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BLVD is a one-of-a-kind place because its unique amenities set it apart from every other luxury condominium on the Sun Coast. While only 49 fortunate families will enjoy this enviable lifestyle, others will marvel at the innovation and imagination shown in planning the amenities at BLVD.
Most residential developments provide their residents with a few amenities, but the city of Sarasota has never-before-seen an amenity program like this. BLVD features two unprecedented amenity areas on the 5th floor and on the 18th story rooftop.
On the 5th floor, the 11,250sf area includes both indoor and outdoor common areas with a social room, featuring a pool table, game areas, bar, full service kitchen and an expansive, state-of-the-art fitness facility. Unique to BLVD, the area also includes a pet lawn and full-service grooming facility, wine cellar with two temperature controlled wine vaults with private lockable showcases, and an indoor driving range to refine your game anytime you want.
On the rooftop, a 1,200 sf zero horizon pool highlights the 10,000 sf amenity space fully equipped with a covered outdoor summer kitchen with a pair of gas cooktop stations, a gas fire pit, private cabanas and landscape for outdoor yoga and fitness. BLVD also offers an onsite, high end dining and entertainment venue which encapsulates the sought after nightlife of Sarasota without ever having to leave the building.
With an incredible location among all the vibrancy of the Boulevard of the Arts, BLVD is surrounded by the best that Sarasota has to offer, including the world-class Van Wezel Performing Arts Hall, Art Center Sarasota, Asolo Repertory Theatre, Sarasota Ballet, Sarasota Film Festival, Players Theatre, Sarasota Orchestra, Ringling Museum of Art and many more diverse arts organizations. With all these sophisticated cultural venues, you’re living in a community of art lovers, just minutes from Sarasota’s famed white sand beaches and the gentle Bay waters.
The elegant and luxurious residences at BLVD have been thoughtfully designed for today’s contemporary lifestyle. Residents of BLVD will appreciate the innovative design that resulted in some of the most imaginative residences in Sarasota.
The newest and most impressive downtown address is home to just 49 expansive modern condominiums and an unprecedented level of resort-style amenities. The expansive floorplans range from 3550 to over 5900 sf of living space. Each luxury condo offers exceptional views to the east and west including views of the city, Sarasota Bay and the Gulf of Mexico. Residents will enjoy indoor-outdoor living from extraordinary large terraces that range from 1,000 to nearly 5,000 sf of additional space.
Step out of your private access controlled elevator into an expansive Great Room with breathtaking views in every direction. Meander onto spacious terraces and be inspired by a town energized by a collective passion for the arts, entertainment and the incredible natural beauty of its surroundings.
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The prestigious Key Solutions firm will act as the main contact for those interested in making the groundbreaking BLVD Sarasota their new home. Always on the cutting edge of innovation, Key Solutions Real Estate has partnered with IMERZA to incorporate a one-of-kind virtual reality experience showcasing these stunning residences, unlike anything Sarasota has seen before.
With IMERZA’s cutting edge virtual reality technology, buyers can tour, design and decide to buy with confidence. Real-time virtual walkthroughs featuring unparalleled graphics, perspectives and the ability to make real-time upgrades specific to a client’s style allow buyers to see the space as their own. Fixtures, finishes, furnishings and more can be changed at the touch of a button. Homebuyers are able to compose an immersive experience of their new sanctuary and snap a photo for effortless sharing.
BLVD will be the area’s only residential development released in the city this year offering this level of luxury accompanied with single family home sized floorplans.
This is your moment to enjoy all the luxury and convenience of a walkable urban lifestyle in the heart of downtown Sarasota, named the country’s Happiest and Healthiest city by USA Today. Rising above the city and the Sarasota Bay at corner of The Boulevard of the Arts and Tamiami Trail, BLVD Sarasota is destined to become an iconic new architectural landmark.
Our is now open at 500 Tamiami Trail. Don’t miss your opportunity to secure your first selection and special pre-construction pricing during our introductory offering. To join our VIP Interest list, receive our digital brochure or to schedule an appointment, please register today.

domain: design


The new revamp of The Woolpack pub in Emmerdale was unveiled to viewers on Thursday in a special hour-long episode as new owners Charity Dingle and Ryan Stocks had a disastrous opening.
The iconic boozer, which was destroyed in a dramatic explosion at the end of 2021, was previously owned by Chas Dingle and Marlon Dingle.
However, not all viewers were too taken with the new look, with some saying it looked "like a care home".
During Thursday's Emmerdale, Charity and Ryan attempted to have an extravagant opening to unveil the new makeover to the village.
However, things went disastrously wrong due to a low turnout, missing food and an overly loud Bernice Blackstock, which was all documented by a local newspaper reporter.
Yet soap fans were left distracted during the scenes as they were drawn to the new decor in the pub.
And they weren't all best impressed with the new style, with one viewer saying: "Just saw a pic of the new Woolpack interior. That does not look like a village pub. That belongs in Hollyoaks."
Another asked: "What have they done to the #Woolpack ? Yikes ..."
One viewer said the pub was giving the impression of an "old people's home".
One viewer wrote: "Blimey, did the Changing Rooms team decorate the Woolpack? Looks bloody awful!"
Another unimpressed Emmerdale fan said: "Don't like the new look Woolpack, hideous furniture!"
The feedback wasn't all bad though, with some viewers saying they were impressed with the "modern" look.
A different account put: "Loving the new look Woolpack #Emmerdale."
A different viewer put: "@emmerdale I'm loving the new look Woolpack interior #emmerdale."
How to get free TV streaming
Amazon Prime has thousands of original series, sport documentaries and films you can subscribe to watch here for just £7.99/ month plus a free month trial included.
Disney+ is here in the UK and if paid for an annual subscription can save viewers 15%, giving you access to Disney and Pixar films, and popular series such as The Mandalorian. New O2 customers, or existing customers who are upgrading their plan, can get up to 6 free months of Disney+.
Also, Apple TV offer a free trial for seven days but just remember that the subscription will renew automatically at £4.99/a month, so don’t forget to cancel if you don’t want it anymore.
Netflix is available on your smartphone, tablet, smart TV, laptop or streaming device, all for one fixed monthly fee. Plans range from £5.99 to £13.99 a month. No extra costs, no contracts.
It comes after Charity star Emma Atkins said of the pub’s new look: “They have sort of gone for a full refurbishment. They have kept the shape and dynamic and style the same, but I have to say it looks incredible, it’s beautiful.”
The actress added: “[It has] velvet upholstery with a vibrant design and it's so cosy. Quite autumnal in colour scheme, I think it will be fantastic.”
Don't miss the latest Emmerdale news - keep up to date by signing up to our dedicated newsletter here.


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Flow cytometric characterization of acute leukemia reveals a distinctive “blast gate” of murine T-lymphoblastic leukemia/lymphoma
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Zengkai Pan1,*, Min Yang1,*, Kezhi Huang1,2,*, Guntram Büsche3, Silke Glage4, Arnold Ganser1 and Zhixiong Li1
1Department of Hematology, Hemostasis, Oncology, and Stem Cell Transplantation, Hannover Medical School, Hannover, Germany
2Department of Hematology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China
3Institute of Pathology, Hannover Medical School, Hannover, Germany
4Institute of Laboratory Animal Science, Hannover Medical School, Hannover, Germany
*These authors have contributed equally to this work
Zhixiong Li, email: [email protected]
Keywords: flow cytometry; T-ALL; blast gate; midostaurin; FLT3-ITD
Received: September 05, 2017 Accepted: December 05, 2017 Published: December 18, 2017
Immunophenotypic analysis using multiparameter flow cytometry is an indispensable tool for diagnosis and management of acute leukemia. Mouse models have been widely used for medical research for more than 100 years and are indispensable for leukemia research. However, immunophenotypic analysis of murine leukemia was not always performed in published studies, and blast gating for isolation of blasts was shown only in very few studies. No systemic characterization of all types of murine acute leukemia in large cohorts by flow cytometry has been reported. In this study, we used flow cytometry to comprehensively characterize murine acute leukemia in a large cohort of mice. We found that murine T-lymphoblastic leukemia/lymphoma (T-ALL) exhibits a distinctive “blast gate” (CD45bright) with CD45/side scatter gating that differs from the “blast gate” (CD45dim) of human T-ALL. By contrast, murine B-lymphoblastic leukemia and acute myeloid leukemia show the same blast region (CD45dim) as human leukemia. Using blast cell gating, we for first time detected T-ALL development in FLT3-ITD knock-in mice (incidence: 23%). These leukemic cells were selectively killed by the FLT3 inhibitors crenolanib and midostaurin in vitro. These data suggest that FLT3-ITD plays a potential role in the pathogenesis of T-ALL and that FLT3-ITD inhibition is a therapeutic option in the management of patients with T-ALL. Our gating strategy for immunophenotypic analysis can be used for leukemogenesis and preclinical gene therapy studies in mice and may improve the quality of such analyses.
The immunophenotypic analysis of acute leukemia by multiparameter flow cytometry is a powerful tool for proper identification of lymphoid or myeloid lineage and is indispensable in modern diagnosis and management of acute leukemia . CD45/side scatter (SS) gating for isolating blasts by flow cytometry was first proposed by Borowitz and Stelzer [2, 3] and is now widely used. Blasts in the classic “blast gate” (cBG) show CD45dim in CD45/SS gating. However, other cells contaminate the cBG , and blasts may also occur in other gates. Mouse models have been widely used for medical research for more than 100 years and are indispensable for leukemic research. We and others [5–9] have suggested similar proposals for diagnosis of murine acute leukemia, including cytologic, histologic, and immunophenotypic analyses. In mouse models expressing transgene and/or marker genes such as enhanced green fluorescent protein (EGFP) in hematopoietic cells, leukemic cells may be easily isolated by flow cytometry [5, 10–13]. However, in many situations, for example in transgenic mice in which no marker gene was introduced into hematopoietic cells, blast cells may be difficult to distinguish from other cell types. So far, immunophenotypic analysis of murine leukemia was not always performed in published studies, and blast gating for isolation of blasts was shown only in very few studies. No systemic characterization of all types of murine acute leukemia in large cohorts by flow cytometry has been reported. In the present study, we analyze murine acute leukemia by flow cytometry in a large cohort of mice. We show that murine T-lymphoblastic leukemia/lymphoma (T-ALL) demonstrates a distinctive “blast gate” by CD45/SS gating. Our gating strategy for immunophenotypic analysis can be used for leukemogenesis and preclinical gene therapy studies in mouse models and may improve the quality of such analyses.
Murine T-ALL demonstrates a distinctive “blast gate” by CD45/SS gating
We performed experiments for leukemogenesis and preclinical gene therapy studies in more than 3,000 mice, including transplantation of retrovirally modified hematopoietic cells into C57Bl/6J and C3H/HeJ mice, serial transplantation of murine leukemic cells, and studies of transgenic and knock-in mice [5, 10–17]. At least 500 animals developed acute leukemia. A diagnosis of leukemia was established generally based on cytologic, histologic, and/or immunophenotypic findings. Consistent with clustering of human bone marrow cells in CD45/SS gating (Figure 1A), bone marrow cells from healthy mice and mice without hematological malignancies showed similar distribution of cell populations (Figure 1B), except for the location of some B lymphocytes in the CD45dim cBG in most analyzed cases (n = 22) (Figures 1C and Supplementary Figure 1). Contamination of non-blast cells in the cBG has been reported in patients without acute myeloid leukemia (AML) . Blasts from mice with AML (non-monocytic) (n = 31) and B-lymphoblastic leukemia (n = 8) were in the same location in the cBG as blasts from patients with acute leukemia (Figures 1D-1L). Cytological assessment confirmed the presence of acute leukemia in these mice and patients (Figures 1M-1P). While the cBG for human AML and B-lymphoblastic leukemia was clearly separated from granulocytes and monocytes (Figures 1F and 1K), the cBG for murine AML and B-lymphoblastic leukemia was sometimes difficult to set because of the close proximity of granulocytes and monocytes (Figure 1B). Interestingly, blasts from murine T-ALL were located in a gate with the highest CD45 fluorescence intensity (CD45bright) (Figures 2A-2P), whereas blasts from patients with T-ALL were exclusively present in the cBG (Figures 2C and 2D). This distinctive “blast gate” (CD45bright) (Figure 2A) was reproducibly seen for T-ALL in all analyzed mice (n = 74) from different experiments addressing the role of several genes in the leukemogenesis, for example, ΔTRKA , TRKA/NGF , TRKB/BDNF , and p53 (in p53 knock-out and p53/FLT3 double transgenic mice). This blast gate was consistent for T-ALL cells from thymus, spleen, liver, and bone marrow, and for T-ALL with different phenotyping (e.g. CD4/CD8 double positive, CD4 or CD8 single positive, and CD4/CD8 double negative) (data not shown). We proposed this distinctive “blast gate” of T-ALL as an alternative “blast gate” (aBG). Interestingly, this blast region was also seen in Pten-null T-ALL . Moreover, normal T precursor cells isolated from thymus of wild type (WT) mice (n = 2) and one mouse without hematological malignancies were also located in this aBG as T-ALL (Supplementary Figure 1). Importantly, infiltration of T-ALL and AML cells was observed in the bone marrow of some mice, demonstrating different locations of myeloblasts and lymphoblasts in the same sample (Figures 2E-2H). Moreover, we mixed T-ALL cells with AML cells and other cell populations from different mice, and flow cytometric analysis confirmed the same clustering of T-ALL cells (Supplementary Figure 2 and data not shown). Of note, none of the human T-ALL blasts were located in the aBG in a cohort of patients with acute leukemia (n > 200) including ALL (n = 21), but we observed monoblasts in the aBG from some patients with AML M4/5 (Figures 2I-2L) or in the monocyte gate (Figures 1F and 1H) as reported by Gorczyca . Acute monocytic leukemia is rare in mice. Blasts from the mouse with acute monocytic leukemia in our cohort  were also found in the aBG (Supplementary Figure 4).
Figure 1: Clustering of normal bone marrow, AML, and B-lymphoblastic leukemia samples by CD45/SS gating. (A, B) Characterization of normal human and murine bone marrow samples by CD45 gating, respectively. Blue population: T and B lymphocytes (L); green population: monocytes (Mo). Note the slightly different location of murine granulocytes (G) compared with human granulocytes because of less granularity in the cytoplasm of murine granulocytes (B). (B, C) In murine bone marrow, B lymphocytes (purple population) were also present in the classic “blast gate” (cBG, CD45dim in CD45/SS gating). These cells had lower CD19 fluorescence intensity than B cells in the classic lymphocyte gate (blue population). (D) Blasts (red population) from murine AML (mouse #1356) with expression of c-Kit and CD11b (E) in the same location in the cBG as blasts with CD34 and c-Kit expression from patient TI with AML (red population) (F, G). Monocytes from patient TI were heterogeneous and included mature and immature monocytes (CD34pos) (H). (I-L) Blasts from both murine and human B-lymphoblastic leukemia located in the cBG (red populations) (I, J: mouse #1328, K, L: patient NS). (J) Murine B-lymphoblastic leukemia expressing CD19, not CD3. (L) Expression of CD19 and CD10 on the blasts from patient NS with common-ALL. (M-P) Cytology confirmed the presence of blasts in mice and patients (D-I). (N) Note blasts, immature and mature monocytes in patient TI (F-H). M = mouse #1356 (D, E); O = mouse #1328 (I, J); P = patient NS (K, L). M and O: bone marrow cytospins; N: blood smear; P: bone marrow smear.
Figure 2: Different locations of blasts from murine and human T-ALL. (A) Murine lymphoblasts from T-ALL (red population) located in a distinctive “blast gate” (aBG, CD45bright). T = T-lymphoblasts. (B) Murine lymphoblasts expressing CD4, CD8, and CD3 (data not shown). A, B: mouse #1364. (C) Human T-ALL blasts (red population) were present in the cBG (CD45dim). Blue population: lymphocytes; green population: monocytes. (D) Expression of cyCD3 on human blasts. C, D: patient LB. (E) Myeloblasts (red population) and T-ALL blasts (purple population) were located in different regions in the bone marrow of mouse #1329 (E-H). (F) T-ALL blasts expressing CD4/CD8. (G) Expression of CD11b and Gr1 on myeloblasts. (H) Mature lymphocytes expressing either CD3 or CD19. (I-L) Location of monoblasts in the aBG (purple population) from patient TK with AML M4. Note the slightly higher CD45 fluorescence intensity of monoblasts than of the mature lymphocytes (I). Monoblasts were negative for CD117 or CD34 (J), whereas myeloblasts (red population) expressed both CD117 and CD34 (K). (L) Mature lymphocytes expressed CD19 (not CD10) or CD3 (data not shown). (M, N) Bone marrow cytospin and blood smear showing predominant murine and human lymphoblasts in mouse #1364 (A, B) and patient LB (C, D), respectively. (O) Bone marrow cytospin showing infiltration of myeloblasts and lymphoblasts in the bone marrow of mouse #1329 (E-H). (P) Blood smear showing blasts and promonocytes in patient TK with AML M4 (I-L). Blasts were positive for Sudan Black B and non-specific esterase (Supplementary Figure 3).
T-ALL development in FLT3-ITD knock-in mice
Using this gating strategy, we performed immunophenotypic analysis in selected FLT3-ITD knock-in mice, in which an 18-bp ITD mutation was inserted into the juxtamembrane domain of the FLT3 gene . This allows physiological expression of FLT3-ITD from its endogenous promoter and better understanding of the role of FLT3-ITD in leukemogenesis. Homozygous FLT3-ITD/ITD (ITD/ITD) mice develop myeloproliferative disease after a long latency [20, 21]. We found T-ALL in 7 out of 30 (23%) ITD/ITD mice (Figures 3A-3D), in which infiltration of tumor cells was observed in different organs. Other mice developed a (Chronic myelomonocytic leukemia=CMML)-like disease or AML (n=1) as reported (Figures 3E and 3F) [20, 21]. To the best of our knowledge, our report is the first report of T-ALL in FLT3-ITD knock-in mice. Together with an early report showing ALL development in FLT3-ITD transgenic mice , our data suggest a potential role of FLT3-ITD in the pathogenesis of T-ALL. Accordingly, FLT3 mutations have been reported in patients with T-ALL and B-lymphoblastic leukemia , particularly among children with ALL (incidence: up to 25%) [24, 25]. Moreover, leukemic cells from mice with T-ALL were sensitive to FLT3 inhibition (Figure 3G). Crenolanib and midostaurin almost completely killed the cells at 500 nM and 200 nM concentrations, respectively, similar to the concentration (crenolanib) or well below the concentration (midostaurin) safely achieved in humans [26–28]. Our data indicate that crenolanib and midostaurin might be effective in treating the subset of T-ALL patients with FLT3-ITD.
Figure 3: Development of T-ALL in mouse FLT3#457 (FLT3 ITD/ITD) (A-D). (A) Location of T-lymphoblasts (red population) from bone marrow in the aBG (CD45bright) with expression of CD4 and CD8a (B). (C) Bone marrow cytospin showing infiltration of blasts. (D) Liver section showing strong infiltration of blasts. (E, F) CMML in mouse #1382. (E) Flow cytometric analysis showing increased monocytes in bone marrow (green population). Blue population: T and B lymphocytes. (F) Bone marrow cytospin confirming increased monocytes in bone marrow. (G) Leukemic cells from mouse FLT3#457 were sensitive to treatment with FLT3 inhibitors crenolanib and midostaurin. Results are presented as the average percentage of living cells in the presence of inhibitors (100% value derived from DMSO control). Representative results presented are the mean ± SD (error bars) of three independent experiments. Similar results were also observed with leukemic cells from mouse #1361. Cre = crenolanib; Mid = midostaurin.
Mouse models facilitate our understanding of the molecular mechanisms for leukemia development. However, immunophenotypic analysis of leukemic cells is not well investigated, and blast cell gating for isolation of blasts has been performed so far only in very few studies. Guo et al. [18, 29] described the blast gating for T-ALL in an early study. However, they analyzed only leukemia in Pten-mutant mice, and no murine B-lymphoblastic leukemia was characterized. No report has confirmed their finding. Whether the blast cell gating can be applied to other murine leukemias is not clear. In the present study of a large cohort of mice, we extended the finding of Guo et al., and confirmed the same blast gating for T-ALL in different models. Moreover, we analyzed other types of murine malignancies, including B-lymphoblastic leukemia, monocytic leukemia, and lymphoblastic lymphoma, that were not addressed by Guo et al. We demonstrate a distinctive “blast gate” (aBG, CD45bright) of murine T-ALL derived from different mouse models that is different from the blast gate of human T-ALL, whereas other murine leukemia (AML and B-lymphoblastic leukemia) show the same blast region as human leukemia. Use of cBG (CD45dim) for immunophenotypic analysis in cases of murine T-ALL can lead to a wrong diagnosis, particularly in mice without enlargement of the thymus. With the use of blast gating (i.e. aBG), we found T-ALL development in FLT3-ITD knock-in mice (incidence: 23%) that was not reported before in the same models, probably due to the use of different gating. Without the correct blast gating, we would have also overlooked some cases of T-ALL in our model. Pitfalls in the use of the cBG for blast analysis have been also reported in patients without AML .
The reason for this aBG of murine T-ALL is unknown and needs to be determined. CD45 is shown to regulate phosphorylation of kinases such as SRC and JAK family kinases and might have a tumor suppressor role in T-ALL [30, 31]. However, high CD45 expression on leukemic blasts was associated with a poor prognosis in children with precursor B-cell and T-cell ALL, mainly due to a higher cumulative incidence of relapse . In our cohort, mice with T-ALL seemed to have shorter survival than mice without T-ALL. However, in some instances an enlarged thymus might also have contributed to shorter survival of some mice.
FLT3 plays a critical role in the maintenance of hematopoietic homeostasis. In humans, FLT3 is expressed on hematopoietic stem cells, and FLT3 signaling prevents apoptosis in stem cells and progenitors and induces proliferation and maintenance of stem/progenitor cells . Mutated FLT3 has been identified in approximately 30% of AML patients, making it one of the most common mutations with prognostic implications observed in this disease. Frequently, the mutation is an in-frame internal tandem duplication (ITD) in the juxtamembrane region or a point mutation in tyrosine kinase domain-1 that results in constitutive activation of FLT3. A recently published trial that included 717 patients with previously untreated FLT3+ AML demonstrated a statistically significant improvement in overall survival (OS) for patients treated with midostaurin (targeting FLT3-ITD) compared with those on the placebo-containing arm (HR 0.77, P = 0.016) . This led to recent FDA approval of midostaurin as frontline therapy for adult patients with newly diagnosed AML. In this study, our data suggest a potential role of FLT3-ITD in the development of T-ALL. We showed that murine T-ALL induced by FLT3-ITD is highly sensitive to treatment with FLT3 inhibitors, including midostaurin. Early studies demonstrated that ALL samples from patients with FLT3 mutations or high level of FLT3 expression were selectively killed by FLT3 inhibition . These data suggest that targeting FLT3-ITD might be a treatment option for ALL patients with FLT3 mutations or high FLT3 expression.
In conclusion, our study is the first comprehensive study that characterizes all common types of murine acute leukemia in a large cohort of mice by flow cytometry. Careful immunophenotypic analysis of leukemic cells with the right blast gating may improve the quality of mouse experiments. Because both murine and human blasts can be in both blast gates (cBG with CD45dim and the aBG with CD45bright), our data highlight careful isolation of blasts in acute leukemia by flow cytometry, and the proposed aBG is thus helpful for making appropriate diagnoses of T-ALL in mouse models. Moreover, for clinical diagnosis and management of patients with acute leukemia, it should be aware that leukemic blasts can be also isolated from the aBG with CD45bright.
MATERIALS AND METHODS
In vivo tumorigenesis assays
Retroviral transductions of murine hematopoietic cells and in vivo tumorigenesis assays have been described [5, 10-16, 35]. We purchased FLT3-ITD knock-in (KI) mice from the Jackson Laboratory, in which an 18-bp ITD mutation was inserted into the juxtamembrane domain of the FLT3 gene. The mice with C57BL/6 background were originally established in the Gilliland´s laboratory . Upon arrival at the Jackson Laboratory, the mice were crossed to C57BL/6J mice at least once to establish the colony. After arrival at our animal facility, the mice were crossed to C57BL/6J at least three times in order to obtain a high C57BL/6J background. FLT3-ITD mice were crossed to p53 knock-out mice to generate double transgenic mice. Animal experiments were approved by the local ethical committee and performed according to their guidelines.
At the end point analysis, mice were macroscopically examined for pathological abnormalities during dissection. Enlarged organs were weighed. Bone marrow, spleen, liver, skin, gut, kidney, lung, brain and thymus were fixed in a buffered 4% formalin solution and embedded in Paraplast Plus (Kendall, Mansfield, MA, USA). Sections were routinely stained with hematoxylin and eosin. Blood cell counts were measured by an automatic analyzer (ABC Counter, Scil, Viernheim, Germany). Cytological and histological examinations were performed as previously described .
Flow cytometric analyses
Flow cytometric analyses were performed by use of whole murine bone marrow/spleen cells and patient samples after Ficoll separation (thus lower erythrocytes and debris in patient samples; see e.g. Figure 1A). Murine cells were stained with fluorescein isothiocyanate (FITC)-conjugated, R-phycoerythrin (PE)-conjugated, allophycocyanin (APC)-conjugated, APC-eFluor 780-conjugated, Percp-cy5.5-conjugated, or PE-Cy7-conjugated antibodies, including CD45, CD34, CD117, CD11b, Gr-1, Ter119, CD71, F4/80, CD4, CD8a, CD3, CD19, B220, CD44, and CD25 (from eBioscience or Biolegend). Human cells were stained with fluorescein isothiocyanate (FITC)-conjugated, R-phycoerythrin (PE)-conjugated, allophycocyanin (APC)-conjugated, APC-H7-conjugated, Percp-cy5.5-conjugated, or PE-Cy7-conjugated antibodies, including CD45, CD34, CD117, myeloperoxidase, CD13, CD14, CD15, CD33, CD61, CD235a (glycophorin A), HLA-DR, CD3, CD4, CD8, CD1a, CD2, CD5, CD7, CD8, Tdt, TCR α/β, TCR γ/δ, CD19, CD10, CD20, CD20, CD79a, kappa, lamda, and IgM (from BD Biosciences or Biolegend). Intracellular staining was performed using an IntraPrep Permeabilization Reagent Kit according to the manufacturer’s protocol (Beckman Coulter, Marseille, France). The cells stained with antibodies were analyzed by flow cytometry either on an FACSCalibure or FCASCanto (BD Bioscience). Generally, atleast 10,000 cells per tube were measured and analyzed. Leukemic blasts were analyzed on CD45/SSC plots as previously described [2, 3].
Leukemic cells were cultured in the presence of inhibitors for 48 hours before apoptosis analysis. Cell viability was analyzed using the Annexin-V assay (BD Pharmingen, Heidelberg, Germany) on an FCASCanto. We generally measured and analyzed at least 10,000 cells per tube. Annexin V+/PI- and Annexin V+/PI+ cells are considered cells in the early stage of apoptosis and the late-stage of apoptosis, respectively. The inhibitors crenolanib and midostaurin were purchased from Selleckchem (Houston, TX).
We are very grateful to Jolanta Adolf, Christine Garen, Ellen Hebold, and Kerstin Schantl for technical assistance. We thank Matthias Gaestel and Karl Lenhard Rudolph for providing p53 knock-out mice.
CONFLICTS OF INTEREST
The authors do not declare any competing financial interest.
This work was supported by the Deutsche Forschungsgemeinschaft (DFG grant: Li 1608/2-1), Deutsche José Carreras Leukämie-Stiftung (grant: 13/22), and Alfred & Angelika Gutermuth-Stiftung (projects 16/3 and 17/3). ZP and KH were supported by the China Scholarship Council (201406100008 and 2011638024). GB and SG were supported by the DFG excellence cluster REBIRTH.
ZP and MY performed research and collected, analyzed, and interpreted data. KH and SG performed research. GB performed histological analysis. AG performed cytological analysis and provided administrative support. ZL conceived the concept, performed research, collected, analyzed, and interpreted data, and wrote the manuscript.
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domain: literature


text:
Company Overview of ACE Cash Express, Inc.
ACE Cash Express, Inc. provides installment loans, title loans, payday/renew loans, prepaid debit cards, cash advances, and auto insurance. The company also provides bill payment, money transfer, security, check cashing, money order, direct deposit, tax check, and online services. It serves customers through stores in the United States. The company was founded in 1968 and is headquartered in Irving, Texas.
1231 Greenway Drive
Irving, TX 75038
Founded in 1968
Key Executives for ACE Cash Express, Inc.
Chief Financial Officer and Executive Vice President
Chief Operating Officer and Executive Vice President
Chief Information Officer and Senior Vice President of Information Systems
Chief Administrative Officer
Compensation as of Fiscal Year 2014.
ACE Cash Express, Inc. Key Developments
ACE Cash Express Agrees to $10 Million Settlement with Consumer Financial Protection Bureau
Jul 10 14
The Consumer Financial Protection Bureau (CFPB) announced it had reached a settlement with ACE Cash Express, and that the lender had agreed to pay a $5 million penalty and $5 million in refunds. The CFPB had accused ACE Cash Express of using illegal debt collection practices to pressure borrowers into taking out additional payday loans. ACE used false threats, intimidation, and harassing calls to bully payday borrowers into a cycle of debt. The company said that it has fully cooperated with the CFPB and that it hired Deloitte Financial Advisory Services to review a statistically significant, random sample of collections calls its employees made between March 2011 and February 2012. From that review, the company said 99.5% of customers with a loan in collections for more than 90 days did not take out a new loan with ACE within two days of paying off their existing loan, and 99.1% of customers did not take out a new loan within 14 days of paying off their existing loan. The company settled this matter in order to focus on serving its customers and providing the products and services they count on.
ACE Cash Express, Inc. - Special Call
Jul 10 14
To discuss Settlement with Consumer Financial Protection Bureau
ACE Cash Express, Inc. to Report Q3, 2014 Results on May 14, 2014
May 8 14
ACE Cash Express, Inc. announced that they will report Q3, 2014 results at 8:00 PM, GMT Standard Time on May 14, 2014
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domain: finance


text:
Celebrating 200 years of Medina Sandstone was the topic for the 2023 John Ryan Scholarship. The John Ryan School of Historical Excellence Scholarship is an annual award given to a deserving Medina High School senior who has enrolled in an exclusive program called the John Ryan School. The program was named after John Ryan who was the owner and first person to start a quarry in Medina in 1837 – naming his product Medina Sandstone.
This year’s winner of the $1000 scholarship was Aiden Paul, son of Mike and Julie Paul of Medina, NY. Paul’s essay and Powerpoint presentation that won him the prize was entitled – “What is Medina Sandstone”. Paul will be attending Niagara County Community College in the fall majoring in business. He also will be playing baseball while attending NCCC.
The 2023 John Ryan Scholarship Committee members that helped to choose the winning essay were Kathy Bogan, Matt Holland, Rob Klino, Craig Touhey and Mary Zangerle.
The award ceremony took place on June 20, 2023 in Medina City Hall in the Sandstone Hall of Fame. Todd Bensley and Margaret Martin attended the ceremony representing the Medina High School History Department and Marguerite Sherman, Deputy Mayor was also in attendance representing the Village of Medina. A reception immediately followed the ceremony and was hosted by the Medina Sandstone Society’s Event Committee.
The John Ryan School was started in 2015 and has awarded over $8000 in scholarships. The purpose of the John Ryan School of Historical Excellence is to bolster and instill in the minds of Medina’s youth a heighten pride in our local community.

domain: history


text:
The purpose of the NIST Manufacturing Extension Partnership (MEP) Advisory Board as stated in statute [15 U.S.C. § 278k (m)] is to provide the NIST Director advice of the activities, plans and policies of the Program and assessments of the soundness of the plans and strategies of the Program as well as assessments of current performance of the plans of the Program.
Law requires the MEP Advisory Board consist of not fewer than 10 members appointed by the NIST Director whom broadly represent the interests and needs of the manufacturing sector. The law also requires at least two members be on an advisory board for an MEP Center, at least five members be from U.S. small businesses representing the manufacturing sector, and at least one Board member shall represent a community college. The Board members today represent the diversity of the U.S. manufacturing industry, from CEOs and executives representing various small to medium-sized manufacturers to academic leaders located at various institutions of higher education..
In addition, the Board members are limited to two consecutive three-year terms and are ineligible for reappointment for one year. Finally, law requires the Board to meet at least twice per year.

domain: law


text:
Juaso (Ash), Sept 20, GNA – Police Superintendent Bossman Ohene-Boadi, the Konongo Divisional Commander, has renewed the appeal for increased public support to rein in people, who have chosen to live outside the law.
He said it was important to provide them with useful leads – credible information, for the arrest and prosecution of criminals.
He also called for the supply of vital logistics to enable them to efficiently perform their duties to ensure public safety and security.
He was speaking when receiving two motorbikes bought for the Juaso police by the Asante-Akim South District Assembly.
The motorbikes, which cost GHȼ10,400.00, are meant to help enhance the mobility of the police to aid crime fight in the district.
He spoke of their determination to continue to work with total commitment to protect lives and property – to make the society a safer place for everybody.
He gave the assurance that the motorbikes would be put to good use and properly maintained.
Mr. De-graft Forkuo, the District Chief Executive (DCE), pledged to do everything they could to support the police to perform.
He entreated the officers to uphold high standards of professionalism and remain vigilant, as the nation inched closer to general election.
Deputy Superintendent of Police (DSP), Samuel Sandow Latebu, the District Commander, commended the assembly for the gesture.

domain: topicality


text:
Abdominal Pain Doctors Near You
Need to make a doctor appointment for Abdominal Pain this week? Use Zocdoc to find doctors near you who take your insurance. It’s simple, secure and free.
Dr. Kodroff was very kind and down to earth. The wait time might be the best I've ever experienced. I was able to get a same day appointment as a new patient which was great. The check in process was also quick and efficient. I recommend this office.
Find doctors and make appointments online
Managing your healthcare is easier than ever before with Zocdoc. Just search for a doctor in your insurance network, see available times, and book an appointment on the spot! You can read real doctor reviews from other patients, see the doctor’s background and education, view photos of the office, and more. It’s simple, secure, and free!

domain: medical


text:
Overlooking Lake Minnewaska
A scenic view of Lake Minnewaska in Ylster County New York.
The Carriage House
The historic Carriage House in Allaire village was a stage coach stop dating back to the early 1830's.
Historic Allaire Millstones
Historic old millstones and brick building during Autumn in Allaire State Park in New Jersey.
Scenic Autumn Pond
A wetland pond in Allaire State Park in Monmouth County New Jersey.
Autumn Pond Reflections
Colorful Autumn foilage overlooking this pond in Allaire State Park in New Jersey.
Morning Sunlight In Park
Bright sunshine and an early morning view of Thompson Grove Park in Manalapan New Jersey.
Dead Old Stump
An old dead stump in Thompson Grove Park in Monmouth County New Jersey.
Morning chemtrails over beautiful Thompson Grove Park in Manalapan, New Jersey.
Fog on Millhurst Pond
Early morning fog on Millhurst Pond in Thompson Grove Park in Manalapan, New Jersey.
Early Morning Pond View
Scenic Millhurst Pond on an early Autumn morning in Thompson Grove Park in Manalapan New Jersey.
George Lake Panorama
The swimming beach surrounded by Autumn leaves in Schooly's Mountain Park in Washington Township New Jersey.
George Lake Reflections
Autumn reflections on George Lake in Washington Township New Jersey.
Golden Autumn Sunshine
Vibrant Autumn leaves in Schooly's Mountain Park in Morris County New Jersey.
Rancocas Creek Reflections
The Rancocas Creek in historick Smithville in Burlington County New Jersey.
Waterfall and Dam
A dam and waterfall in historic Smithville New Jersey.
Holiday Lake Sunset
A sunset panoramic view of Holiday Lake in Manalapan New Jersey.
Holiday Lake Panorama
An Autumn panoramic view of Holiday Lake in Manalapan New Jersey.
Colorful Autumn Leaves
The changing Fall colors as seen along Holiday Lake in Manalapan New Jersey.
Dusk on Autumn Lake
A scenic view of Holiday Lake in Manalapan NJ just before sunset.
Colorful Autumn Day
Blue sky and vibrant autumn colors in Upper Freehold New Jersey.
Autumn Day Dock on Lake
An early Autumn scene of Prospertown Lake in Jackson New Jersey.
Horse and Bridle
A close-up of a horse with bridle on a New Jersey horse farm.
Psychedelic Autumn Pond
Colorful abstract Fall colors on this pond in Upper Freehold New Jersey.
Horses Along the Highway
A horse farm along the road in rural Central New Jersey.

domain: travel


text:
Objective: To estimate the CKD (GFR <60 ml/min and/or UACR ≥ 30 mg/g) prevalence among T2DM patients and evaluate the use of antidiabetic drugs based on renal function.
Methods: Retrospective, cross-sectional study, analyzing data from the Spanish electronic records database “Information System for the Development of Research in Primary Care, SIDIAP”, from Jan, 2015 to Dec, 2016. The use of antidiabetic drugs for each stage of chronic renal failure (CRF; GFR<60 mL/min) was evaluated in patients with available records of GFR. Furthermore, the adjusted RR for 5 events (global mortality, cardiovascular mortality, renal failure treated with dialysis or transplantation, acute renal failure and progression of kidney disease) according to KDIGO 2012 guidelines were assessed in those patients with available registers of both UACR and GFR.
Results: From the overall T2DM study population (373,185), 33% (122,296) had CKD, of those, 48% were women, with a mean age of 76 years, a mean T2DM duration of 11 years and a mean HbA1c value of 7.2% (SD 1.37). A total of 336,198 patients (90%) had GFR data, and 94,240 (28%) had CRF. Among those with GFR 30 to < 45 mL/min (29,038; 8.9%), metformin was the most frequently used drug (49.6%), followed by insulin (33.5%), DPP-4i (21%) and sulfonylureas (15.9%). Among patients with GFR < 30 mL/min (13,262, 4%), insulin was the most used drug (50%), followed by DPP-4i (23%), metformin (16%), repaglinide (16%) and sulfonylureas (6.1%). The KDIGO 5 event-adjusted RR distribution among the 236,830 patients with available values of both UACR and GFR was: Low: 60.9%; Mild: 21.6%, High: 9.8% and Very high: 7.7%.
Conclusion: CKD is a very frequent comorbidity among T2DM patients. The use of antidiabetic drugs is more complex in these patients because many people with kidney disease are often elderly, and have long lasting disease and significant comorbidities. A relevant number of T2DM patients are still treated with contraindicated antidiabetic drugs regarding their renal function.
M. Mata-Cases: None. J. Franch-Nadal: None. J. Real: None. K. Ferreira de Campos: Employee; Self; Merck Sharp & Dohme Corp. M. Cedenilla: Employee; Self; Merck Sharp & Dohme Corp. A. Gómez: Employee; Self; Merck Sharp & Dohme Corp. D. Mauricio: Advisory Panel; Self; AstraZeneca. Research Support; Self; AstraZeneca. Speaker's Bureau; Self; Eli Lilly and Company, GlaxoSmithKline plc.. Research Support; Self; GlaxoSmithKline plc.. Advisory Panel; Self; Janssen-Cilag Pty Limited. Speaker's Bureau; Self; Merck Sharp & Dohme Corp.. Board Member; Self; Merck Sharp & Dohme Corp.. Research Support; Self; Merck Sharp & Dohme Corp.. Advisory Panel; Self; Novo Nordisk A/S. Speaker's Bureau; Self; Novo Nordisk A/S. Advisory Panel; Self; Sanofi. Board Member; Self; Sanofi. Speaker's Bureau; Self; Sanofi. Research Support; Self; Sanofi.

domain: statistics


MTV recently nabbed an interview with Mark Wahlberg and asked him about the rumors that he’s involved with the Uncharted movie, which is of course based on the best-selling PS3 series. He didn’t hesitate at all in saying that he’s up for the main character of Nathan Drake. What’s more, David O. Russell (Three Kings, The Fighter) is writing and directing, and there might be roles for Robert DeNiro and Joe Pesci.
Wow. How would those two fit in?
“I’m obviously in whatever David wants to do but the idea of it is so off the charts: De Niro being my father, Pesci being my uncle. It’s not going to be the watered-down version, that’s for sure.”
Could definitely see DeNiro being a good Sully.
Check the interview out below.


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|Index||38 reviews in total|
A version of this comparison has already been posted over at "Salvador"
Salvador is Olvier Stone's best work and James Woods' finest performance. Perhaps my only regret about this movie has to do with it not going nearly far enough in depicting the brutality of the US client regime in El Salvador. But this observation does not count, as it doesn't have anything to do with the film as presented. A critique of Salvador would do much better to note that there are very few films about the political situation in Central America, period. Persons who are interested in the subject matter might do well to compare this Stone effort with the much earlier Under Fire (1983), a film which boasts superlative performances by Nick Nolte and Gene Hackman. Under Fire is perhaps one of the most under appreciated films, not just of the 1980s, but of all time. Both Under Fire and Salvador are head and shoulders above Ken Loach's limited tale of a Nicaraguan refugee's individual trauma - Carla's Song (made much later in 1996). Both earlier films were made at the time Central America was a major obsession of the Reagan Administration (which went so far as to suggest AK-47 toting Sandinistas were about to invade the Texas border). On account of this background alone, the respective cast and crews of both films deserve the sort of praise we should usually reserve for true artists rather than Hollywood's employees.
Both Salvador and the much earlier Under Fire are very close in their subject matter: portraying disinterested journalists who only after becoming aware of the gravity of the situation in which they find themselves turn unsympathetic towards clients of the American Empire. The sort of journalists which have been entirely purged from the corporate-owned "mainstream" or "embedded" press in the United States (and the EU too).
Both films do an outstanding job of noting the protagonists' rivals in the form of spin doctors for the regime whether from the US State Department or the corporate media. Characters like Salvador's ANS reporter Pauline Axelrod (played by Valerie Wildman) force us to recall the perverted scribblings of James Lemoyne (New York Times), the godfather of Embedded American Journalism; his students honored in that tribute to the corporate press, Welcome to Sarajevo (1997). Call that film for what it is: the anti-Salvador.
Under Fire goes much deeper than Stone's film in questioning the ethics of journalism and the sort of circumstances which compel individuals to look at the bigger picture. The depiction of the conflict between Hackman and Nolte, on both personal and professional levels, makes it a very rewarding film. Salvador's portrait of a troubled has-been photojournalist who undergoes a sort of radical shock therapy in a war zone is different, but certainly no less interesting.
I have to give the decisive edge to Under Fire for drawing much more attention to the nature and breadth of the foreign support upon which the corrupt Central American dictatorships relied. Salvador has a US helicopter turn up in the middle of a battle, an ambassador portrayed as indifferent, and that's about it. Under Fire, in contrast, has excellent performances by a young Ed Harris and Jean-Louis Tritignant as pro-regime killers, roles which draw attention to the nature and morality of those embattled dictatorships.
Salvador counters with a much more interesting profile of some of the members of the so-called "government" and its military. In Under Fire, we just see Anastasio Somoza depicted as an insignificant car salesman type in the background who also happens to be the latest heir to the dynasty which ruled over Nicaragua for much of the 20th century. This was a wee bit dissatisfying.
The major differences between the films are technical and stylistic. Some may prefer Stone's use of tight editing and rather fanciful action sequences. I personally preferred Under Fire's determined efforts to bring out as much stark realism as possible on screen especially in the battle scenes, which are among the most authentic attempts to portray urban and guerrilla warfare in the history of cinema. No, it's not as pretty as Tom Cruise dropping bombs to the accompaniment of Kenny Loggins, and any film which reveals as much deserves special praise. One wonders if "Under Fire" or "Salvador" could be made in Hollywood today.
A 9/10 for Salvador and a 9/10 for Under Fire, and again hats off to all associated with films which one can hardly imagine being made in this Orwellian or "embedded" age.
*** This review may contain spoilers ***
Good films about politics are rare. Films which successfully combine
politics and a love story are even rarer. "Under Fire" manages this
Nick Nolte, Gene Hackman and Joanna Cassidy give superlative performances as the trio of journalists caught up in the passion and excitement of the 1979 Sandinista revolution in Nicaragua. That wonderful photographer John Alcott catches some magical images and Jerry Goldsmith composes a score so good you will want to seek out the album.
This is a film full of moments that will live in your memory, not least the shocking, tragic consequence of Nolte's willingness to assist the rebels. It brings home the chaos and confusion of war but also its moments of elation : "I'd do it again", says Nolte at the triumphant conclusion. Before that, the spy Jazy, played by Jean-Louis Trintignant, an urbane, cultured but completely amoral killer, tells us that we will only know the truth of what happened in Nicaragua once 20 years have passed.
Well, those 20 years are now behind us. The right side won. And this film, with its eloquent photography and soaring soundtrack, may turn even the most apolitical person into a Sandinista.
"Under Fire" is a well-written, well-acted piece, showing photo-journalists operating in the milieu of insurrections in Chad, then Nicaragua. Watching Gene Hackman, Nick Nolte and Ed Harris perform together was a treat. And the writers gave them terrific lines. "This is a great war: good guys, bad guys, and lots of cheap shrimp." I especially liked when Hackman's character asked if Nolte's character had slept with Hackman's woman when their relationship hits the skids, and Nolte answers directly, "Hell no, Alex. We're friends." And you just know Nolte's character meant it, man to man. Great moment. Also appealing was the way third-world conflicts were portrayed as global brushfires; put out one here, while another flares up over there. Using the real civil war in Somoza's Nicaragua gives the film unexpected credibility. And probably in keeping with reality, Ed Harris has several memorable scenes as a pure mercenary, a globe-trotting soldier-for-hire, who shows up where the gun-battle action is. His last line is something like "See you in Laos". The beat goes on. -ejpede
This film was a surprisingly quality portrayal of the difficulties
faced by those in underdeveloped countries too often overrun by corrupt
It is presented through the eyes of a photo-journalist (played by Nick Nolte) & his contacts, as they pursue the news stories we in supposedly advanced nations, witness each day on our television screens. Of course, it is subjective but presented with an appropriate sense of the drama & courage that's needed to bring such coverage of gross injustice to the detached conscience of those whose governments often make insensitive contributions to the peoples, mainly peasants & the oppressed. These poor & downtrodden people cannot speak for themselves & rely on such photojournalism to be their mouthpiece to the wider world. It has applications far beyond Nicaragua, across all continents, for human rights' abuse was rife 20 years ago when the film was made, & is today, & likely will be far beyond.
Unlike too many modern movies that are action-filled with special effects but largely without plot, this movie does deliver. The central figure portrayed engages in a series of hit & run encounters with the authorities & its mostly ruthless army of foot soldiers. He & his associates live on their individual & collective wit's end. Within seconds, the victims can go from pursuer to the pursued. Let alone the predicament that local peoples find themselves in, for they would rarely if ever, be accepted into the supposedly developed nations whose propaganda currently rules the world, no matter how unjustly or offensively or insensitively it is applied.
Likewise, the survival of the photojournalists & their associates, are caught in dilemmas of conscience. For the oppressed peoples they dare to cover the struggles & injustice & suffering of, seem to be meat in the sandwich of leaders who use & abuse such locals, as puppets. Journalists often depend on the contacts they form, however transcient their interaction. The woman who beckons him into a backyard sanctuary; the woman who refers a request for directions to the authorities; a priest tortured & suffering unjustly while sharing a jail cell; the occasional compassionate soldier with heart enough for his potential victims vs dictatorial unjust judgements; people willing to bravely die for their cause in the name of their causes of their heart. Such as these present unpredictable twists adding to the unfolding drama, where war is being found & fought on many levels, personal & within or beyond organisations.
As such, "Under Fire" gives the viewer a reality in which to help a viewer to understand much more than it presents, or dares to represent. The roles of friendship, empathy & compassion present in many unlikely forms, so too, the consequences, even fatality, from the slightest failure to read the signs or sense danger, while the ruthless pursue goals without concern but for their hierarchy of self-made regulations & adherence to them.
All up, a quality movie not to be missed, and one which is likely to linger & enrich your appreciation of war correspondents of integrity & conviction, willing to lay their lives on the line.
A sophisticated film with more than one level, while its sympathies obviously tend to lie with the Sandinistas, it also has the message that in war there are no moral absolutes, and raises some interesting dilemmas. Its portrayal of violence is brutally convincing without being gratuitously gory. One possible flaw in Nolte's character is that it's hard to believe that a man apparently given to fairly regular reckless behaviour would have lasted so long hanging around in war zones.
As a Nicaraguan-American who lived there during the opening thunderclaps
the full scale Sandinista revolt, I must say I was extremely impressed
this movie as a whole. Although it takes a slight turn to the political
left, it manages to keep the story on an even keel and not embelish so.
It is interesting that by far the truest insight is delivered by the cynical French opportunist. Tyranny and oppression lay on both sides of the political fence. If the right hand doesn't get you, the left one will. When the FSLN took power in 1979, they immediately announced their communist regime much to the chagrin of the populace (personally, I believe in this crazy little thing called "freedom").
The people who were just liberated from 40 years of right wing (US supported) tyranny, now had it from the (Soviet supported) left, and then some. Proof of this was the mass exodus of Nicas to other places, and the (US backed, of course) "Contra" rebels, made up of former Sandinistas who immediately took up arms against their former comrades, and fought for a proper democracy, which was finally achieved when the USSR folded its cards in the late 80s.
Nicaragua was then free.
This movie really hits the mark in many ways. It's the best movie of its
In the opening scene in some unspecified African civil war Nick Nolte, war
journalist, discovers Ed Harris, mercenary, riding in the wrong truck
surrounded by his enemies. Harris hasn't realized that after the
of the battle he climbed in a truck of soldiers from the opposite side.
They in turn haven't realized that Harris isn't their mercenary. Harris
says, `I guess they'd really be p***ed if they knew.'
This scene sets the theme for the movie perfectly. Not only doesn't the
mercenary care which side he is on, but it is implied that the sides are
pretty much interchangeable and it doesn't much matter who's truck we
This is pretty much Nolte's attitude as he travels from one war to
We begin to suspect he isn't that different from Harris.
But affairs in Nicaragua make his neutrality seem immoral and he is forced
to choose between his journalistic ethics and his humanitarian ones.
Great writing is matched by great acting from Hackman, Harris and Nolte and Johanna Cassidy.
If you want a documentary about the Sandinista's, go watch a documentary. If you want a thrilling love-story set around a fast-paced, intelligent script about people who want to do the best they can under difficult circumstances, then this is the film for you. Good acting, superb music, a good use of locations and atmospheres. This must be one of Nolte's best performances. Of course Rafael did not exist, but this is a movie, not a portrayal of real facts. The story works perfectly in this movie, and that is what's important. If you're looking for a flick that entertains, touches you without being too sentimental, and you like some action, then Under Fire is certainly worth seeing.
I have revisited this film after many years, primarily to see the Gene Hackman that is and has always been and the Nick Nolte that was. The love triangle (Hackman/Cassidy/Nolte) is awkward and unnecessary but the pace of the events depicted are satisfyingly fast-moving and expertly set up. This particularly applies to the rooftop skirmishes early in the Nicaraguan scenes and especially the finale, when Nolte's photographer is hounded around town. The confrontation between Hackman's TV star and Somozas military is one of those "DID YOU SEE THAT" moments which are frightening because they are oh so believable. Hackman, Nolte and Cassidy apart, there are very very watchable performances from Ed Harris as an amoral globe-trotting mercenary and Jean Louis Trintignant as a deeply devious master manipulator. Like the other South American political classic "Salvador" this very good movie has impressively stood the test of time.
This is Nick Nolte at his best in a first rate romantic thriller. Set in Nicaragua but filmed in Mexico, Under Fire captures the look and feel of revolutionary Central America, easily drawing the viewer into the horror of life under the Somosa puppet regime. If you liked "Under Fire" check-out "Salvador" or "Romero" for the same gritty realism -- these are 3-movies that cause one to think and to question.
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About ISAT - Profile
The Irish Sleep Apnoea Trust (ISAT)
a non-political, non-profit making Voluntary Patient Support Group for
sufferers (and their families) of Sleep Apnoea.
The ethos and management style of the Irish Sleep Apnoea Trust is one of transparency in its decision making process coupled with a commitment to serve and deliver benefits to the members. Effective communication is a cornerstone of this style.
To ensure delivery of these and our principal aims, our Committee is elected annually and is governed by membership approved Rules, and Accounting and Administrative Procedures and Controls. This is in addition to the normal regulations governing corporate bodies.
Regular contact is also maintained through our newsletter and email
updates. Our web site is used as an information, awareness and
communication portal for our members and the general public. It also
acts as an important communications portal for interaction with other
groups, feedback and membership surveys and general discussion.
A Management Committee, comprising the Honorary Officers (Chairman, Vice Chairman, Company Secretary, and Honorary Treasurer) and a representative from our Medical Council deal with the day to day running of the Trust.
Establishment of ISAT
ISAT was established in May 2000 following an initial exploratory
meeting of patients with obstructive sleep apnoea syndrome (OSAS), at
St. Vincent's University Hospital, Dublin 4. The initial specific
aims of the Trust were to support patients with OSAS and their families,
to promote improved facilities for the investigation and management of
this disorder, and to support medical research. A steering
committee initially worked over the lead 9 months to develop a detailed
set of rules and regulations in addition to articles of association.
Almost 200 patients with OSAS signed up to attend the inaugural Annual
General Meeting. The First annual meeting of the Irish Sleep
Apnoea Trust (ISAT), in Irish, "Iontaobhas Apnoea Codlata na hÉireann"
was held at St. Vincent's University Hospital, Elm Park, Dublin 4 on
Saturday 3rd March 2001. Delegates were asked to and approved the
rules and regulations and articles of association. A formal committee
structure was established for the Trust. It was envisaged that, as
interest grew, regional branches of ISAT would be established throughout
The Respiratory Sleep Disorders Unit at St. Vincent's University Hospital was established in 1985 and provides a national referral service for the investigation and treatment of patients with OSAS. Treatment with nasal CPAP is highly effective and can transform the lives of affected patients.
The Aims of the Irish Sleep Apnoea Trust
To increase public and professional understanding of the significance of the diagnosis and the correct treatment of Sleep Apnoea in terms of good health, safety and the economy.
To reduce the incidence of health and safety problems related to insufficient sleep and undiagnosed and untreated Sleep Apnoea.
To actively lobby for and support increased education about and scientific research into the diagnosis and treatment of Sleep Apnoea.
To advocate public policy initiatives which promote education about, research into and treatment of Sleep Apnoea.
To research and collate information on the dangers to sufferers of Sleep Apnoea and disseminate this information to the public, patients, healthcare professionals, the appropriate public authorities and employers through active advocacy, information leaflets, media releases, educational events and a website.
To work with government agencies, professional bodies and industry to increase public awareness of the problems related to Sleep Apnoea.
To promote evidence-based best practice and promote the availability of such clinical services for Sleep Apnoea sufferers in all areas of Ireland.
To seek governmental, charitable and commercial funding for research into Sleep Apnoea to allow improved treatment.
Achieving the Aims of the Irish Sleep Apnoea Trust
It is not possible to place a time constraint on the aims of our group, mainly because situations change, particularly in the medical field. The nub of our aims is to deliver both tangible benefits and relevant information to our members, to improve facilities and resources (within Ireland) for the diagnosis and treatment of Sleep Apnoea and to assist in or fund research into the disorder.
To achieve this ISAT will:
Promote and Advocate awareness of Sleep Apnoea
To the Public
To the Medical Profession
To Employer Groups
By way of:
Production of promotional printed and visual materials
Committed positive advocacy
Use of all local media resources
Use of all national media resources
In partnership with the Department of Health and Children/Health
Provide support to medical, nursing and treatment services
By way of:
Identifying specific needs and shortcomings
Being aware of global developments through effective networking with other groups
Being proactive in our approach
Working in tandem with existing services
Encourage the formation and development of an Irish Sleep Society by the medical and technical professionals dealing with Sleep Apnoea in Ireland.
Encourage the Department of Health and Children to introduce a suitable national structure for clinics dealing with Sleep Apnoea.
Develop an effective lobbying body
By way of:
Development of a flexible, effective results driven lobbying plan for Politicians,
Health Insurers, Industry and Legal groups
Use of the Freedom of Information Act and/or Parliamentary Questions
Liaise closely with and form firm links with similar type groups
Develop close links with the media, politicians and state agencies
Chairman/Webmaster: Arthur O'Hara
Vice Chairman: Jim Whelan
Treasurer: Aileen Kavanagh
Assistant Treasurer: Laurence Woods
Honorary Secretary General: Dan Smyth
Chairman of Medical Council: Prof. Walter McNicholas
|With the formation of
The Irish Sleep Society, a group representing all the
medical and technical professionals involved in Sleep Medicine
and Respiratory Sleep Medicine we believe that our Medical
Council will become defunct. The Irish Sleep Apnoea Trust is in
regular contact with the Steering Committee of The Irish Sleep
Society and will develop formal links with it.

domain: health


08 Apr EASYRING
Easyring is an hardness tester specially designed for testing large rings or pipes. This instrument is able to increase the productivity of tests on rings thanks to the possibility to perform tests on walls inclined at 90° without having to move the piece. The machine is fully adjustable with automatic cycles to carry out tests on the 2 outer faces of the ring. (Horizontal and Vertical).


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We didn’t endorse Saraki, Mohammed as consensus candidates – Northern elders
The Northern Elders Forum (NEF) has refuted reports that it has thrown its weight behind former Senate President Bukola Saraki and Bauchi State Governor Bala Mohammed as consensus presidential aspirants of the People’s Democratic Party (PDP) from the region.
Recall that there were plans by Saraki, Mohammed, Governor Aminu Tambuwal of Sokoto and Mohammed Hayatudeen to work towards a consensus presidential candidate from the north.
However, Mohammed and Saraki were announced as the northern consensus presidential aspirants of the PDP by Prof. Ango Abdullahi on Friday.
The development has continued to generate reactions as major stakeholders and the aspirants who were initially part of the consensus arrangement make their position known.
Tambuwal had since dissociated himself from the outcome, declaring that the consensus plan had failed, while former Jigawa State Governor Sule Lamido described it as a charade and unacceptable.
Former vice president, Atiku Abubakar, and Mohammed Hayatudeen have since denied being a party to the arrangement.
Reacting to the development, Dr Hakeem Baba-Ahmed, NEF Director of Publicity and Advocacy, the northern elders were not involved in the process that threw up Saraki and Mohammed as northern consensus candidate.
In a statement, he said the attempt to engineer a consensus among the aspirants was that of the aspirants themselves.
Baba-Ahmed said, “Governors Aminu Tambuwal and Bala Mohammed, former Senate President Bukola Saraki and Mohammed Hayatudeen had informed for head of state, Badamasi Babangida, of their intention, and requested elderly intervention and advice in the event that their efforts failed to produce consensus.
“Babangida in turn requested Prof. Ango Abdullahi in his individual capacity to design and manage a process that could improve a consensus agreement among the four aspirants. Prof. Abdullahi concluded that assignment and released a detailed report and recommendation, signing it in his individual capacity,”
He said the entire process did not involve the Northern Elders Forum as a group, and was not a product of the group.
Professor Ango Abdullahi, he said, was free to undertake any lawful activity as a citizen, and genuine misinterpretations of his role are understandable.
The post We didn’t endorse Saraki, Mohammed as consensus candidates – Northern elders appeared first on NEWS.

domain: politics


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- 1 Who influenced Tina Turner?
- 2 What is Tina Turner’s net worth?
- 3 Did Tina Turner mother leave her?
- 4 Why did Craig Turner die?
- 5 Is Tina Turner in Rock and Roll Hall of Fame?
- 6 How much is Halle Berry worth?
- 7 What is Diana Ross’s net worth?
- 8 How much is Beyonce worth?
- 9 What happened between Ike and Tina?
- 10 How is Tina Turner doing now?
- 11 What is the tragic news about Tina Turner?
- 12 How much older is Tina Turner than her husband?
- 13 What did Craig Turner do for a living?
Who influenced Tina Turner?
3. Janet Jackson. Powerhouse performer Janet Jackson lists Tina as a massive influence and inspiration for her, both personally and professionally.
What is Tina Turner’s net worth?
Tina Turner’s net worth in 2021 is estimated to be anywhere between $250 million and $270 million, according to multiple net worth calculators.
Did Tina Turner mother leave her?
When Anna-Mae is 11, her mother Zelma leaves her abusive relationship with Tina’s father and moves to St Louis. Her father Richard remarries and leaves Anna-Mae in the care of her grandmother.
Why did Craig Turner die?
At the time, the Los Angeles County Department of Medical Examiner-Coroner told People that Craig Turner was found dead in his California home from a self-inflicted gunshot wound. He was age 59.
Is Tina Turner in Rock and Roll Hall of Fame?
The incomparable Tina Turner celebrates her birthday today (November 26.) While she has received numerous accolades throughout her incredible career, the fact she hasn’t been inducted into the Rock and Roll Hall of Fame for her solo work is absurd.
How much is Halle Berry worth?
Introduction. As of 2021, Halle Berry’s net worth is $90 million. Halle Berry is one of the most iconic and successful actresses in Hollywood.
What is Diana Ross’s net worth?
According to reports, Ross’s net worth sits at a cool $250 million. She’s been a highly valued singer since the earlier days of her solo career. After hitting it big with The Supremes in the 1960s, Ross split with her group and made her final performance with them in January 1970.
How much is Beyonce worth?
Superstar Beyoncé Knowles has a net worth of $420 million, according to Forbes. She earned $81 million in 2019 and ranked as one of the world’s highest-paid celebrities.
What happened between Ike and Tina?
In Dallas in 1976, she escaped her husband and found safety at a Ramada Inn. She fled after a fight with Ike left her bloodied, and she arrived at the hotel with barely any money. After living through Ike’s “torture” for 16 years (as she described it to People in ’81), Turner (then 37) divorced the musician in 1976.
How is Tina Turner doing now?
She lives in Switzerland with her husband. Turner and Bach, whom she married in 2013, share an idyllic home (called the Chateau Algonquin) in Zurich, Switzerland.
What is the tragic news about Tina Turner?
Such phrasing could worry Turner’s fans given her age and health woes over the years. She suffered a stroke in 2013, was diagnosed with intestinal cancer in 2016 and considered assisted suicide before her husband donated a kidney for transplant surgery in 2017.
How much older is Tina Turner than her husband?
Tina met Erwin Bach in 1985 at an airport reportedly and they soon fell in love. Their 16-year age difference (Bach is younger than Tina ) didn’t stop the couple from taking their relationship to the next level.
What did Craig Turner do for a living?
Craig was working in real estate at the time of his death. The October after Craig’s death, Tina told BBC News that he seemed fulfilled, with a new job and a new girlfriend in his life. (According to ET, Craig worked in real estate in California’s San Fernando Valley.)

domain: celebrity


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Skip to Main Content
The aim of this paper is to discriminate liver diseases from CT images automatically using a sigmoid radial basis function neural network with growing and pruning algorithm (SRBFNN-GAP). We develop a novel SRBFNN-GAP to discriminate cyst, hepatoma, cavernous hemangioma, and normal tissue using gray level and Gabor texture features. The proposed SRBFNN adopts sigmoid function as its kernel because the sigmoid function provides a more flexible shape than Gaussian. Furthermore, the GAP algorithm is used to adjust the network size dynamically according to the neuronpsilas significance. In the experiment, the SRBFNN-GAP classifies the features into four classes, and the receiver operating characteristic (ROC) curve is used to evaluate the diagnosis performance.


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Japan's Aerospace Exploration Agency (JAXA) announced Wednesday that it plans to land an astronaut on the moon by 2030, joining China in a new manned race to space.
It is the first time JAXA has revealed an intention to send Japanese astronauts beyond the International Space Station, and it will mostly likely be part of an international mission, the agency said.
The announcement from Japan Wednesday is just the latest in a series of ambitious space exploration plans by Asian countries, with the increasing competition for space-related power and prestige in the region echoing that of the Cold War space race of the mid-20th century.
In December 2016, China announced plans to land a rover on Mars by 2020 as well as a manned mission to the Moon at some point in the future.
Pictured here is one of JAXA's Mitsubishi Heavy Industries rockets, via Danspace.
On Sunday, the SpaceX capsule containing NASA astronauts Bob Behnken and Doug Hurley safely splashed down in the Gulf of Mexico. (Video above.) It was the first time a private company took humans off-world and returned them to Earth and also the first splash-down of American astronauts in 45 years. At a press conference yesterday, […]
WATCH THE SPLASHDOWN EVENT LIVE HERE, the SpaceX video embed in this post will go live with pre-event content sometime before 7pm EDT on Saturday August 1.
NASA launched its latest and greatest rover, Perseverance, to Mars on Thursday, from Cape Canaveral in Florida.
“The way to have power is to take it.” — Boss Tweed. We think an 18th-century political power broker probably had a different definition of power in mind when he made that statement, yet the sentiment still applies. We’ve all got devices all but falling out of our pockets and the need for power to […]
If the last 50 years of education have taught us nothing else, it’s that it often requires different tactics to best reach different learners. To pick up a foreign language, some students take best to the old-school high school language lab method, using heavy repetition, verb conjugation and grammar emphasis to embed a new language. […]
For those who want a career in video games, there’s no reason to sit around and wait. EA and Rockstar Games probably aren’t going to seek you out and knock on your door with a job opportunity. But if you’re an indie developer with a good idea and some passion, you can create a really […]

domain: aerospace


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Just like the real estate market skyrocketed a few years ago, so did the home-equity lines of credit. But when the real estate market crashed, the HELOC followed the wave right back down. Like home sales, once the economy stabilizes, the HELOC will once again be easily available. While you won’t be able to borrow at as low of rates as once before, it will still help out tremedously. Check out some tips for borrowing a home equity line of credit.
Don’t borrow the max
Most lenders won’t approve a line that brings your total housing debt to more than 80% of your home’s value, and you’ll need a minimum 740 credit score to get that much. But there are good reasons to borrow less. You should aim to keep your total monthly debt payments at no more than a third of your take-home pay. Keep in mind that as the economy recovers, HELOC rates will rise too, so borrow only what you could keep up with if rates jump. If you were to take out a $75,000 HELOC today, for example, you’d owe $344 a month in interest; if rates rise a couple of percentage points the monthly tab will jump to $469.
Use it the right way
By now you almost certainly know that using your home-equity line for frivolities like vacation packages and plasma screens is asking for trouble. Other traditional uses may or may not still make sense:
Home improvements. Tapping your HELOC to fund necessary projects like a roof replacement is still worthwhile: You can deduct interest on up to $1 million when you use HELOC funds to improve a first or second home, which in turn sharply lowers the real cost of the loan. Renovations that won’t necessarily pay for themselves, like a media room or a deluxe kitchen? Take a pass.
Car loans. At a 7.3% rate, a three-year new-car loan costs a lot more than a line of credit. A HELOC can be a good substitute — as long as you expect to pay it back within a few years. You may be able to write off the interest. Though the rules are complicated, in general you can deduct interest on a HELOC for up to $100,000 of non-home-related uses.
Student loans. Max out government-backed Stafford and PLUS loans first. The interest on these loans is usually tax deductible, and they often offer flexible repayment plans. But if you have to take a private loan, a HELOC can be a cheaper alternative.
Small business. Entrepreneurs have long used HELOCs as easy business lines of credit to smooth out bumpy income. Steer clear of that unless you’re confident the business is solid, says Newtown, Pa., financial adviser Jonathan Heller.
Make sure you keep it
If you’re going to use a HELOC as an emergency fund, you have to make sure your line isn’t pulled out from under you. Most banks have stopped freezing existing HELOCs, but that could happen if real estate values drop in your neighborhood. Your best defense is to use your line regularly, even if you take out just $500 at a time. Even during the worst of the credit crisis, issuers weren’t freezing or closing HELOCs that were in use as long as the homeowners weren’t underwater.If you think you’ll need to use your HELOC in a few months and are concerned that it could get chopped, borrow the funds now and park them in an FDIC-insured account to keep them safe. Then start paying the loan back ASAP.
Contact us today: 214.923.0261 or email us: firstname.lastname@example.org


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Get a Charter Bus Quote for New Orleans Motor Coach, New Orleans Mini Bus, New Orleans School Bus, New Orleans Entertainer Coach, Tour Bus, Limousines, New Orleans Executive, New Orleans Limo Bus, New Orleans Double Decker, and Passenger Van.
Chalmette Battlefield , as you might be knowing the site of the 1815 Battle of New Orleans — is located just 6 miles east of New Orleans along the Mississippi River. You might be knowing that Chalmette Battlefield displays historical markers helping people trace the history of the Battle of New Orleans. It is here that the national cemetery is also located along with the promptbuscharters.com
Then you can move on to explore the Jean Lafitte National Park. What you will find here is various swamps and bayous, and you might catch a glimpse of an alligator or two. And you cannot miss the numerous cultural events throughout the year as well.
Choose any from the wide choices of Charter Buses, Mini Buses, School Buses, Party Buses, Entertainer, Limousines, Limo Buses, Hummers, and SUVs for your travel to New Orleans City.

domain: geography


In our vineyard, we try to remain as organic as possible and add Biodynamic practices to our viticulture program. So far, if it were not for the one fungal disease, Black Rot, for which there is no organic treatment, we would have been able to maintain an organic vineyard. Even though we cannot apply for organic or Biodynamic certification, we still believe in the benefit of such programs so we apply their farming practices to our vineyard. We try to hold on to our eco-friendly ideal while balancing the reality of the situation and the desire to end up with a crop! The French have a term for this “reasoned struggle,” La Lutte Raisonée. This seems a perfect description for us. The philosophy feels authentic and realistic.
What are the components of an environmentally sensitive vineyard? We emphasize biodiversity by planting cover crops and perimeter flowers, fruit trees and a small vegetable garden in the vineyard grounds, all providing habitat for beneficial insects (and healthy snacks for the workers). We use our sheep at designated times to graze the vineyard floor, thus weeding and fertilizing our low-nitrogen soils. We add Biodynamic compost to the soil. We have bee hives for pollination and are adding chickens and guinea hens this season to feast on the insect pests. Bluebird houses and Martin houses are there to help with the bugs as well. We keep our conventional sprays to a minimum and then use only the “softer” of the sprays to combat Black Rot. We also know that keeping the vineyard clean and proper leaf canopy management is essential to keep the air moving around the leaves and grapes, thus lessening the fungal disease pressures.