by Evans & Sutherland Digital Theater Productions & Clark Planetarium Productions.
Watch the trailer
Halloween Special Shows
October 17 and 24 at 7:00 pm
A classic legend of the night sky involving a Princess, a Hero,
Monsters, and more. Also included are fun short segments from
Animalopolis, Enchanted Reef, and Scary Cave.
October 18 and 25 at 7:00pm
Incredible full-dome graphics and the classic rock soundtrack by Pink Floyd.

domain: astronomy


text:
You are here
Opera and lyrical music
Sheet music for Leo Delibes
String Quartet, String Orchestra - Advanced Intermediate - Digital Download
Composed by Leo Delibes (1836-1891). Arranged by Stephen M. Taylor. Opera, Repertoire, Technique Training, Wedding, Recital. Score, Set of Parts. 24 pages. Published by SMT Music (S0.185831).
Woodwinds Flute 1, Flute 2 (soprano)
From Lakme. Composed by Leo Delibes (1836-1891). Arranged by Margaret Baxtresser (piano reduction) Jeanne Baxtresser. Sws. Performance Notes by Jeanne Baxtresser The duet Viens, Mallika from Leo Delibes opera Lakme is one of the most enchanting pieces of music I have ever heard. Realizing it would make an equally beautiful duet for two flutes, I made this transcription to hav. Classical. Set of parts. With Standard notation. Composed 1998. 8 pages. Duration 3 minutes, 45 seconds. Theodore Presser Company #114-41019. Published by Theodore Presser Company (PR.114410190).
Orchestra Piano (Orchestra) - Digital Download
Classical. Individual instrument part. 4 pages. Published by Hal Leonard - Digital Sheet Music (HX.250414).
Flute, Violin, Harp, 2 Flutes and Harp or Violin(s) and Harp - Early Intermediate - Digital Download
Composed by Leo Delibes (1836-1891). Arranged by Monty and Phyllis Adams. Romantic Period, Opera, Wedding, Funeral, Recital. Score, Set of Parts. 7 pages. Published by Adams Magnificent Music (S0.18411).
Piano/Vocal/Guitar (chords only) - Digital Download
Classical. Piano/Vocal/Guitar (chords only). 6 pages. Published by Hal Leonard - Digital Sheet Music (HX.365855).
Flute, Oboe, Clarinet, Bassoon, Woodwind Quintet, Horn in F - Intermediate - Digital Download
Composed by Leo Delibes (1836-1891). Arranged by Mike Nelson. Romantic Period, Opera, Impressionistic, Wedding, Recital. Score, Set of Parts. 20 pages. Published by Mike Nelson (S0.174333).
The composer was born Clément Philibert Léo Delibes in Saint-Germain du val, February 21, 1836. He began his musical studies in 1847 at the Paris Conservatoire, and began voice training in 1848. He studied composition under Adolphe Adam (Giselle) and became accompanist and chorus master for the Théâtre-Lyrique, and then, in 1864, became second chorus master at the Paris Opéra. He was also organist at Pierre de Chaillot from 1865 to 1871. In 1871 he married Léontine Estelle Denain.
His first stage work was the light opera Deux sous de charbon (Two Pennies Worth of Coal), which was performed at the Follies Nouvelles in 1856. He continued to compose light operas and vaudevilles at the rate of about one per year for the next fifteen years. The success of a ceremonial cantata, Alger, for Napoleon III led to his collaboration with Ludwig (Léon) Minkus on Delibes’ first ballet composition, which was to become La Source, in 1866. It was a ballet with an Oriental theme, which was very fashionable at the time. Delibes’ contributions of Act 2 and the first scene of Act 3 were considered the superior compositions of the work.
Delibes was asked to compose a waltz, Valse, ou pas des fleurs, that was added into the 1867 revival of Adam’s Le Corsaire. This music was combined with his music for La Source to accompany Soir de Fête (Gala Night) for the Paris Opéra Ballet. Choreographed by Léo Stats, the work was seen more than 250 times. The thirty-year-old Delibes was assigned to collaborate on Coppélia with the more-senior Nuitter and Saint-Léon, with whom he had already worked creating La Source. Little is known about the way in which they worked together, but it’s believed that the whole grew out of the Nuitter’s scenario.
Not only is Coppélia notable as Delibes’ first complete ballet score written solo, but it’s also noted as a work that moved ballet music forward in a major step. Delibes provided a generous amount of expressive character in his music — a new idea at the time — to produce music that is a descriptive tone poem. Many find that his music contains early impressionist elements, as well as the new, more sophisticated use of the leitmotif. Sylvia (1876) is considered Delibes’ best ballet — and in fact, the best ballet music before Tchaikovsky, who judged it to be better than his own Swan Lake.
Neither Coppélia nor Sylvia was published in other than piano version at the time of their composition, and that has given rise to a number of different arrangements of the music. Most current productions, however, refer to Delibes’ original version. Indeed, the longevity of the ballet is more often attributed to the strength of the music than to the choreography.
In 1881 Delibes became professor of composition at the Paris Conservatoire. His only other balletic composition was a suite of six dances for a Comédie-Francaise production of Le Roi s’Amuse in 1882. He wrote three operas, including, in 1883, Lakmé, which quickly gained in popularity on both sides of the Atlantic, becoming almost as famous as Bizet’s Carmen.
Delibes died in Paris, January 16, 1891.
(Contribution by <email@example.com>.)

domain: library


KEMPEROL® AC Speed FR is a quick-curing, cold liquid-applied Polymethyl Methacrylate (PMMA) waterproofing resin that can allow same-day application. The membrane is fully reinforced with a needle-punched polyester fleece that provides exceptional durability for exposed or buried roofing & waterproofing applications. The resin is available in two standard colors, gray or a highly reflective white with an SRI of 108.
For roofing applications, KEMPEROL® AC Speed FR system can be applied exposed, direct-to-deck, over an insulated assembly, or recover an existing membrane. The self-flashing membrane is ideal for inverted roof applications as there are no seams or termination bars used.
When used for waterproofing, the membrane can be covered with tile, paver, pavement, growing media and other types of approved overburden. Optional KEMPERDUR® AC Traffic Coating allows for additional surfacing of balconies, terraces, walkways and parking decks with the quick-cure performance.
|traffic gray or white
|cures to solid
|Thickness (120 fleece)
|Water Vapor Transmission
|Solid to walk on after*
|Apply surfacing/Coating after*
|Apply overburden after*
|Completely hardened after*
|2 mm/0.08 inch
Short-term temperature resistance
|250 °C/ 482 °F
* all times are approximate and depend upon air flow, humidity and temperature


Weather delays utility project on Vail’s frontage road
Vail, CO Colorado
VAIL, Colorado – April snows have pushed water utility work in Vail into the week of April 25.
Late season snowstorms have caused daily delays to the work occurring along South Frontage Road in Vail between Ford Park’s eastern parking lot and Vail Valley Drive since the project began on April 18.
A utility box and associated equipment is being installed and connected to the water distribution system as part of Eagle River Water and Sanitation District upgrades to the Vail public water system. The project includes installation of additional piping, remote water quality analyzers, and upgraded equipment at seven sites throughout Vail and is required to comply with the Environmental Protection Agency’s new Ground Water Rule regulations.
Single-lane closures of South Frontage Road for about one-half mile within the project area will continue April 25 for several days until the work is completed. Traffic control measures are in place, with flaggers to direct traffic around the closed lane.
For project construction information, call John Cahill, construction manager, at 970-471-5357 or visit the district’s website at http://www.erwsd.org.


Yeastar Neogate TG100 – 1 Port GSM VoIP Gateway
eastar NeoGate TG100 is a fully featured 1 port VoIP GSM gateway that provides GSM network connectivity for softswitch and IP PBX. It significantly reduces the costs of calls with two-way communication: VoIP to GSM and GSM to VoIP. With friendly GUI, everything can be easily set up.
Yeastar TG100 VoIP GSM Gateway Highlights:
- Calling Type – termination (VoIP to GSM), origination (GSM to VoIP)
- Intelligent call routing
- Callback function
- SMS sending and receiving (Web interface)
- LCR (least cost routing)
- Simple web based configurations
- Cost Savings – Save phone bills tremendously with mobile-to-mobile calls and LCR.
- Back up – Work as a cost-effective backup when the landline network goes down.
- Easy to install – Everything can be easily set up in the Web based management interface.
- Easy to integrate – High compatibility with major IP PBX and softswitch brands (Asterisk, MS Lync, Trixbox, Elastix, FreeSWITCH, 3CX)
Yeastar TG100 Gateway Specs
- Number of GSM channels (Max): 1
- Network type: 850/900/1800/1900MHz
- Protocol: SIP (RFC3261), IAX2
- Transport Protocol: UDP,TCP,TLS,SRTP
- DTMF: RFC2833, SIP INFO, In-band
- Codec: G.711 a/u-law, G.722, G.726, G.729a, GSM, ADPCM, Speex
- Echo Cancellation: ITU-T G.168 LEC
- LAN: 1 (10/100 Mbps)
- Network: Static IP, DHCP Client, Firewall, VLAN, DDNS, QoS, OpenVPN
- NAT Traversal: Static NAT, STUN
- Size: 110x70x24mm
- Power Supply: AC 100~240V/50~60Hz (DC 12V, 1A)


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Devita, High Performance Glycolic Acid Blend, 1.7 oz (50 ml)
Devita, High Performance Glycolic Acid Blend, 1.7 oz (50 ml)
- You earn loyalty credit for every order you place at iHerb!
- Loyalty credit is equal to 5% of the value of your order, excluding shipping charges.
- Your loyalty credit will be applied towards your next order as soon as your current order has shipped.
- Your loyalty credits are valid for up to 60 days from the date of your last purchase.
Expiration Date:Expiration Date
The length of time for the expiration date or "best used before" date depends on the type of product, as well as the brand.
Perishable items (such as flax oils or certain probiotics) generally have shorter expiration dates. Although our warehouse is fully air-conditioned, these more fragile items are put in cold storage (freezer or refrigeration unit) for maximum freshness.
Our receiving department does its best to verify and then enter the correct expiration dates for all incoming products. However, discrepancies do occur from time to time. This being said, the exceptionally high turnover at iHerb ensures that our inventory is among the freshest in the industry.
Shipping Weight:0.59 lbsSwitch to Metric units
- Product Code: DVA-12343
- UPC Code: 682941123439
- Package Quantity: 1.7 oz (50 ml)
1.5 x 1.5 x 6.3 in, 0.35 lbsSwitch to Metric units
|Combo Discount:||- $4.00|
- Devita Natural Skin Care
- Paraben Free
- 100% Vegan
- "Guided by Nature, Driven by Science"
The DeVita Story...
For nearly my entire life my skin had suffered from chronic cystic acne, and then when my 40's hit, so did the ravages of the Arizona sun I had lived in all my adult life. After spending many hundreds of dollars on expensive "name brand" products, which did nothing except break down my bank account and break out my face, I decided to u se my background in chemistry to "deconstruct" those well known high priced potions. I discovered there was nothing in them that would do what they claimed, nor was there any ingredient used that should be so costly. And then, to top it all off, they were all loaded with poisons and preservatives!
After that sad discovery, I knew I had to take matters into my own hands. At that time, I had a small essential oils company, and I began working with organic aloe vera as a dynamic delivery system together with cutting edge advancements in active natural ingredients to create the beginnings of an effective natural products line. So after exhaustive and extensive research and development - DeVita Natural Skin Care was born!
Our basic philosophy?
Make it Clean • Make it Work • Make it Natural • Make it Exceptional... Make it DeVita!
In love and in health,
For over ten years now DeVita has been dedicated to developing beautifully pure age defying skin care products. DeVita now brings that care and expertise to their exclusive line of color cosmetics - [ab.so.lute] minerals
Just great, healthy coverage you can count on absolutely...
Ask for it by name.
From Caterpillar to Butterfly...
Reveal your inner beauty with our natural Glycolic Acid Blend formula. Glycolic acids have long been shown to promote rapid change in rough, listless skin by encouraging the rate of cellular turnover which can bring the appearance of smoother, younger looking skin, reduced pore size, and give a "blushed", healthy glow. Assists in minimizing break-outs, and milia, reduces the appearance of fine lines, deeper wrinkles and darkened areas on the face. Our exclusive formula uses the latest in gel technology featuring our all-natural source of fruit acid blend imported from Italy. So be beautiful, butterfly!
For evening use only
First 7 to 10 evenings: After cleansing and toning-massage a thin amount on face until absorbed.
On evening 8 to 11 and on: Use one to three times weekly as needed for desired results.
Aloe barbadensis* (aloe vera gel), de-ionized water, carthamus tinctorius (safflower) seed oil, camellia sinensis (japanese green tea) leaf extract, lactic acid (natural fruit acid), maleic acid (natural fruit acid), glycolic acid (natural fruit acid), allantoin, chitin (mushroom source), zinc stearate, panthenol (vitamin B5), silybum marianum (st. mary's thistle), hydroxymethylcellulose, squalane (olive), sodium PCA, hydrolyzed wheat protein, lecithin phospholipid.
Ingredient labeling adheres to international INCI standards.
Gluten Sensitive Safe
* Certified Organic
Bought this as a closer-to-nature replacement for Neo Strata Smoothing Cream 10%AHA, which I had been using for some years. Had no irritation whatsoever, except when I tried layering a moisturizer over the DeVita gel, so best used as a stand-alone product. Love it!
Lovely product BUT...
Posted by iHerb Customer on May 28, 2017 | Verified Purchase
I must admit i was very pleased with the texture of the product , which is gel-like . It makes it a great product for the combo or oily skin . But the thing is i was expecting it to be much stronger with AHA content.
Posted by 5457763056959920293 on Oct 28, 2016 | Verified Purchase
My skin looks and feels good after using the product in a week. Now im using it every other day
So far so good
Posted by 5652048142629969857 on Aug 06, 2016 | Verified Purchase
I have only been using this for 2 weeks and already see fine lines disapperaing - I am 49 and l have quite oily skin with larger pores You really only need one pump of the bottle per use "less is more"
Posted by ElinorSimner on Jul 19, 2016 | Verified Purchase
Honestly, it's completely not that I was expecting from this product. It's nice, gentle, but it didn't make any changes to my skin. I have mild acne around my jawline area and my forehead. When I first put it on my face it didn't even tingled like it suppose to. I think that the percentage of Glycolic acid here is very small. Won't purchase again. המוצר בהחלט לא ענה על הציפיות שלי. אני רגילה לתחושת עקצוץ לאחר מריחת חומצה גליקולית. בפעם הראשונה שמרחתי אותו על הפנים שלי, לא הרגשתי שום דבר.אני חושבת שהסיבה לכך היא אחוז מאד נמוך של החומצה. באופן כללי הוא די נחמד,עדין ואולי יתאים לבעלות עור נורמלי ולא בעייתי. אני אישית בעלת עור רגיש ונוטה לפצעונים.,במיוחד בתקופת הווסת.לא חושבת שארצה לרכוש את המוצר הנל שוב.
Lots of botanicals, not much glycolic acid
Posted by 4749231148983821408 on Jun 30, 2016 | Verified Purchase
This is a decent glycolic acid serum for the price, but compared to the previous one i was using, (MUAC Gly-Luronic), it really lacks in strength. Fine for maintaining my relatively clear skin, but if you want something to use on breakouts or to noticeably refresh your skin overnight - it won't have the same results. Also the huge ingredients list of botanicals means it's way more likely to contain something you might have a reaction too. All in all good, but only something I will buy again if I don't have other options. (Packaging is lovely however. The glass bottle is very nice. Also absorbs quickly and doesn't sting or leave a residue.)

domain: beauty


text:
The environmental challenges our planet faces are, to say the least, complicated. Solving them requires cooperation, most powerfully through local civic participation. Environmental Action Civics embodies the belief that participation in […]
Earth Force sends huge congratulations to NOAA and the B-WET program on its 20th anniversary this year. B-WET is an incredible force in environmental literacy – since 2002, the B-WET […]
The value that young people can bring to making our schools and communities more resilient to climate change deserves more than a single sentence in a three-page feature. Young people should be at the center of our resilience efforts. There is no better way to learn math, language, science, and other subjects. Nothing is more important than teaching young people how to employ that knowledge in service to our future.
Earlier this week the IPCC released their latest report on climate change. The report is sobering. The news is that even if we immediately reduce greenhouse gasses as much as […]
When Mary Breslin’s seventh-grade science class at George Washington Middle School in Alexandria, Virginia, met for the first time in fall of 2018, several students noticed something strange: a mushroom […]
Over the last few years, a growing group of environmental education organizations has come to the conclusion that the scale and complexity of the environmental challenges we face require more […]

domain: sociology


text:
Ellen Geer born on 29 August 1941 in New York City to Will Geer and Herta Ware. She is a citizen of United States of America.
She is an Actress, director.
Ellen Geer has had two spouses: Ed Flanders and Peter Alsop. She has two children Ian Geer Flanders and Willow Geer-Alsop.
Ellen Geer has worked as a film director, screenwriter, teacher, stage actor, film actor, television actor and theatre director.
She is also known as Ellen Ware Geer.

domain: entertainment


text:
Archival Resources Details Royal Society of Tasmania
- Collection Title
- Royal Society of Tasmania
- Tasmanian Archive and Heritage Office TAHO, Tasmanian Government
- Date Range
- 1843 -
The Tasmanian Government Archives holds an extensive collection of materials relating to the Royal Society of Tasmania. There are five series in this collection relating to Annual Reports, Minutes, and Burials.
- Finding Aid

domain: history


text:
“Amazing”, “tremendous”, “great” and “fantastic”.
That's how United Autosports drivers Martin Brundle, Mark Blundell, Mark Patterson and Zak Brown described the team's U.S. debut at the Rolex 24 at Daytona ending the race in fourth place – just three seconds behind the overall leader.
The car – in Brundle's hands – entered the final one lap shootout in fourth, and while a “banzai” move from Brundle around the outside of turn one, attempting to take third and/or second, only led to the ex-F1 driver running wide fourth was still a well deserved result at the end of the twice-around the clock race.
The race was far from trouble free for the team. Martin Brundle was one of many, many drivers to bring a car back with flat right-rear tyre – the team's telemetry indicating a slow leak before any damage could be done to the car. The nose of the United Autosport with Michael Shank Racing, Riley chassised car was also replaced – three times – after various incidents, including Brundle running over a large piece of debris on the track.
These delays put the car three laps off the pace, but with strategy the team were able to climb back onto the lead lap, in fifth place in the closing third of the race.
In the remaining stints Blundell and Brundle were part of a three-way battle for the lead with podium finishers Chip Ganassi Racing and Action Express Racing – the three cars following each other round the track often separated by less than a second. Brundle, taking the car to the end of the race would fall to fourth, a recovering no.01 getting past into the chicane on the way to winning the race, but though missing out on a podium the team's performance was still one of the stories of the race.
“We have two retired pros in Mark and I plus two Pro-Am drivers up against car line-ups featuring four current hot shoes,” said Brundle. “I'm kind of disappointed but that is typical me of me – I wanted that podium. My goal was a top-10 on the grid and top-five in the race and we achieved both so I think we covered ourselves with a bit of glory and maybe surprised more than a few people.”
The race also included a few personal milestones and accolades for the drivers. Mark Patterson led a race for the first time in a seven year Daytona Prototype career and Zak Brown – team co-owner with Richard Dean as well as driver – received the Jim Trueman Award for being the top placed Pro-Am driver.
“Fourth place and to be on the lead lap, fighting for a podium with a chance for us winning on our Daytona dÃ©but and our first race in the US, is fantastic,” enthused Brown. “The two Marks and Martin did a great job – I couldn't be happier. Of course it is a little disappointing not to get on the podium when we were a couple of feet away but hey, we'll take a fourth in this field.”
The team has not ruled out further Grand-Am races.

domain: sports


text:
THE KNIX FACTOR!
Reality babe Amelia flashes her pants
SINGING sensation Amelia Lily flashed her KNICKERS as she arrived at The X
Factor end-of-season party. The Middlesbrough babe’s white pants were on display for photographers waiting outside the exclusive DSTRKT club in London.
The beaten finalist is hoping to extend her 15 minutes of fame, and what better way to get some attention than flashing your pants.
Walking along with a huge grin on her face, Amelia appeared to be oblivious as she hooked her beige dress up to reveal her undies.
The singer, who was also dressed in a black leather jacket, had decided to bare her legs despite the wintry weather.
And the 17-year-old may live to regret her choice as she grasped her blue clutch bag, as well as the hem of her skirt.
One onlooker said: “Amelia might not have won the competition but she’s number one for flashing.
“When her knickers made an appearance there was instant round of applause. Hopefully she’ll make a point of doing this at all future public appearances.”

domain: celebrity


Let the games begin-the games of chance.
The launching last week of the Chicago-area`s first floating casino raised the ante in gambling options available locally. It also raised the hopes of two suburbs: Joliet and Aurora.
The two river towns are hoping to hit the jackpot with riverboat gambling. Gaming revenue is seen as a way to revitalize their old, tired downtowns.
Spinoffs from the gambling-driven boom in tourism could benefit new restaurants, shops and hotels, and increase property values.
Already millions of dollars have been committed to the construction of docking facilities and riverside improvements in both cities.
''Nobody is going to solve all urban problems with gaming, but this is a step in the right direction,'' said William Sabo, president of the Empress River Casino Corp.
His firm`s 1,000-passenger Empress is the first of four boats that will offer gambling cruises on rivers in the Chicago area.
Another Joliet boat, this one to be docked downtown, is expected to be launched next spring. And next June two identical gaming craft are scheduled to weigh anchor from downtown Aurora on the Fox River, 40 miles west of the Loop.
Looking as sleek as a yacht from a James Bond movie, the 222-foot-long Empress began a limited cruise schedule last Wednesday after being approved by the Illinois Gaming Board. Full operation will be inaugurated June 28, according to Sabo.
Docked on the Des Plaines River, three miles from downtown Joliet and 43 miles southwest of Chicago`s Loop, the white gambling boat-a rakish, simulated catamaran-recently arrived in Joliet after being built in Florida at a cost of $12.5 million.
The floating casino would satisfy James Bond`s penchant for games of chance, with more than 600 slot machines, 35 blackjack tables, five craps tables, three roulette wheels, mini-baccarat, a wheel of fortune and video poker.
The Empress River Casino Corp. has put some $34 million into its project, which includes a 44,000-square-foot docking pavilion and a 1,500-car parking lot at the 55-acre riverside site off U.S. Highway 6, southwest of downtown but within the city limits.
''Riverboat gambling will start the rebirth of Joliet`s center,''
predicted John Mezera, Joliet`s city manager. ''It will save us five years in time and effort in redeveloping our downtown.''
He said the downtown boat, which is a venture of Harrah`s Casinos, owned by the Promus Cos. of Memphis, represents an immediate investment of $55 million, including the boat, a 40,000-square-foot docking pavilion and a 300- car parking deck.
''We anticipate another $45 million in private investment to occur within two to five years,'' Mezera said. ''A new downtown hotel is high on our wish list. We`ve had two or three serious proposals, and possibly construction could start in 1993 and be completed in 1994.''
Tim Wilmott, general manager of Harrah`s Casino Cruises Joliet, said the operation should gross $30 million to $40 million a year.
Donald Fisher, Joliet`s director of planning, said the city expects to reap as much as $5 million annually from its two gaming operations.
In Aurora, Mayor David Pierce predicts an annual return of $2 million to the city, based on a conservative estimate of 500,000 visitors a year to the two boats to be operated by Aurora Riverboats Inc., a division of Dallas-based Pratt Hotels Corp., owner of the Sands hotel-casino complex in Atlantic City and the Sands in San Juan, Puerto Rico.
William Weidner, president of Pratt Hotels, said the $60 million Aurora project is expected to gross $100 million a year, including revenue from gaming, restaurants, entertainment and parking.
But every James Bond thriller has its archvillain, and to some riverboat gaming promoters the villain in this case is Mayor Richard Daley, who wants a $2 billion casino-entertainment complex built near downtown Chicago. It is projected that the 100- to 150-acre facility with three casino hotels would generate $370 million in local and state taxes its first year of operation in 1997.
''Mayor Daley wants his casino at the expense of the rest of the state,'' said Joliet`s Mezera. ''There`s no question that a land-based casino would be detrimental to river towns and riverboat gambling. A Chicago casino complex would saturate the market, because it would be the equivalent size of 20 riverboats.''
Aurora Ald. Mike Saville commented on the Chicago proposal: ''We feel the law allowing riverboat gambling was put in place to help the river towns. It`s not a good idea to change the rules in the middle of the game.''
The Rivertowns Coalition has been formed to oppose the Chicago plan. Spokesperson for the group last week before an Illinios Senate committe was Ruth Fitzgerald, president and CEO of the Will County Chamber of Commerce.
''The Chicago proposal is too much, too fast, too soon. We urge the legislature to oppose land-based casinos in Chicago and give the river towns a chance, as the law intended,'' she said.
However, two of the riverboat operators have taken a positive stance.
Instead of opposing land-based casinos in Chicago, Wilmott said Harrah`s supports it. ''We would welcome the opportunity to operate in Chicago. Because our riverboat will offer a different type of experience, we think it could co- exist (with land-based casinos).''
''We`re not opposed to gaming downtown,'' said Pratt Hotel`s Weidner. However, he acknowledged that the Chicago proposal put a temporary freeze on the Aurora project and was part of the reason for delaying its launching from this fall to next summer.
But no matter what happens in Chicago, it appears suburban riverboat gambling is steaming full speed ahead.
Larry Sak, president of the Joliet Region Chamber of Commerce and Industry, said that 6 of 10 calls to his office recently have been inquiries about riverboat gambling. ''The interest is there,'' he said.
The Empress is sure to benefit from being first on the water. Six daily trips will be offered, with tickets costing from $8 to $18, depending on the time and day. Cruises will head down river three to four miles and will last two hours, though gaming begins a half-hour before departure and continues a half-hour after return.
Certain to be ''winners'' every day are the 700 new Empress employees. The boat`s dealer`s school recently graduated 120 students.
''We look at the gaming boats as a marketing tool for Joliet,'' said Fisher. ''When 6,000 people a day come here (once both boats are operating), their negative perceptions of Joliet as a dirty, old industrial town will be changed.
''That could spur new housing sales on our west side, where we`ve platted over 2,000 lots for single-family homes. Many buyers are coming from Du Page and southwest Cook County because of our good values. Also, revitalization of the city center could spread out to older neighborhoods.''
Fisher stressed, though, that Joliet had planned to redevelop its downtown in 1990, before Illinois approved riverboat gambling.
A $14.5 million bond issue was passed in June, 1991, to fund such improvements as a riverwalk on the west bank, decorative lighting of two bridges, viaduct enhancements, neighborhood programs and construction of the gambling boat basin on the east side of the river.
Other ongoing improvements include a $7 million four-lane, tree-lined entryway into the city from Interstate Highway 80 and a $3 million downtown streetscape project.
According to Fisher, Joliet is considering splitting its gaming revenue 50-50 between neighborhood infrastructure rehab and downtown rehab.
Key elements of the downtown resurgence will be increased use of the historic Rialto Square Theatre (renovated in 1981) and new restaurants to be developed in the recently rehabbed Union Station.
Several facilities, including an auto dealership and a fire station, will have to be relocated to make way for the boat basin, which will be cut into the shore at the three-square-block gambling boat site.
Fisher said Harrah`s has agreed to fund land acquisition as well as pay $1.2 million to construct a new fire station and $1 million for riverwalks on the east bank.
Wilmott said construction already has begun on Harrah`s boat, ''a $15 million mega-yacht for 1,200 passengers. The 210-foot-long craft will be outfitted with 600 slot machines and 25 gaming tables, and is scheduled to make six daily cruises when it gets underway next April.''
He estimates the Joliet project will generate 300 construction jobs and 500 permanent jobs, though 50 to 70 of those positions will be filled by employees relocated from Harrah`s other casinos, which are in Las Vegas, Lake Tahoe, Atlantic City and Laughlin, Nev.
In contrast to the modern flair of the Joliet riverboats, Aurora has opted for the lure of yesteryear. Pratt Hotels` Aurora Riverboats Inc. will build two paddlewheelers, each capable of carrying 600 passengers and costing $7 million.
''Contracts have been let (for the boats), steel is on order, and work already has begun at North Point, where the boats will dock,'' said Ed Hanson, architectural director for Pratt Hotels.
The 145-foot-long craft will be built at the Garbe Iron Works in Aurora, and are scheduled to be completed by mid-June of 1993, according to Hanson. They will cruise between two bridges on the Fox River that are a mile apart.
In art-deco style, the boats` interiors will be loaded with 280 slots and 32 gaming tables, but no restaurants. Hungry gamblers will be catered to in the four-level, 64,000-square-foot docking pavilion on the tip of Stolp Island.
The historic Paramount Arts Centre, about a block from the dock, also is expected to help attract visitors because it will feature big-name acts, according to Weidner of Pratt Hotels.
''We will have some of the same acts in Aurora as we have at the Sands in Atlantic City. We`re negotiating for mega-acts, including Sinatra, Cher and Liza (Minnelli),'' Weidner said.
The 1,888-seat Paramount was built in 1931 as a premier movie palace of that era.
Included in each boat ticket is a $2 tax, $1 of which goes to the State of Illinois and $1 to the community. Aurora is considering giving a third of each dollar to the theater, owned by the Aurora Civic Center Authority; a third to the Riverwalk Commission; and a third to neighborhood programs.
Besides the construction of the docking pavilion on the north end of Stolp Island, the redevelopment will include $2 million of riverwalks (funded by Pratt), a canoe chute and an $8 million, 850-car parking garage.
The three-tier riverwalk will include the street level, a midlevel with shops and restaurants and lower level for walking and biking.
Weidner said Pratt might be interested in rehabbing the old Aurora Hotel, a vacant eight-story structure near the gaming center that is not included in the first phase of development. The city recently purchased the 100-room hotel for $120,000.
Paul Borek, executive director of the Aurora Economic Development Commission, anticipates that riverboat gambling will create 800 to 1,000 new jobs in Aurora.
Mayor Pierce of Aurora said that studies have shown that the Chicago area could support 8 to 10 riverboats.
So what are the odds that riverboat gambling will strike it rich in Chicago`s suburbs? Several experts think the outlook is good, pointing to the Par-A-Dice, the 1,000-passenger sternwheeler that opened in Peoria last November and has exceeded expectations.
If it can play in Peoria, why not in Joliet and Aurora?


text:
Welcome to this STUNNING 4-bedroom, 2-bathroom home that boasts an open-concept layout, perfect for modern living. As you enter the home, you'll immediately notice the beautiful California-style kitchen island, perfect for entertaining guests or enjoying a quiet meal at home. The stone fireplace in the living room adds a touch of warmth and elegance to the space, creating a cozy ambiance for all to enjoy.
The split bedroom concept allows for maximum privacy, with the master bedroom located on one side of the home and the additional bedrooms located on the other. The master suite is truly a luxurious retreat, featuring a beautiful ensuite and a large walk-in closet that conveniently connects with the great utility room.
This home is perfect for those who love to entertain or simply enjoy the comfort of a well-designed space. Don't miss out on the opportunity to make this beautiful property your very own!
Properties marked by the NTREIS Logo are courtesy of the NTREIS Multiple Listing Service. Property listing data provided by NTREIS multiple listing service. You may not reproduce or redistribute this data, it is for viewing purposes only. This data is deemed reliable, but is not guaranteed accurate by the MLS or NTREIS. Updated: 3rd October, 2023 10:27 AM (UTC)

domain: design


text: Meet Brandon and Amanda; two of the kindest people you will ever meet. These two have been dear friends of Erik and I for a long time, and I am so thankful we had the opportunity to get together for this shoot. I’m not lying when I say that these images absolutely captivate me. They are some of my most favorite; ever. Isn’t Amanda stunning? Like, truly, stunning. And Brandon and his smooth style…incredible. Thank you two for being creative along side of me, and allowing me to really capture who you are. I am over-the-moon excited to share these images with you!

domain: photo


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A rapid, water-soluble enzyme-mediated radical chain initiation system involving glucose oxidase and Fe+2 generated hydrogels within minutes at 25°C and in ambient oxygen. The initiation components were evaluated for their effect on polymerization rates of hydroxyethyl acrylate-poly(ethylene glycol)575 diacrylate comonomer solutions using near-infrared spectroscopy. Increasing glucose concentration increased polymerization rates until reaching a rate plateau above 1 × 10−3M of glucose. A square root dependence of the initial polymerization rate on Fe+2 concentration was observed between 1.0 × 10−4M and 5.0 × 10−4M of Fe+2 whereupon excess Fe+2 reduced final acrylate conversions. The glucose oxidase-mediated initiation system was employed for encapsulation of fibroblasts (NIH3T3s) into a poly(ethylene glycol) tetra-acrylate (Mn~20,000) hydrogel scaffold demonstrating 96% (±3%) viability at 24 hours post-encapsulation. This first use of enzyme-mediated redox radical chain initiation for cellular encapsulation demonstrates polymerization of hydrogels in situ with kinetic control, minimal oxygen inhibition issues and utilization of low initiator concentrations.
A hydrophilic and crosslinked hydrogel, that prevents dissolution while promoting water uptake, facilitates biomedical application including drug delivery, biosensor fabrication and tissue engineering.1 One prominent tissue regenerative approach encapsulates cells into supportive three-dimensional hydrogel scaffolds to guide cellular adhesion, molecular signaling, proliferation and, ultimately, tissue formation.2–4 The homogenous distribution of cells and biomolecules in the hydrogel precursor solution permits the delivery to any targeted site, allowing morphologic customization of the polymerized gel. A realization of such hydrogel cellular scaffolds requires a gelation process wherein the precursor solution is water-soluble; the polymerization conditions are mild; the cure time is readily tailored for a particular application; and the gel components are all cytocompatible.
The use of radical chain polymerization of high molecular weight poly(ethylene glycol) (PEG) vinyl monomers (i.e., macromers) often meets these demands and further permits molecular design of mechanical properties, swelling behavior and bioactive molecule incorporation. Although photoinitiation is frequently employed for generation of these cell-laden scaffolds,5–8 photopolymerization limitations associated with cure depth and shadow effects have prompted investigations into successful light independent encapsulation systems using the thermally activated ammonium persulfate-tetramethylethylenediamine (APS/TEMED) initiation.9–11 Additionally, utilizing ascorbic acid in combination with persulfate ions, Mikos and coworkers described the significant advantages of redox initiation pathways for generating hydrogels and the importance of the redox initiator components on cellular environments.12 Another redox initiation system involving Fenton’s reagent,13,14 comprising a ferrous salt (Fe+2) and hydrogen peroxide (H2O2), was successfully employed for the polymerization of poly(vinyl alcohol) (PVA) hydrogels showing unique and advantageous drug release profiles compared with UV cured PVA hydrogels.15 However, this rapid Fenton system also demonstrates unique challenges in finely controlling cure times and avoiding the formation of microgel domains.16
Other light-independent initiation systems include enzymes that catalyze electron transfer reactions and subsequently generate products capable of initiating chain polymerization of water-soluble vinyl monomers; these include certain oxidoreductase catalytic mechanisms which directly form a primary radical appropriate for initiating the vinyl polymerization reaction. An eminent example involves the horseradish peroxidase (HRP) enzyme that catalyzes the oxidation of 2,4-pentanedione by H2O2 generating a radical initiating species on the acetylacetone molecule.17 Although this HRP ternary system demonstrates successful polymerization of monomers including acrylamide and hydroxyethylmethacrylate,18,19 the scheme also shows extended and irregular inhibitory periods of between 45 and100 minutes.20 Another ternary system consisting of manganese peroxidase, H2O2 and 2,4-pentanedione initiated the polymerization of acrylamide but required 12 hours under a nitrogen atmosphere.21 Such extended polymerization times do not facilitate the rapid formation of hydrogels as needed in cellular encapsulation. To date, no enzyme-mediated chain initiation systems have been employed for cellular encapsulation into hydrogel scaffolds.
Enzyme-mediated initiation systems involving oxidase enzymes that produce H2O2, including the glucose oxidase (GOX) enzyme, offers a beneficial approach for rapidly polymerizing hydrogels. In the GOX initiation system, the enzyme binds to the glucose substrate generating gluconolactone and subsequently regenerates the flavin adenine dinucleotide (FAD) cofactor by binding oxygen and producing H2O2. By coupling ferrous ions to this enzymatic process, hydroxyl radical species are produced. Notably, a unique aspect of this system involves the utilization and elimination of oxygen, a powerful inhibitor of radical chain polymerization, by the GOX enzyme during the initiation process. This feature offers unique advantages, including the ability to reduce initiator quantities and eliminate the requirement of performing reactions under inert atmospheric conditions which precludes the ability to encapsulate cells. For example, the GOX initiation system was actually completely suppressed under a nitrogen atmosphere until the introduction of oxygen whereupon polymerization quickly commenced.22 This compatibility with oxygen further prompted the use of this GOX system for the polymerization of detachable balloon catheters used in endovascular surgery where oxygen flow to blood and tissue is critical.23 The tolerance of the GOX system to oxygen and the elimination of an extraneous energy source for initiation, such as light or heat, prompted our investigations to understand further the polymerization kinetics and employ this system for cellular encapsulation.
Herein, we detail the chain polymerization kinetic reactions and mechanism of the GOX mediated initiation system for forming polymers with biomaterials applications. The outlined kinetic parameters provide a simple means to control the final vinyl conversion and tailor the polymerization rates. The understanding of these kinetic reactions facilitated the encapsulation of mammalian cells with high viability.
The polymerization reactions were monitored using Fourier transform infrared (FTIR) spectroscopy with a Nicolet 750 Magna FTIR instrument. The acrylate double bond conversion was followed in real time using the near-IR absorption peak between 6212–6150 cm−1 corresponding to the C-H stretch of the acrylate functional group. The polymerization reaction commenced with the addition of GOX from Aspergillus niger (Sigma-Aldrich) to a mixing solution of all other reaction components comprising iron (II) sulfate (Fe+2) (Sigma-Aldrich), glucose (Sigma-Aldrich), 2-hydroxyethyl acrylate (HEA), poly(ethylene glycol) diacrylate (Mn~575 Da) (Sigma-Aldrich) and 2-(N-morpholino)ethanesulfonic acid (MES) buffer stabilized at pH=4.5 (Teknova). The MES buffer was chosen for these kinetic studies to maintain slightly acidic conditions favorable for the GOX enzyme. A 10% (w/v) glucose stock solution was stabilized to ensure mutarotation of the sugar. After mixing, the reactions were immediately transferred to a 1mm thick glass sample compartment and placed in the FTIR instrument that utilized a horizontal transmission apparatus.24 All the reactions were performed sealed from the open atmosphere at ambient temperature without a solution purge of oxygen. Negative controls were performed by eliminating either GOX, glucose or Fe+2 from the reaction mixture and each resulted in no polymerization after 30 minutes. A slight induction period was partly attributed to the presence of the hydroquinone monomethylether (MEHQ) inhibitor in the unpurified commercial monomers. This induction period permitted the acquisition of a reliable zero conversion baseline utilized in the data analysis. The initial polymerization rates (Rp) were obtained by determining the time required to react from 15% to 30% double bond conversion and each experimental condition was performed three times. Rheological measurements using an ARES rheometer (TA Instruments) were employed to verify the polymerization of the poly(ethylene glycol) tetra-acrylate (Mn~20000 Da). For rheological measurements, the reaction materials were mixed in the same manner used for the near-IR experiments and promptly sandwiched between 20mm plates in parallel configuration.
The NIH3T3 fibroblast cells were cultured using Dulbecco’s modified eagle medium (DMEM) containing 25mM glucose (Gibco) and further supplemented with 10% fetal bovine serum (FBS), 1µg/mL amphotericin, 50U/mL penicillin, 50ug/mL streptomycin and 20ug/mL gentamicin. The cells were cultured under standard conditions (37°C and 5% CO2) both prior to and following encapsulation
The poly(ethylene glycol) tetra-acrylate (Mn~20000) (PEGTA20000) was synthesized according to previously published protocols.25 The characterization of the PEGTA20000 product with H-NMR confirmed 95% acrylation. A peptide comprising CRGDS (cysteine, arginine, glycine, aspartic acid, serine) was synthesized using a 433A peptide synthesizer (Applied Biosystems) and purified by reverse phase high performance liquid chromatography. The identity of the peptide was verified by MALDI-MS. An Ellman’s colorimetric assay26 was also used to verify and quantify the presence of reduced thiols on cysteine residues, which permit the facile incorporation of the peptide within the hydrogel network.27 Briefly, the cysteine residues present on the CRGDS peptide are incorporated into the hydrogel by chain transfer during the homopolymerization of the acrylate groups. Inclusion of this peptide in the hydrogel facilitates cellular adhesion and survival in synthetic hydrogels.28
Fibroblasts were encapsulated into hydrogels at a density of 30 × 106 cells/mL, by gently suspending the cells in a PEGTA20000 monomer formulation to obtain a final concentration of 2.5 × 10−5 M GOX, 1.25mM Fe+2, 4mM glucose, 15wt% PEGTA20000, and 1mM of CRGDS in a 1X Dulbecco’s Phosphate Buffered Saline (PBS) solution (pH=7.2–7.4). The mixture was added to a cylindrical mold (4mm diameter, 1.5mm height) and permitted to polymerize for approximately 5 minutes. The gels were incubated for 30 minutes in 1X PBS (pH=7.2–7.4) at 37°C before transferring the gels to the appropriate media or cell viability solution. For experiments involving the catalase enzyme, the gels were incubated under standard conditions in DMEM cell culture media containing a final catalase concentration of 2.0 × 10−6 M for 24 hours. The catalase enzyme catalyzes the degradation of H2O2 to water and O2, both products well tolerated by cells. Cell viability was determined using the Live/Dead cellular stain (Invitrogen), a membrane integrity assay that stains living cells green and dead cells red. The fluorescence images were obtained using confocal microscopy, 3 images taken at random positions for each gel examined. Live, green cells were counted using MetaMorph software; dead, red cells were counted manually.
The GOX enzyme catalyzes the oxidation of glucose to gluconolactone and subsequently regenerates the FAD cofactor by reducing O2 to H2O2 (Equation 1). By combining the enzymatically produced H2O2 with ferrous ions, a hydroxyl radical initiator is generated (Equation 2) which may further react with vinyl monomer [M] to produce a chain initiating species (Equation 4) or react with additional ferrous ion to inhibit polymerization (Equation 3). Overall, the present studies found that this GOX-mediated initiation system promotes rapid polymerization rates reaching high acrylic double bond conversion, usually within about three minutes under current conditions at ambient temperature and atmosphere. Moreover, the polymerization rates were easily tailored using a HEA-PEGDA575 monomer formulation by changing the initiator component concentrations (i.e., glucose, GOX enzyme, Fe+2). Detailed investigations of polymerization kinetics were required to understand further this initiation system for use in cellular encapsulation studies.
Studies were performed to investigate the effects of H2O2 generation (Equation 1) on the polymerization kinetics, including the effects of glucose and GOX concentrations on polymerization rates. First, the effects of glucose on polymerization rates were evaluated by monitoring ambient temperature polymerization reactions at differing glucose concentrations using near-IR spectroscopy. Representative acrylate conversion profiles for various glucose concentrations using 6.25 × 10−7 M GOX enzyme and 2.5 × 10−4 M Fe+2 (Figure 1A) display the general trend that the increase in glucose concentrations results in an increase in polymerization rates when all other reaction components are maintained constant. This trend would be expected since increasing the glucose substrate results in the increase in the H2O2 generation rate by the GOX enzyme.
These acrylate conversion profiles were further used to determine scaling relationships between the initial polymerization rates (Rp) and glucose concentrations at different fixed ferrous values (Figure 1B). Since the polymerization rate scales with initiation rate31, direct monitoring of the polymerization reaction (i.e., double bond conversion) permits a further understanding of the initiation reaction and ultimately its mechanisms. Classically, assuming pseudosteady state and chain length independent termination, the radical chain polymerization rate is proportional to the square root of the initiation rate (Ri) indicating bimolecular termination (Rp ∞ Riα, where α = 0.5).31,32 A divergence of the scaling exponent (α) from 0.5 is attributed to alterations in the typical bimolecular termination mode, including α < 0.5 for systems involving chain length dependent termination33 and α > 0.5 for systems encountering inhibitory or unimolecular termination.34 Here, the dependence of polymerization rates on glucose concentrations displayed a dynamic variation of scaling exponents changing from α = 1, at lower glucose values, to α = 0.5 at increased glucose values. At α = 1, a first order dependence of polymerization rate on glucose concentration indicates a deviation from bimolecular termination likely associated with an inhibitory termination reaction. Evidence for such an inhibitory reaction is also suggested by the conversion profiles in Figure 1A where at lower glucose value (i.e., below 1.75 × 10−4 M of glucose), the reactions do not achieve complete double bond conversion. This inhibitory reaction is further evaluated and discussed in the following section concerning iron and polymerization.
Interestingly, at higher glucose concentrations, approximately 1.0 × 10−3 M and above, the initial polymerization rates do not change as the glucose concentration changes and ultimately maintain a zero order dependence with respect to glucose. This behavior implies that as the polymerization rate saturates, another component of the initiation system becomes limiting (i.e., Fe+2, GOX, O2). Further tests were performed by monitoring polymerization reactions at various GOX enzyme concentrations (Figure 2). In Figure 2, all reactions contain identical [Fe+2] at 2.0 × 10−4M, whereas each line represents a unique and fixed glucose value. Each line displays a near square root dependence of the polymerization rate on the enzyme concentration until a saturation point is achieved, after which the polymerization rate maintains a zero order dependence on GOX. Notably, the saturation point is reached at, and above, 6.25 × 10−7 M of GOX enzyme, the same concentration of enzyme employed in Figure 1. This result indicates that a point is reached whereupon additional glucose or GOX enzyme does not increase the polymerization rate.
Taken together, the saturation rate behavior achieved at high concentrations of glucose (Figure 1B) and high concentrations of GOX (Figure 2) is partly explained by understanding the enzymatic H2O2 production mechanism. In this mechanism, increased glucose utilization is accompanied by increased oxygen consumption due to the enzymatic regeneration of the FAD cofactor. Before the polymerization reaction begins, the total dissolved oxygen concentration in these GOX reactions should approximate that of the dissolved oxygen found in a typical acrylate formulation (~1.0 × 10−3M) since the reactions were sealed without an initial solution purge of oxygen.34 The zero order dependence of polymerization rates implies that enzymatic consumption of oxygen contributes to the polymerization rate plateau observed at these higher glucose and GOX levels. Additionally, although the iron species is catalytic in this system (see below discussion concerning iron), the consumption of Fe+2 ferrous may also contribute to this rate plateau behavior at high glucose and GOX concentrations.
The quantity of iron present in a GOX-mediated initiation reaction is crucial since Fe+2 can either promote polymerization by forming the hydroxyl primary radical (Equation 2) or hinder polymerization by destroying the same hydroxyl radical, thus limiting the chain initiation events (Equation 3). Understanding the effects of iron on final double bond conversion and polymerization rates is essential for using the GOX initiation system for biomedical applications such as cellular encapsulation. Incomplete functional group conversion could inhibit the formation of mechanically stable and fully crosslinked hydrogel scaffolds or could result in cell viability loss due to the presence of unreacted monomer. Moreover, understanding the kinetic effects of iron is essential for tuning cure times.
The effect of the [Fe+2] on polymerization kinetics was monitored using near-IR spectroscopy by varying the ferrous concentrations while maintaining identical concentrations of all remaining initiation components (i.e., glucose, GOX, monomer). An increase of initial Rp values with an increase in Fe+2 concentration was expected for a certain range of iron and confirmed as shown in Figure 3. The results in Figure 3 also illustrate that an increase in fixed glucose concentrations (each line represents a unique and fixed glucose value) increases the overall Rp values while maintaining the same scaling exponents (α) values at 0.5 (Table 1). This polymerization rate dependence on the square root of the ferrous ion concentration indicates typical bimolecular termination with negligible inhibitory reactions within this range of Fe+2 values. Moreover, when varying Fe+2 concentrations, the polymerization rates overlap for 1.0 × 10−3 M and 1.5 × 10−3M of fixed glucose amounts (Figure 3). This behavior is consistent with the zero order dependence on glucose observed above 1.0 × 10−3 M as discussed in the previous section. However, at lower glucose and Fe+2 values, the Rp rapidly and significantly drops as shown for 3.0 × 10−4 M and 1.75 × 10−4 M of glucose. This drop in Rp is attributed to the low concentration of hydroxyl initiating species which are unable to overcome termination reactions.
To understand the termination mechanism further, the final double bond conversions were examined. As shown in Figure 4A, a considerable decrease in final conversion occurs with an increase in [Fe+2], suggesting an inhibitory reaction. The increase in Fe+2 accompanies the radical wasting reaction (Equation 3) and likely promotes the decrease in double bond conversion as previously reported.23 However, in addition to this wasting reaction, certain metal salts, including Fe+3, may terminate propagating radical chains by oxidizing the growing polymer chain radical (Mn•) through an electron transfer process as shown in Equation 5.35
Since ferric ions are generated during the initiation reaction (Equations 2 and 3), it was anticipated that the conversion decrease was partially attributed to the accumulation of Fe+3. However, all monomers are not similarly affected by Fe+3 as shown by the resistance of poly(methyl methacrylate) radicals to ferric termination attributed to steric influence.35 To test the susceptibility of the current monomer formulation to ferric termination, the GOX mediated polymerization reaction with the HEA-PEGDA575 formulation and 1.0 × 10−3 M glucose was performed with supplemented ferric ions (Figure 4B). Significant drops in conversion occurred with increasing Fe+3, indicating that these inhibitors cause premature chain termination in this system. For example, an average decrease in final conversion of 9%(± 3%), 30%(± 5%) and 59%(± 4%) occurred with the addition of 5.0 × 10−4 M, 7.5 × 10−4 M and 1.0 × 10−3 M of Fe+3, respectively.
Importantly, Figure 4A also displays that the reactions become more susceptible to iron inhibition as the glucose concentration used to initiate the reaction is reduced. For example, 95% (± 0.3%) conversion was achieved with reactions containing 5 × 10−4 M Fe+2 and 1.0 × 10−3 M glucose whereas only 74% (± 2%) conversion was reached with reactions containing 5 × 10−4 M Fe+2 and 1.75 × 10−4 M glucose. This result would indicate that, in general, maintaining a high glucose:iron concentration ratio may largely eliminate iron’s inhibitory reactions and promote polymerization. Interestingly, the results in Figure 4A nearly collapse onto a single line when re-plotted as the mole ratio of (Fe+2)2/(glucose) versus final double bond conversion (Figure 5). This plot displays the trend that as the mole ratio of (Fe+2)2/(glucose) becomes smaller (i.e., reaching 10−7), the final double bond conversion approaches 100%. This again emphasizes the benefits of maintaining a high glucose: iron ratio.
The capacity for high conversion with trace ferrous iron is explained by understanding the catalytic nature of iron in this initiation system. As shown in Equation 5, the termination of propagating chains by Fe+3 regenerates Fe+2 which can subsequently interact with another H2O2 molecule to initiate polymerization. Since the generation of H2O2 scales with the quantity of glucose present in the GOX system, providing excess glucose promotes the formation of the hydroxyl radical from the catalytic iron. These findings are in agreement with the importance of the stoichiometric relationship between the components of Fenton’s reagent, namely the [Fe+2], [Fe+3] and [H2O2].36 For example, an optimized molar ratio of [H2O2]/[Fe+2] = 10 was found when using only Fenton’s reagent for the production of poly(N-vinyl-2-pyrrolidone).14
Cellular encapsulation in hydrogels requires biocompatible, crosslinked and high molecular weight macromers (e.g., acrylated PEGs), typically with a molecular weight greater than 3kDa, to ensure diffusion of bioactive molecules between the cellular hydrogel and the surrounding media.37 Here, we employed PEGTA20000 (Figure 6A) to encapsulate fibroblasts into a hydrogel using the GOX-mediated polymerization reaction. Monitoring the polymerization kinetics of PEGTA20000 with IR spectroscopy proved infeasible due to the relatively insignificant absorption of the carbon-carbon double bond of the acrylate group. However, the characteristics of the GOX-mediated initiation reaction, as revealed by the HEA-PEGDA575 polymerization studies, should remain unaffected despite the slightly different monomer formulation (i.e., PEGTA20000). For the encapsulation reactions during the polymerization of PEGTA20000, the glucose concentration was maintained in excess of the ferrous ion concentrations, consistent with Figure 4, to eliminate excessive radical termination reactions with excess iron that would potentially result in incomplete acrylate conversion. Moreover, since the polymerization rates are also dependent on monomer concentration,31 tailoring the GOX initiation reaction for the polymerization using the 15wt% PEGTA20000 monomer formulation (that contains a lower concentration of acrylate groups and a neutral pH) required employing an overall higher concentration of glucose and iron to achieve gelation within an appropriate time period. The polymerization kinetics are not expected to be greatly affected from the CRGDS peptide (present at 1mM) as the propagation reaction is dominated by the chain growth mechanism associated with the excess of PEGTA20000 acrylate (~30mM acrylate concentration). The polymerization of a 15wt% PEGTA20000 monomer solution using 1mM CRGDS peptide, 4.0 × 10−3 M glucose, 1.25 × 10−3 M Fe+2 and 2.5 × 10−5 M of GOX enzyme was monitored using rheometry, obtaining a final modulus of 10kPA within three minutes.
For the cellular encapsulation reactions, immediately upon combining all GOX initiation components, the cells were gently mixed with this precursor solution, transferred to cylindrical molds and permitted to polymerize. The GOX initiation system rapidly cured (~ 3 minutes) into a cylindrical shape hydrogel (4mm diameter × 1.5mm height) using a 1X solution of PBS containing 1mM CRGDS peptide, 4.0 × 10−3 M glucose, 1.25 × 10−3 M Fe+2, 15wt% PEDTA20000, and 2.5 × 10−5 M GOX. It would be expected that post-polymerization hydrogels will contain residual initiator components including the GOX enzyme as the diffusion of proteins out of high molecular weight PEG gels requires days.38
To investigate the effects of the remaining encapsulated initiator constituents on cell viability, gels were incubated in cell media for 0, 1 and 24 hours post-polymerization. The hydrogels were swollen in DMEM media containing 25mM of glucose. Thus, the cells are subject to H2O2 production from any remaining active GOX enzyme. However, cell viability was 98% (±1%) for incubation in media not containing catalase for one hour and 96% (±3%) for incubation in media supplemented with 2.0 × 10−6 M of catalase for 24 hours (Figure 6B). Since the catalase enzyme catalyzes the decomposition of H2O2 to water and oxygen, it would be expected that the presence of catalase post-polymerization would assist in eliminating potential H2O2 generated from residual and active GOX enzyme. The gels incubated in media lacking catalase displayed lower viability (87% ±3%), suggesting that the catalase enzyme may provide protection for cells encapsulated using GOX-mediated initiation by eliminating excess H2O2 generated by the GOX enzyme. Overall, the cells tolerate the GOX-mediated initiation reaction for cellular encapsulation (independent of catalase) as demonstrated by the high viability after one hour. Furthermore, the cellular viability is enhanced at 24 hours post-encapsulation by including the catalase enzyme in the cell media.
Due to the recognized cytotoxicity of H2O2, the high viability of cells encapsulated with the GOX mediated system was unanticipated. However, this result does agree with prior studies demonstrating that the presence of poly(vinyl alcohol) (PVA) polymer significantly decreases the cytotoxicity of murine fibroblasts exposed to H2O2.16 In this study with PVA, the presence of the hydrogel material was suggested to provide cellular protection against the hydroxyl radical. An additional report using oxidative polymerization of phenol by the horseradish peroxidase enzyme that exposed cells to H2O2 during cellular encapsulation into alginate-phenol gels also demonstrated high cellular viability.39 Altogether, these investigations imply that polymeric hydrogel materials may provide protection against the H2O2 during initiation and against potential residual oxidant species generated from any remaining active GOX enzyme within the hydrogel scaffold. Additional investigations would be necessary to understand further the potential protective qualities of the hydrogel material with respect to the GOX initiation system.
Overall, these results demonstrate the first use of the GOX-mediated radical initiation system for the generation of biocompatible hydrogels used to encapsulate fibroblasts with high cellular viability. In these studies, the GOX-mediated cellular encapsulation was performed at ambient temperature. However, this reaction would also be expected to perform well, and with potentially increased polymerization rates, at physiological temperature (i.e., 37°C) as GOX remains stable and active at this temperature. It is also anticipated that the GOX system would not encounter iron inhibitory termination reactions (as discussed in Figure 4) from endogenous iron in biological samples such as serum. Under normal physiological conditions, extracellular iron pools are sequestered by iron binding proteins (e.g., ferritin) or chelating agents and are present at lower concentrations than the current Fe+2 initiation component. As with any new material, this initiating system would require further understanding molecular release from hydrogels (e.g., the GOX enzyme) and general biocompatibility for future in vivo applications. However, the utilization of the GOX-enzyme in implantable devices such as the commercially available, subcutaneous glucose sensor40 is encouraging for the overall biological tolerance of GOX-containing materials.
Given the rapid and predictable nature of the initiation, the GOX system may offer benefits for additional biomedical applications. For instance, GOX immobilization in hydrogels is highly significant for producing glucose responsive hydrogel biosensors to mimic islet cell activity for the treatment and monitoring of diabetes.41 Current techniques for GOX immobilization in a hydrogel involve employing other radical chain initiators in the presence of GOX,42,43 or swelling hydrogels in a GOX solution.44 In the current work, the GOX enzyme is encapsulated within the hydrogel during the initiation process and, in effect, immobilizes itself into the polymer matrix. By using GOX to generate the H2O2 initiator, a facile approach for fabricating GOX immobilized glucose hydrogels for sensor applications may be achieved.
The benefits and mechanisms of the GOX-mediated initiation reaction are demonstrated by monitoring a series of polymerization reactions using near-IR spectroscopy with a HEA-PEGDA575 monomer formulation. Utilizing this formulation, we demonstrated that the GOX-mediated initiation system provides a rapid and tunable approach for generating hydrogels. The polymerization occurs within minutes at ambient temperature without requiring any oxygen purge and using low concentrations of initiation components. Moreover, this system uniquely overcomes potential oxygen inhibition by using the GOX enzyme to locally consume oxygen and subsequently generate the H2O2 initiator. The polymerization rates increase with increasing glucose until, ultimately, achieving zero order dependence above 1 × 10−3 M glucose. Additionally, the initiation reaction shows susceptibility to inhibitory reactions from an excess of iron and this behavior is avoided by using a high ratio of glucose: iron.
The GOX initiation system was employed for cellular encapsulation of a fibroblast cell line (NIH3T3s) into a 15wt% PEGTA20000 hydrogel scaffold. The decrease in acrylate concentration using this PEGTA20000 monomer formulation contributed to the requirement to increase the initiation components while still maintaining a high glucose: iron mole ratio. At 24 hours post-encapsulation, the cells demonstrate high viability indicating that residual initiation components have minimal deleterious effects. This system is the first demonstration of using an enzyme-mediated chain initiation reaction for cellular encapsulation and offers an alternative light-independent approach for generating hydrogel scaffolds to support cellular growth. This elimination of light dependence may provide additional benefits including the capability to cure intricate shapes without concerns associated with shadowing effects and the potential capacity to perform in clinical applications that are limited by light penetration.
This work has been supported by the State of Colorado and the University of Colorado Technology Transfer Office, by National Institutes of Health Grant No. R21 CA 127884, and by NSF grant CBET 0626023. L.M.J. and B.D.F. acknowledge the Graduate Assistantship in Areas of National Need Fellowship from the U.S. Department of Education.
Supporting Information Available. The supplemental data includes fluorescent images of cell-laden gels post-encapsulation with the GOX-mediated initiation system. These images display cells after incubation in glucose containing media for differing time points. This material is available free of charge via the Internet at http://pubs.acs.org.

domain: chemistry


General Nikolai Makarov, chief of the General Staff, said Tuesday that the armed forces will fully abandon analog infrastructure by 2012 and spend 300 billion rubles ($10 billion) to create new digital systems.
“Creating the foundation for a new system of direction for our army,” he said, “is one of our fundamental tasks,” Interfax reported.
Makarov made the announcement as part of a whole host of clarifications on Russia's military goals from 2011 to 2020.
Prime Minister Vladimir Putin said Monday that the government has earmarked 20 trillion rubles ($646 billion) to rearm and modernize the country's military over the next nine years.
Deputy Prime Minister Sergei Ivanov said 79 percent of the Defense Ministry's budget will be spent on the purchase of high-tech weapons. The rest will be spent on modernizing and repairing existing equipment.
A new air-defense system will be a cornerstone of the long-term plan. Makarov said the foundation for a unified air defense system will be laid in 2012 and “functioning at full capacity from 2020.”
At present, different branches of the country's military are responsible for different aspects of missile defense.
“We want to unite all the disparate elements into a single, integrated system under a unified command,” Makarov said, “so that the state can exist under an ‘umbrella’ protecting it from strikes by intercontinental ballistic missiles, intermediate-range missiles, cruise missiles, space, naval and ground missiles … even at a low altitude, at any time and in any circumstance.”
“This is a comparatively lengthy and demanding process requiring significant financial investment,” he added.
First Deputy Defense Minister, Vladimir Popovkin, said Monday that Russia's current priorities for military procurement were “strategic missile forces, missile defense, high-precision weapons, the management system for the country's strategic reserve and electronic warfare,” Bloomberg reported.
In his speech to a meeting on Monday addressing Russia's military spending through 2020, Putin highlighted the technological innovations that these priority areas will require. The prime minister said 1,300 new types of equipment will have to be purchased.
Seventeen percent of these will require “opening new or expanding old production facilities,” Putin said.
Building on a recent trend, some foreign equipment and technology will also be needed for the ambitious rearmament program. Makarov confirmed that a final decision on the purchase of French Mistral-class helicopter carriers, estimated to cost 6 million euros ($8 million) each, had been made and the tender's winner would be announced by year-end.
Defense Minister Anatoly Serdyukov announced Dec. 3 that Russia would buy 10 multifunctional armored personnel carriers from Italian company Iveco. Russia bought 12 unmanned drones from Israel in 2009 for $100 million.
Makarov also said the Russian military would come to rely more and more on contract soldiers. Changes would not happen “overnight” he said, but “we are aiming toward a fully contractual army.”
Putin said Monday that of the 20 trillion dollar budget for the next nine years, 4.7 trillion rubles will go to the navy.
This will not include the construction of any aircraft carriers, Ivanov said, but does cover a new generation of nuclear submarines.
Putin oversaw the first dockside trials on Monday for the new Borei-class submarine, Alexander Nevsky, at Severodvinsk on the White Sea.


|We're being updated from XP to Windows7 and one of my users is now unable to open an Excel WB that he created. Prior to his update, it opened in Excel 2010 but now, after a third user opened it at home on his company laptop, it's now a 97-2003 version. I know, this probably sounds confusing with the different people so bear with me:|
Mike, user 1, created an Excel2010 workbook while his OS was Windows XP, and viewed it for weeks as 2010.
Brad, user 2, accessed the WB from his company laptop, OS Windows7, but saved it as Excel 97-2003.
Mike gets Windows7 and can only open the WB as 97-2003 in Protection View with no way to take it out of that view to make changes.
Me, user 3, still on Windows XP, can open it as 97-2003 and can change it but not save it as 2010. If I try to open it in 2010, I get the 'Office has detected a problem and cannot open this file' error. If I open 2003, then I can open and change values in the spreadsheet but not the type and when I try, I get the same 'office has detected a problem' error.
What do you think? Is Excel 97-2003 incompatible with Windows7? did saving it that way mess it up? Why can't I change it back? And why is it in protected mode for the creator?
Suggestions are appreciated!


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domain: finance


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Tag Archives: judy reyes off the map
Having watched many many episodes from my favourite TV genre, it has come to my attention that certain actors undoubtedly have more experience in the medical field than others. Even big characters have been known to appear in more than … Continue reading


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Where to Find GCR Tires Near Scandia
We keep fleets and equipment moving where you need them to go. It's a job we've been striving to perfect since 1945, when the first GCR store opened in Odessa, Texas. Since then, GCR has become a leading provider of 24-hour road service and commercial tire services near Scandia, MN. Customers choose us for high-quality brands including Bandag, Bridgestone, Firestone, and Dayton products, plus premier customer service and advanced workmanship day after day. We make it happen thanks to our commercial tire stores and many qualified teammates. Call GCR Tires & Service near Scandia for responsive commercial tire solutions that support your fleet—in the field or on the road.
Turn to GCR Near Scandia for Your Commercial Tire Needs
You want affordable tires, whether you're trucking oranges from orchard to market, hauling coal from the Northeast, or delivering local construction materials. Get the right commercial tires at GCR Tires & Service near Scandia. Our tire options include mining, forklift, off-the-road, construction, logger, tractor, swamp buggy, skidder, and even camper tires— all in a variety of sizes and from commercial tire brands you trust most! We also operate ISO-certified Bandag Retread shops across the United States for fleet owners and operators looking for the best value in retreads.
Are you shopping for semi-truck tires near Scandia? We have the selection and experience to rig all of the wheel positions on your trailer and truck. Trailer tires, drive tires, steer tires —whatever the position, our teammates can help you decide on the best semi-truck tires for your fleet. Your local GCR Tires near Scandia is here to keep your truck rolling with the right commercial truck tires for the load, condition, and distance.
Don't forget about our affordable OTR tires for mining, construction, waste transportation, forestry, and more! All types of equipment operators trust commercial Firestone and Bridgestone OTR tires. These OTR tire brands are known for handling strenuous loads and conditions with ease.
But our experience doesn't stop when the pavement stops. You'll find our tractor, farm, and ag tires hard at work in North America's crop and ranchland, too. Our extensive selection of affordable farm tires near Scandia means we can keep all of your equipment moving, including combines, tractors, skid steer loaders, and sprayers. We also offer farm tire financing, tractor tire repair, and maintenance, so you don’t have to postpone a harvest. When you need farm tire repair, just call one of our service trucks. We'll come to you.
At GCR Tires & Service, we go where you go. Even underground with mining tires and tire monitoring services. Your job site is our destination. We'll be there with some of the best mining tire solutions to help you maximize output. Turn to GCR for all of your around the wheel needs, like Bridgestone mining tires, but also Bel-Ray lubricants, conveyor belts, hose solutions, flat-proofing, and torque wrenches near Scandia.
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When you're shopping for some of the best commercial tires, not many tire companies can compare to GCR's variety of services and tires. Got an SUV that needs winter tires? A flatbed in need of new drive tires? The best tires for a tractor? Browse tires online and visit GCR Tires & Service near Scandia.
Get Repairs & 24-Hour Truck Road Service Near Scandia
It takes more than a good set of tires to finish a job. It also requires preventative vehicle care and responsive tire repairs. With one of the widest networks of commercial tire stores and service trucks, GCR is there when you need help! Call GCR Tires & Service store near Scandia for 24/7 emergency roadside service, commercial tire mounting, rotation, trailer repair, air pressure checks, alignment, wheel refurbishing, flat repair, and more. You'll get speedy service at affordable prices. You need a partner that can deliver the same level of service no matter where you are in the United States. That's GCR Tires & Service near Scandia.

domain: automotive


text:
Just as in stage three you explored the refusal of the call, in this stage you get to deal with the refusal of the return. Many heroes stop (or pause for an indefinite amount of time) at this stage because they figure they’ve been through enough. They really don’t have to remember their dreams and bring them full circle.
Warm and cozy in their toasty bed in the early morning hours, as the sun is gently filtering its warmth into their sleep zone, the hero has a critical choice to make. Will he or she leave their dreams on the pillow—floating aimlessly and stillborn (harsh, I know, but true) in the dreamtime? Or will the hero summon the courage to retrieve these wisps of dream brilliance and carry them back to the ordinary world?
If you set yourself up properly in stage one by de-cluttering your dream zone and placing your dream journal and a pen on your bedside table (or your phone with your www.DreamsCloud.com app) then you are in good shape to gently make your return. Now is the time to s-l-o-w-l-y roll over and record your remembered dream. This represents your willingness to tip toe your way back toward the ordinary world by remaining in the Hypnagogic State—the zone between sleep and awake—while you record your dream in your journal.
Contemplate the following questions:
•What is your biggest challenge to dream catching in the early morning hours (i.e. being too tired; being afraid to wake up your partner; being alarmed by your alarm clock; your dog or cat jumping on your bed; leaping out of bed too quickly to start your day?)
•Will you take the time to remember your dream(s) tonight?
•How will you catch your dream (i.e. journaling, drawing a picture, using the voice function on your DreamsCloud app, talking with a dream buddy/bed buddy)?
In this episode of weekly radio show, The D-Spot (Mondays at 11am PST on www.AwakeningZone.com), I discuss Stage 10 of the Hero’s Dream Journey (The Journey Back) in more depth: http://www.kellysullivanwalden.com/kelly-sullivan17.html
Stay tuned for next week where we explore The Hero’s Dream Journey Stage Eleven: “Resurrection”…
Kelly Sullivan Walden (a.k.a. Doctor Dream) is a certified clinical hypnotherapist, founder of Dream-Life Coach Training, and author of seven books, most notably the Amazon.com #1 bestselling, I Had the Strangest Dream and Discover Your Inner Goddess Queen. A sought after inspirational speaker, Kelly has spoken, by invitation, at the United Nations. Kelly hosts “The D-Spot” weekly radio show where she explores Dreams, Desires, and Destiny. Kelly has appeared on over 1,000 media outlets including the Ricki Lake Show, and you can see her regularly as FOX news’ Dream Expert. As the past President of Women’s National Book Association (LA Chapter), Kelly has served as a United Nations
NGO representative, which inspired her to create The Dream Project— an educational program that empowers young people to dream to solutions to world issues. Receive free dream gifts from Kelly by going to: www.KellySullivanWalden.com.
This blog has been excerpted from It’s All In Your Dreams…5 Portals to an Awakened Life (Red Wheel-Weiser, Spring 2013).

domain: psychology


Modelling Sediment Behaviour in Constructed Stormwater Wetlands
(2004 – 2008)
Constructed stormwater wetlands are widely used to improve stormwater quality. Modelling sedimentation of suspended solids in wetlands is essential, since a significant proportion of urban stormwater pollutants are transported in particulate form. Many models have been developed for sediment removal, but reliability, robustness and complexity limit applications.
This study developed a new approach for continuous prediction of trapping efficiency of sediment particles through laboratory, field and modelling studies. More than 80 steady-state experiments were undertaken in four mesocosm wetlands and one non-vegetated pond at the Monash Hydraulics Laboratory, exploring sedimentation factors pertaining to wet and dry weather. Based on analyses, a non-linear regression model was developed for prediction of sediment trapping efficiency in constructed stormwater wetlands. The proposed model is physically based, with key coefficients independent of flow rate and sediment characteristics. Field data from a wetland was used to test laboratory findings. The model was modified with background concentration to account for wash-off and re-suspension of fine particles caused by environmental factors. Together with a common flow-hydrodynamic model (CSTRs model), it handles variable flow conditions in real wetlands. Modelling results were compared with those generated from the widely-used, first-order kinetic decay (k-C*) model and proved better suited to varied flow conditions in constructed stormwater wetlands without complex calibration. Further field studies are required for model verification and future refinements.
Click here to read the abstract of this PhD – PDF (50KB)
Li Y. (2008) Modelling Sediment Behaviour in Constructed Stormwater Wetlands. Department of Civil Engineering, Monash University.
Other Publications (link)


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The scale bar represents substitutions per site. NJ trees were constructed using mega 4.1 with 1000 bootstrap replications and Kimura 2-parameter as
a model selleck screening library of nucleotide substitution. All the termites from 14 populations representing two genera, and two families (seven Odontotermes horni, five Odontotermes spp. and two C. heimi colonies), screened initially for the presence of Wolbachia, using PCR assays with the wsp gene were found to be positive. At least one MLST gene was sequenced for all the 14 colonies (Table 1). Repeated failures to PCR amplify Wolbachia genes (MLST and 16S rRNA gene) resulted in incomplete profiles. Sequence typing was performed using the Wolbachia MLST database (http://pubmlst.org/Wolbachia/), which resulted in the assignment of full sequence type (ST) to four strains (Table 1). All of the alleles and allelic profiles characterized for the termite strains were new to the MLST database, except for B supergroup C. heimi Wolbachia (Table 1). Alleles were shared among some of the strains, showing their relatedness, but at the same time showing a distinct difference for other strains. The divergence among the strains accounted for 591 variable learn more sites (VI) out of 2079 sites (28.43%), with a concatenated alignment of all five Wolbachia MLST genes (Table 2). The gene hcpA showed the highest
nucleotide divergence with 154 variable sites out of 444 (34.38%), followed by fbpA with 128 variable sites out of 429 (29.83%) (Table 2). The average Ka/Ks per gene was found to be ≪1 (the average Ka/Ks across genes is 0.0933), which indicated strain evolution mainly by synonymous substitutions. This is compatible with a scenario of a strong purifying selection. Recombination
events were not indicated in either single gene or concatenated MLST dataset alignments (P<0.05) by MaxChi analyses. Phylogenetic reconstructions for all genes by both Bayesian inference and neighbor-joining methods showed similar results, and therefore only Bayesian phylogenetic trees were included. Phylogenetic reconstructions based on the concatenated alignment Bay 11-7085 of hcpA, gatB, coxA, ftsZ and fbpA genes indicated a strong clustering of termite Wolbachia from this study with Wolbachia from bed bug Cimex lectularis and form a sister clade within F supergroup with different species of scorpion genus Opistophthalmus (Fig. 1). Two major clusters were observed for termite Wolbachia in the present study (MCT, W5, G29 and RA) (T1, T3, T21 and THYD) (Fig. 1). On the basis of single-gene analyses, all the strains consistently belonged to the same supergroup. All Odontotermes spp. harbored F supergroup Wolbachia, with the exception of a population from O. horni (T2). Two C. heimi colonies harbored two different supergroup Wolbachia. One of these (TERMITE3) belonged to supergroup F, whereas the other (TLR) belonged to supergroup B (Table 1, Fig. 2).

domain: literature


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A discussion forum for owners and fans of the iconic GRiSO motorcycle from Moto Guzzi. Manufactured in Mandello del Lario on the shore of Lake Como, Lombardy, Italia. The GRiSO was introduced in 2005
A modern day Vampire role play set in Italy. Come and join our coven and our safe house. Here you are free to use your powers without fear of being hunted! Aka Cacciatori di Sangue. Connected though d
The new forum for all you lovers of Italy
Great Fighters Clan Home
The first Italian Satsuki's Worldwide Fan Community Online
Hi everyone !I'm romanian, but I live in Italy, on this forum is you can post all about Android/iOS/Computer tricks/hacks and other.I hope you enjoy this forum!
GIADA VALENTI OFFICIAL FORUM
LEGAL PROTECTION IN ITALY IN CIVIL, COMMERCIAL, CRIMINAL AND IMMIGRATION LAW
516 Ganton Crips forum, so we can discuss shit here, make announcments and so on. [Italy Mafia Roleplay, server. sa-mp. im]
Free forum : PAOK Italia - PAOK Italy forum community
Private forum for The Circle. This forum is related to a Roleplaying game and the content is fictional.Italy Mafia Roleplay - sa-mp.im
Was Aeneas a true hero, or simply a ruthless warrior who happened to stumble upon Italy? This forum is for debate about whether or not Aeneas was a true hero.

domain: hobby


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Nobody wants to have those uninvited guests on your face or suffer from any type of skin-issues before their Big day. If you are heading for your big day and you are suffering from skin issues then this is the treatment for you.
A type of chemical peel is one of the most popular solutions for anyone who suffers from common skin conditions. These conditions, such as acne, wrinkles, age spots or uneven coloration and tone, can all be treated.
It has the ability to resolve a wide array of different skin problems. All you’ll need is a series of sessions that can be performed over a very short duration of time.
Glycolic acid peel is commonly used to treat the following skin conditions –
Wrinkles & Fine lines
Uneven skin tone
Shrinking large pores
Here are the Glycolic acid peel benefits
🔷It is termed as a ‘lunchtime peel’ because of the short duration it requires to complete the treatment.
🔷It rejuvenates the skin and helps in the skin regeneration process.
🔷The dull and damaged top layer of the skin is exfoliated taking away the tan with it.
🔷It brings forward new skin to the surface giving your face a fresh and radiant appearance.
🔷It lightens sun spots, freckles, age spots, hyperpigmented areas, and acne marks.
🔷Another benefit of glycolic acid is that it enhances collagen production in the skin and makes the skin more supple.
🔷It helps to replenish the lost moisture in the skin.
🔷The appearance of fine lines and wrinkles I, the two common signs of aging, is also reduced as collagen production improves and the elasticity of the skin is restored with the absorbed moisture.
🔷Dry and flaky skin is also exfoliated with this peel leaving the skin smooth.
🔷As the exfoliation process removes dead skin and dirt, the skin pores that were blocked with these impurities get unclogged causing a reduction in the pore size. Large pores that appear prominently on the skin become less noticeable.
🔷Unclogged pores also mean that the chances of acne breakouts are reduced as accumulated dead skin cells are removed during the peel


All Along The Watchtower Tab by Jimi Hendrix with free online tab player. One accurate version. Recommended by The Wall Street Journal. 5 Nov 2020 Mark Hamilton's releases 'All Along The Watchtower' (Jimi Hendrix/Bob Dylan Cover) to raise awareness of mental health. Posted on November 29 Nov 2020 According to engineer Andy Johns, Jimi Hendrix had been given a tape of Dylan's recording by publicist Michael Goldstein, who worked for All Along The Watchtower - Jimi Hendrix.
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Find All Along The Watchtower Professional MIDI File & Lyrics. Download MIDI The Jimi Hendrix Experience was a short-lived, yet highly influential rock band famous for the guitar work of Jimi Hendrix (1942– 1970) on songs such as Purple Haze, Foxy Lady, Hey Joe, Voodoo Child (Slight Return) and All Along the Watchtower. Hendrix’s introduction was modest enough to be comic. “Bob, uh, I’m a singer, you know, called, uh, Jimi Hendrix and…” Dylan said he knew who Jimi was and loved his covers of “All Along the Watchtower” and “Like a Rolling Stone.” “I don’t know if anyone has done my songs better,” Dylan said. Lyrics to 'All Along The Watchtower' by Jimi Hendrix: 'There must be some kind of way out of here,' Said the joker to the thief 'There's too much confusion I can't get no relief. 2016-06-17 · Jimi Hendrix “All Along The Watchtower” Production Analysis.
All Along The WatchtowerOst Wolfenstein: The New Order
report. 98% Upvoted. This thread is archived.
jimi hendrix Bokbörsen
Bob Dylan, who had recorded 5 Oct 2012 "All Along The Watchtower" by Jimi Hendrix Listen to Jimi Hendrix: https:// jimihendrix.lnk.to/listenYDSubscribe to the official Jimi Hendrix 2 May 2018 Jimi Hendrix - All Along the Watchtower (Bob Dylan Cover / Live 1970) "All Along the Watchtower" is a song by The Jimi Hendrix Experience, featured on the album Electric Ladyland in 1968 and released as a single in September of 8 Mar 2021 Billboard named Jimi Hendrix's 'All Along the Watchtower' the top hit of '68 based on later digital sales and licensing In its 2014 “Revisionist This 'All Along The Watchtower' classic rock guitar backing track is ideal for getting the timing right during that most famous Jimi Hendrix guitar solo.… As Jimi Hendrix once said, “If there is something to be changed in this world, then it can only happen through music.” -Mark Johnson. Lyrics and Chords. All Along The Watchtower Tab by Jimi Hendrix with free online tab player.
Correction: Jimi Hendrix - All Along The Watchtower (chords) Comment. Preview Add correction. To suggest a correction to the
2 dagar sedan · Jimi Hendrix - All Along The Watchtower (tradução) (Letra e música para ouvir) - There must be some kind of way out of here / Said the joker to the thief / There's too much confusion / I can't get no relief
21 Jan 2016 "Jimi just played a six-string acoustic guitar while Traffic's Dave Mason played 12 -string.
Hur länge gäller säkra lyft
Download MIDI. Show All Jimi Hendrix Free MIDI. Free MIDI Files on MIDIdb.com are demo's with all instruments included. Length of the demo's can vary.
This video contains historical footage taking during the Vietnam War for educational and historical purposes. BACKGROUND MUSIC: The Jimi Hendrix Experience.
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Jimmy Eriksen on Twitter: "Jimi Hendrix Experience All Along
Bob Dylan All Along the Watchtower är en sång skriven av Bob Dylan. Den mest kända versionen spelades in av The Jimi Hendrix Experience redan samma år som 28 juli 2019 — Översättning av 'All Along the Watchtower' av Bob Dylan från engelska till svenska (Version #2) Köp online JIMI HENDRIX 'All Along The Watchtower' German 7" (424870264) • Pop, Singlar, vinylskivor • Skick: Begagnad ✓ Pris 399 kr ✓ • Tradera.com. 9 feb. 2011 — Dagens låt #157 All Along The Watchtower - The Jimi Hendrix Experience Skriven av Bob Dylan Jimi Hendrix - Gitarr & sång Noel Redding Hitta nyckeln och tempot för All Along the Watchtower Av Jimi Hendrix.
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Jimi Hendrix Experience All Along The Watchtower https://youtu.be/qmGFFJtkDXA via @YouTube (Robin Hood studio, UK, 1995) All Along The Watchtower -- Jimi Hendrix.(Isle Of Wight, 8/30/1970) All Along The Watchtower -- Bob Dylan with Mark Knopfler. Här kan du lyssna och ladda ner gratis ringsignal All Along The Watchtower av Jimi Hendrix i formatet .mp3 eller .m4r (för iPhone).
Nyckel, tempo av All Along the Watchtower Av Jimi Hendrix
“JIMI HENDRIX” and related logos are trademarks of Experience Hendrix, L.L.C. "All Along The Watchtower" by Jimi Hendrix Listen to Jimi Hendrix: https://jimihendrix.lnk.to/listenYD Subscribe to the official Jimi Hendrix YouTube Channe 2001-05-08 View credits, reviews, tracks and shop for the 1997 CD release of "All Along The Watchtower" on Discogs. All Along The Watchtower Jimi Hendrix Buy this song. FAVORITE (3 fans) Jimi Hendrix. James Marshall Hendrix (born Johnny Allen Hendrix; November 27, 1942 – September 18, 1970) was an American musician, singer and songwriter.
Format: MP3 Music Verified Purchase. Unlike a lot of people who were around in the late '60s-early '70s, I'm not a particular fan of Jimi Hendrix' drug-induced music. This is my favorite Hendrix song. The Jimi Hendrix Experience began to record their version of Dylan's "All Along the Watchtower" on January 21, 1968, at Olympic Studios in London.