text: Data Availability StatementThe datasets generated and analyzed through the present research aren’t publicly available because of further research getting performed, but can be found in the corresponding writer on reasonable demand. amounts. Notch1 was defined as a direct focus on of miR-145-5p. Furthermore, cleaved caspase-3, Bcl-2 and Bax amounts were reduced by miR-145-5p overexpression significantly. These results indicate that miR-145-5p overexpression inhibited the Notch signaling podocyte and pathway lesions induced by HG. In conclusion, the full total benefits of today’s research recommended that miR-145-5p could be a regulator of DN. Additionally, miR-145-5p inhibited HG-induced apoptosis by concentrating on Notch1 and dysregulating apoptotic elements straight, including cleaved caspase-3, Bcl-2 and Bax. The outcomes of today’s research provided proof that miR-145-5p may provide a book approach for the treating DN. luciferase activity. Statistical evaluation All experiments had been repeated 3 x and analyzed using GraphPad Prism 5.1 software program (GraphPad Software, Inc.). Data are portrayed as the means Rabbit Polyclonal to B-Raf SD. Statistical analyses had been completed using one-way ANOVA accompanied by Tukey’s multiple evaluation post hoc check. P 0.05 was considered to indicate a significant difference statistically. Outcomes Appearance degrees of miR-145-5p are reduced in HG-induced podocytes First, in order to investigate the role of miR-145-5p in HG-treated podocytes, the expression levels of miR-145-5p were detected using RT-qPCR following incubation with 25 mM glucose for 12, 24, 48 and 72 h. As shown in Fig. 1, the expression levels of miR-145-5p were significantly decreased in HG-treated podocytes from 24 h onwards. These data show that miR-145-5p serves a critical role in HG-stimulated podocytes. Open in a separate window Physique 1. Expression levels of miR-145-5p are decreased in HG podocytes. Podocytes were incubated with 5 mM (NG group) and 25 mM (HG group) glucose for different time periods (12, 24, 48 and 72 h). The expression levels of miR-145-5p were measured by reverse transcription-quantitative PCR. Data are offered as the mean SD, and as the fold change relative to the control group (n=3). **P 0.01 vs. NG. miR-145-5p, microRNA-145-5p; HG, high glucose; NG, normal glucose. miR-145-5p expression is usually successfully changed following transfection with miR-145-5p mimic or inhibitor miR-145-5p expression was detected in different transfection groups. Podocytes were transfected with scrambled control, miR-145-5p mimic or miR-145-5p inhibitor for 48 h. Following transfection, the expression levels of miR-145-5p were detected via RT-qPCR. Fig. 2 shows that in the miR-145-5p overexpression group, transfected with miR-145-5p mimic, the expression levels of miR-145-5p were significantly higher compared with those in the scrambled control group. Additionally, in the miR-145-5p knockdown group, transfected with miR-145-5p inhibitor, miR-145-5p expression was significantly decreased in the podocytes compared with control. These data show that this transfection was successful. Open in a separate window Physique 2. Determination of transfection efficiency in different groupings. The appearance of miR-145-5p was assessed by invert transcription-quantitative PCR. Data are provided as the mean SD, so that as the flip change in accordance with the control group (n=3). **P 0.01 vs. the scrambled control. purchase BI6727 miR-145-5p, microRNA-145-5p. Overexpression of miR-145 inhibits HG-induced apoptosis in podocytes Podocyte apoptosis was measured by stream TUNEL and cytometry staining. In stream cytometry assays (Fig. 3A and B), HG-treated podocytes exhibited purchase BI6727 a substantial upsurge in apoptotic cells weighed against the harmful control group. Under HG circumstances, the percentage of apoptotic cells was considerably reduced in the miR-145-5p overexpression group weighed against the scrambled control group, whereas the percentage of apoptotic cells in the miR-145-5p inhibitor group was considerably elevated. In the TUNEL staining assay (Fig. 3C and D), the amounts of apoptotic cells were increased in the purchase BI6727 HG-induced podocytes weighed against NG podocytes markedly. Under HG circumstances, the amount of apoptotic cells was considerably low in the miR-145-5p imitate group weighed against that in the scrambled control group; nevertheless, in the miR-145-5p inhibitor group, the amount of apoptotic cells significantly was.

domain: biology


text:
A letter from our Pastor re: Covid-19
With the concern about the Coronavirus spreading through our communities, our Officers have been discussing ways that we can best serve our membership. Yesterday I received this message from Joey Lee, the Executive Presbyter of the Presbytery of San Jose,
To: The Presbytery of San Jose
After much conversation and actions already being taken by congregations in the presbytery, we believe it is time to suspend public worship, until Easter, April 12. We do not make this recommendation lightly and know it will mean a big disruption in the life of your congregation, but following advice from many public health sources, we believe this is the best course of action at this time. Your session can make decisions regarding other groups that use church property.
Our Session met to consider this recommendation and voted to suspend public worship, until Easter, April 12. Until that time, each week you will be able to access the worship service on the website. This week may be lighter than usual, because we are just beginning the process. Rev. I Sil Yoon will give the sermon this Sunday, March 15.
Go to our website https://www.covenantpresbyterian.net and click on the tab at the top: SUNDAY WORSHIP. Please call the church office or Rev. Boles if you have questions about how to access the online worship opportunities.
Until further notice, ALL of our meetings will take place by conference call or Zoom.
It is our highest priority that we stay connected to one another and we will offer Lenten Studies and Bible Studies online. A phone chain will be developed and you will hear from one of our members on a regular basis. If you would like to be part of this ministry, please let me know.
Each day brings changes to our situation. Our Elders and Deacons will meet regularly to reassess our plans.
At this time Natalie, our office manager, is working from home and the church office. If you need to reach her, it is best to send her an email.
Please be in touch with your friends and neighbors. Call one another. Offer encouragement and support!
Take care of yourself in these days, and don't hesitate to call the office or my cell phone if you have a need.
Come Worship with Us!
Children’s Sunday School:
Children begin in Worship and then
are excused to Sunday School in Room 4
Followed by Coffee Fellowship Hour
in the Fellowship Hall
12:15 p.m. Sundays in Room 8
Adult Christian Education:
12:15 p.m. Sundays in the Fireside Room
The Covenant Community
Welcome to Covenant
Worship Sunday at 10:30 AM
Our Church Family is
Faithful, Lively, Diverse, and Unique!
Our life together is defined by
We are God’s Children!
Serving Christ guided by the Holy Spirit.
We are part of the
Presbyterian Church (USA).
Monday – Friday (except Wednesday)
10:00 a.m. – 2:30 p.m.
Wednesday – 10:00 a.m. – 2:00 p.m.

domain: christianity


text:
Kieran Payne asked for a valuation on the coin pictured here. It’s not really in my line, as it is a brilliant uncirculated gold £5 piece, dated 1991 and in a presentation case. The coin was purchased from Harrods on the 31st of August 1991 for £535. Kieran still has the receipt and paperwork that came with the coin back in 1991. It was numbered 0499 of 1500 but the total mintage was only 976.
Price of gold
When it comes to the value of coins like this one much depends on the price of gold on the international market. It is priced in US$ and can go up or down. When there is a crisis of some kind somewhere in the world the price usually rises; when things are stable the price often drops.
Up to the 1960s the gold price was fixed at 35$ per ounce. The price was stable because the USA guaranteed to supply gold at that figure. However, speculators entered the market and a point was reached where the USA couldn’t afford to continue to supply gold at 35$ per ounce. Therefore, the price was allowed to float and it immediately went over the 35$ that it had previously been fixed at. Since then it has continued to rise but there have been times when it has dropped. When Gordon Brown was in charge of our finances he decided to sell our gold reserves. This was a curious move, for at the time the gold price was lower than it had been for some time.
Some people buy gold as an investment but great care needs to be taken. The international price is still fixed in US$, so buyers in the UK need to pay attention to two things: the gold price and the strength of the £. Both these factors have an impact on what profit or loss can be made.
When I first became really interested in coins I lived for a time in Welwyn Garden City. One of our neighbours was a bullion dealer in London and he invited me round to see some of his coins. During the course of business he had bought several sets of gold and silver coins. They included sets dated 1887, 1893, 1902 and 1911. They had originally contained £5, £2, sovereign and half sovereign but only the silver coins were still in place; all the others had been sold for their weight in gold. At the time an 1887 full proof set would have been worth around £120 but today, in mint state, the price would be around £30,000!
Don’t sell it at auction
Under no circumstances should a modern £5 piece be sold through an auctioneer. The latter might offer to sell and charge no commission. However, a buyer at auction will know about the extra premium that is added to the hammer price, so would knock that off his or her highest bid; in that case you would end up with less than the melt value of a gold coin.
Coins as an investment
Finally, unless you know the market inside out then never buy coins as an investment. This applies in particular to the ever-growing number of individual coins and sets that seem to appear on the market on an almost a daily basis. Many are worth nothing more than their weight in scrap metal. I know of people who have built up large collections, supposedly as an investment for their grandchildren. However, when the grandchildren come to sell these collections they will be lucky to receive half of what their grandparents paid for them.
Having said all that, a 1991 brilliant uncirculated £5 piece isn’t really in my line, I did check out prices and one source had a specimen on sale at £1,800. If Kieran wanted to sell his coin then I would advise him to check prices that bullion dealers were offering for similar coins. Bullion dealers unusually work on a much narrower profit margin than coin dealers so they would be likely to offer the highest price.
Coin Valuation Service
Have your coin or artefact valued using my free online coin valuation service

domain: finance


Keppel’s KFELS B Class jackup, with 34 rigs in operation and another 19 on order, are considered the ‘go to’ jackup amongst drillers
A consortium led by Keppel Kazakhstan and partner Ersai Caspian Contractor said Tuesday that they have secured a contract from a subsidiary of Kazakhstan’s state-owned oil company, KazMunayGas, for a Keppel KFELS B Class jackup rig worth US$242 million.
The rig, once completed in 2015, will help tap Kazahkstan’s vast oil reserves in the Caspian Sea that are estimated to hold some 30 billion barrels oil and 85 trillion cubic feet natural.
Today’s announcement marked both good news for Kazahkstan’s oil and gas resurgence and Keppel’s dominance in the jackup rig market.
“Together with our consortium partner, we at Keppel look forward to delivering a high quality rig to Teniz Burgylau safely, on time and within budget” said Chow Yew Yuen, CEO of Singapore-listed Keppel Offshore & Marine, which is a 50% shareholder of Keppel Kazakhstan. “With 34 rigs in operation and another 19 on order, the KFELS B Class design has been the rig of choice for drilling.”
Meanwhile Nurlan Bekzhanov, General Director of Teniz Burgylau commented: “Kazakhstan aims to steadily increase its oil and gas production to become one of the world’s top oil and gas producers and exporters. Teniz Burgylau is grateful to its shareholders KazMunayGas and KazMunayTeniz for the given opportunity to contribute towards this target by stepping up our drilling operations. For our rig, we have decided on the KFELS B Class jackup design as it is an industry benchmark for such rigs. With the established track record of Keppel O&M as the world’s leading offshore rig designer and builder, and its presence in Kazakhstan, we believe we have chosen the right partners to provide our first jackup drilling rig.”
Keppel Kazakhstan’s track record of projects include the fabrication of a series of Pipe Rack Modules, Pontoons, Hook Up Structures and the building of seven Ice Class Self Ballasting Transportation Barges for the Kashagan field development. Keppel Kazakhstan is also the first yard to launch a steel floating vessel into the Caspian Sea in Kazakhstan.
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text:
What It’s Like to Prepare Cleveland for the Republican Convention
Brittany Williams talks about getting ready for one of the biggest national political events just four weeks after the city won an NBA championship.
The Republican National Convention in Cleveland, Ohio, has proved more than eventful. Amid allegations of Melania Trump plagiarizing her speech, the absence of notable figures in the party, protests in the city, and a shouting match on the convention floor—a nominee emerged. Donald J. Trump was (finally) formally confirmed as the party’s candidate for the presidency.
But while the event has provided plenty of political drama, it’s been mostly smooth sailing logistically. For The Atlantic’s series of interviews with American workers, I spoke withBrittany Williams, the senior project manager for the Cleveland 2016 Host Committee, who knows all of the preparation that it took to get Cleveland ready for the influx of 50,000 people into a city that normally houses almost 400,000. Some members of her team have been working for two years coordinating everything from welcome events to traffic patterns to security for the city. The interview that follows has been lightly edited for length and clarity.
Adrienne Green: How did you get this job and how long in advance did you start working on the convention that’s going on this week?
Brittany Williams: I was brought on as the senior project manager of the Host Committee in May of 2015. My background is actually in politics, prior to this position. I was the executive director for the Republican Party of Cuyahoga County, and also did fundraising and worked in the treasurer's office. In 2014, I was a delegate to the Tampa convention. A lot of [the reason I was hired] was being able to navigate both worlds. So this job was perfect, because I'm getting to work with all of those civic partners while still working on events that are still political—even though we aren’t coordinating the political programming. I understand the RNC; I understand the players; I understand the structure; I understand and know who's doing what and why.
Green: And so obviously this is not your first convention. How is it different than when you went in 2012?
Williams: It’s definitely different being on this side of the fence. When you're a delegate, everything is presented to you: Your responsibility is to just show up, vote, and listen to speakers. It’s like when a meeting is prepared for board members and everything's there for them to read, versus being the one who has to get everything together for the people attending the meetings.
It’s amazing to see how much work goes into the convention: having to work on transportation, security, and beautification projects. We had volunteers that were out hanging up more than a million strands of twinkle lights just to make the city prepared and look beautiful for everyone, in addition to fixing roads and reworking bus routes to prepare for 50,000 people to come into the city.
Green: Were there any delays?
Williams: The Cleveland Cavaliers went to the very last game of the NBA finals. Normally, they usually have six to eight weeks to flip an arena into a convention hall. They had about four for this one. As soon as the Cavs left the final game in Cleveland, then they went back to Golden State. [RNC Organizers] had to get in there, and in 48 hours they pretty much stripped the entire floor and the first row seats.
In terms of coordinating, [RNC organizers] had to touch base with each of the 5,000 delegates and alternates and make sure their travel arrangements were made.* On top of all the media coming in, it's fascinating to just see all of the different players, all of the people that are working on the project. There was a countdown clock for when the convention starts. On Sunday night, it was a little surreal because at one point there would be a year on it, then there were multiple days left. I walked by at one point on Saturday night it was down to 23 hours. That realization that everything was going to happen in less than a day, and we had to be ready.
Green: Has all of the work that you’ve done been for the Republican Party?
Williams: I have always worked for the Republican Party, yes. The first job I did was volunteering with my mom. She would always volunteer in the community, and it was never really for a political party. And then I went to college and started streamlining my own political thoughts. When I graduated from grad school, I moved home to Cleveland and I was job hunting. While I was in the middle of my job search, I started volunteering for a local candidate—a municipal court judge. Her campaign manager was involved in the Republican Party.
Green: What's the vibe at the convention and in Cleveland? This year, it appears that there's tension within the Republican Party and the convention is kind of the centerpiece of that.
Williams: There may be tensions and things going on at the political level, but on the streets the city is electrified. It's fully alive. If there's tension in the convention hall there's not tension on the streets at all.
Our job first and foremost is to make sure that the city of Cleveland is prepared to host. And our job right now is to make sure people are happy to be here and that people want to come back.
Green: What’s a typical day like for you?
Williams: No day is the same. I've had to oversee a variety of projects: Working on everything from signage and billboards around downtown, to all of the gift bags that went out to the delegates, the media, and the sponsors. I've never had to coordinate the assembling and delivery of 22,000 gift bags before, but managed to pull that off. So a lot of it is troubleshooting. I’m kind of the chief firefighter for a lot of things, but just making sure that the ball is constantly moving forward.
Sunday was a major day for us because we had that welcome event and it was the main day a lot of delegates arrived in Cleveland. People are coming in, and they don't know where to go. They need help finding their hotels and navigating the city. The actual formal first day of the convention was hectic as well. Cleveland has never had an event of this size, and it definitely never had an event with the security parameters needed to keep everyone safe.
Green: What are your hours like now that we're kind of in the midst of the convention?
Williams: My hours are definitely not set. If something needs to be accomplished, it doesn't matter what time of the day it is. But I've always thrived in that sort of environment. This what it's like to work on a political campaign. I mean, when you're on a campaign, election day is coming. And if you're not ready, it's going to be a bad outcome. So you have to leave it all out on the table, and there is no time to sleep and there's no time to really second guess.. In April or May, it really switch to no breaks, working seven days a week. Now the convention is the RNC’s; it's their show to run. So in addition to all of the background work behind the scenes, that’s what gets on the TV screen but without [the prep work] you wouldn't be able to effectively run the convention.
Green: Do you have any plans for where you're going next?
Williams: Having come from campaign world, I definitely want to stay in Cleveland. I am a German Marshall Fund fellow, so I will be in Europe studying transatlantic relations from October through November. I always get asked this question of, “what are you going to do afterwards?” It's just like working on a campaign again. Come November, any campaign staffer knows that after the election you either [going to] have a job or you're not. For the hand that loses, that's the end of the road. So that sort of stress doesn't get to me; the most immediate plan that I have is a nice vacation.
Green: What has doing your job taught you about what it means to work?
Williams: My parents used to always tell me that you shouldn't live to work, but work to live. And when you have a job that you love, that you feel passionate about, it's not really work at all. I always aim to be in a position where I'm able to give back, and I think that's what has drawn me to politics. Being able to make change in my community for the better, I believe those changes are fully made through our political system. So for me, working on campaigns and working on events, it's promoting my home. It's trying to do the best that I can to draw attention to Cleveland.
*This article originally misstated that it was Williams and her committee who were responsible for contacting delegates before the convention. We regret the error.
This interview is a part of a series about the lives and experiences of members of the American workforce, which includes conversations with a government agency director, a state representative, and a lawyer.

domain: politics


Geological carbon dioxide sequestration
Geological carbon dioxide sequestration (in Energy; a geoscience perspective, Allison M. Macfarlane (editor))
Elements (June 2007) 3 (3): 179-184
A key means of reducing greenhouse gas emissions from fossil fuels is to separate and concentrate CO (sub 2) from large point sources and inject it underground. The injection process, so-called "geological carbon sequestration", uses off-the-shelf technology from the hydrocarbon industry and can be deployed at a useful scale. Widespread deployment will require a greater understanding of processes that trap CO (sub 2) underground, improved means of monitoring the injection stream, and a small number of large-scale experiments in settings with the most important representative geology. If successful, geological sequestration could greatly reduce greenhouse gas emissions while we continue to benefit from fossil fuels until true alternatives emerge.


text:
Nick Cave '20,000 Days on Earth’ First official clip.
Sundance film festival victories
Nick Cave, an Australian songwriter, poet, composer, and screenwriter. And now actor, sort of. The film '20,000 Days On Earth' has already won best Directing, and Editing awards this year at Sundance, and has wowed fans and critics alike at its preview screenings.
The synopsis for the film reads; “Drama and reality combine in a fictitious 24 hours in the life of musician and cultural icon Nick Cave. With startlingly frank insights and an intimate portrayal of the artistic process, the film examines what makes us who we are and celebrates the transformative power of the creative spirit.”
If the reviews and accolades are mounting up before its release, it may well deliver. And is Nick Caves track record is anything to go by, I think we can all sleep easy in knowladge that it will. Screening at various film festivals until its worldwide release later this year. You can view the website here and watch the first official clip below.

domain: movie


text:
An RIL population consisting of 304 lines was constructed from the indica
cross Zhong 156/Gumei 2. Two genes conferring complete resistance to blast
with isolate 92-183 (ZC15) were mapped (Zhuang et al., 1997, Zhuang
et al. 2002). One of the genes, Pi-25 (t) on chromosome
6, conferred resistance to both leaf blast and neck blast. The other gene,
Pi-24 (t) on chromosome 12, conferred resistance to leaf blast
only. Seventy-four RILs that did not possess the resistance allele of
Pi-25 (t) based on flanking markers and were susceptible to isolate
92-183 for neck blast, were selected for mapping genes conditioning partial
resistance to neck blast with 92-183. The heading dates of these lines
Both isolates 99-30-1(ZB1) and Ca89 (lineage 4 in the Philippines) were
compatible to Zhong 156 and incompatible to Gumei 2 for leaf blast. One
hundred and sixteen RILs and one hundred and forty-six RILs susceptible
to isolates 99-30-1 and Ca89 respectively, were chosen to analyze partial
resistance to leaf blast.
Diseased leaf area (DLA), lesion size (LS) and lesion number (LN) were
measured as parameters of partial resistance to leaf blast. Lesion length
(LL) and the conidium amount (CA) were recorded as parameters for QTL
mapping of neck blast resistance.
As shown in Fig. 1, 62 QTLs scattered in 28 intervals of the 12 chromosomes
were detected using LOD >= 2.4, of which 11 QTLs showed main effects
only, 47 QTLs showed epistatic effects only and four showed both main
and epistatic effects. Fifty-one QTLs showing epistatic effects were involved
in 28 pairs of significant interactions between QTLs. General contributions
of QTLs showing epistatic effects of each parameter ranged from 16.08%
to 51.70%, while those of QTLs showing main effects of each parameter
ranged from 5.04% to 42.2%. The general contributions of QTLs showing
main effects of most parameters were smaller than that of QTLs showing
epistatic effects, confirming the importance of epistasis as the genetic
basis for complex traits. The general contributions of the main plus epistatic
effects of all QTLs detected for the two parameters for neck blast reached
74.0% and 74.6% of trait variance, respectively, which obviously represented
a major part of the genetic basis controlling partial resistance at reproductive
stage. In nine out of the 28 intervals, QTLs were detected for parameters
of both leaf and neck blast resistance, indicating that the genetic bases
for partial resistances were related but quite different for different
isolates and at different growth stages. For the seven out of 15 QTLs
with main effects, resistance alleles were from the susceptible parent.
A great number of genes with complete or partial resistance to blast in
rice have been mapped so far, which included Pi1(t), Pi2(t),
Pi4(t), Pi5(t), Pi6(t), Pi9(t), Pi10(t)
and Pi11(t) (Causse et al.,1994), Pi5(t) and Pi7(t)
(Wang et al., 1994), Pia, Pib, Pik, Pit,
Pita, Pi12(t), Pi17(t), Pi18(t), Pi19(t),
Pi20(t), Pi23(t), Pi62(t) and Pi157(t) (Nagato
and Yoshomura, 1998), Pitq1, Pitq5, Pi-tq6 and Pilm2
(Tabien et al., 2000), the recessive gene pi21(t) (Fukuoka
et al., 2001), the QTLs for partial resistance to leaf blast (Wang
et al., 1994) and QTLs for partial
resistance to neck blast (Bagali et al., 2000). The comparison
of locations of the major genes and QTLs mapped in this population with
reported resistance loci for complete and partial resistance established
the association of blast resistance loci in rice (Fig. 1). Clusters of
blast resistance genes are more obvious in the rice genome. The coexistence
of major genes and QTLs also provides evidence to support the hypothesis
that QTLs and major genes were different alleles of a same locus and qualitative
mutant alleles that affected quantitative traits represented one extreme
in a spectrum of alleles (Robertson 1985).
This work was funded by Chinese 863 program (2001AA211081), Asian Rice
Biotechnology Network and the Rockefeller Foundation International Rice
Bagali, P. G., S. Hittalmani, S. Y. Shashidhar and H. E. Shashidhar, 2000.
Identification of DNA markers linked to partial resistance for blast disease
in rice across four locations. In: D. Tharreau, M. H. Lebrun, N. J. Talbot,
and J. L. Notteghem (eds), Advances in Rice Blast Research. Kluwer Academic
Publishers, Dordrecht, Netherlands. p. 34 -44.
Causse, M. A., T. M. Fulton, Y. G. Cho, S. N. Ahn, J. Chunwongse, K. S.
Wu, J. H. Xiao, Z. H. Yu, P. C. Ronald, S. E. Harrington, G. Second, S.
McCouch and S. D. Tanksley. 1994. Saturated molecular map of the rice
genome based on an interspecific backcross population. Genetics, 138:
Fukuoka, S. and K. Okuno. 2001. QTL analysis and mapping of pi21,
a recessive gene for field resistance to rice blast in Japanese upland
rice. Theor. Appl. Genet., 103: 185-190.
Nagato, Y. and A. Yoshimura. 1998. Report of the committee on gene symbolization,
nomenclature and linkage groups. RGN, 15: 13-74.
Robertson, D. S. 1985. A possible technique for isolating genic DNA for
quantitative traits in plants. J. Theor. Biol., 117: 1-10.
Tabien, R. E., Z. Li, A. H. Paterson, M. A. Marchetti, J. W. Stansel and
S. R. M. Pinson. 2000. Mapping of four major rice blast resistance genes
from 'Lemont' and 'Teqing' and evaluation of their combinatorial effect
for field resistance. Theor. Appl. Genet., 101: 1215-1225.
Wang, G.-L., D. J. Mackill, J. M. Bonman, S. R. McCouch, M. C. Champoux
and R. J. Nelson. 1994. RFLP mapping of genes conferring complete and
partial resistance to blast in a durably resistant rice cultivar. Genetics,
Zhuang, J.-Y., R.-Y. Chai, W. B. Ma, J. Lu, M. Z. Jin and K. L. Zheng.
1997. Genetic analysis of the blast resistance at vegetative and reproductive
stages in rice. RGN, 14: 62-64.
Zhuang, J. Y., W. B. Ma, J. L. Wu, R. Y. Chai, J. Lu, Y. Y. Fan, M. Z.
Jin, H. Leung and K. L. Zheng. 2002. Mapping of leaf and neck blast resistance
genes with resistance gene analog, RAPD and RFLP in rice. Euphytica, in

domain: agronomy


The Earth Institute is grateful to its many partners for their important role in the effort to develop the science and solutions necessary for sustainable development. Please visit the interactive digital 2013 Annual Report to read more about how we are forging partnerships across disciplines and sectors to advance the global effort to guide our planet onto a path toward sustainability.
The Earth Institute Center for Environmental Sustainability (EICES) at Columbia University invites you to enroll in courses this Spring via our Certificate Program in Conservation and Environmental Sustainability.
Earth Institute research expeditions investigating the dynamics of the planet on all levels take place on every continent and every ocean. Most projects originate with our main research center, Lamont-Doherty Earth Observatory, and are often run in collaboration with other institutions.
Are you interested in cultivating the skills necessary to implement environmental change? Do you want to learn more about conservation and environmental sustainability, including ecosystem services and function?
Watch a slide show featuring ongoing research by scientists at Columbia University’s Lamont-Doherty Earth Observatory, whose work around the globe is key to understanding past changes in the oceans and what is going on today.
Much of the modern understanding of climate change is underpinned by pioneering studies done at Columbia University’s Lamont-Doherty Earth Observatory. Here’s a timeline of significant studies.
In an article published in The Lancet, Earth Institute Director Jeffrey Sachs outlines his own ideas for sustainable development goals, and how how these goals can build on the Millennium Development Goals, the UN’s set of targets that aim to reduce extreme poverty and boost social well-being in many other ways by 2015.
Leading up to Rio+20, on April 25th the United Nations hosted “Healthy Oceans: Charting A New Course,” a panel discussion which brought together a range of experts to discuss the fate of the world’s oceans and what can be done to protect them.
A new study in Science finds that the oceans may be acidifying faster today from industrial emissions than they did during four major extinctions in the last 300 million years when carbon levels spiked naturally.


Why is My HP Printer Won’t Connect to WiFi?
Is your HP printer won’t connect to WIFI? HP is a well-established company best-known for selling some of the best quality printers in the market.
However, many times the users complain about experiencing HP printer won’t connect to wifi problem.
Undoubtedly, a printer that is unable to connect to the wifi is one of the most frustrating issues. But, it is a common problem that hounds most printer users.
If you are having a hard time connecting your HP printer to the wifi then you are at the right place. Here we are going to discuss in detail the solutions that can help you fix this issue.
Basic Things you Need to Check if HP Printer Won’t Connect to WiFi
Before knowing the troubleshooting solutions there are a few basics that you need to check while following the steps.
- Check the settings of the router.
- Disable the VPN connection.
- Reset the printer.
- Uninstall and reinstall the driver of the printers.
- Restart all of the devices.
- Make sure to check if the printer power is turned on.
- Check the toner, ink, and paper tray.
- Use a USB cable connection and check if it connects or not.
Troubleshooting Your HP Printer Won’t Connect to WiFi
If you are having trouble connecting the HP printer to the wifi then do not worry. Here are some of the solutions that you can try to resolve the problem on your own.
1. Set the Printer as Default: Sometimes while trying to connect to the wi-fi the printer may be set as default. Here are the steps that you need to follow.
- Click on the Start menu and then select the Control Panel.
- Then choose the Hardware and Sound option.
- Choose Devices and Printers.
- Right-click on the printer then select the option to ‘set as default’.
- Click on the yes option to confirm.
2. Restart All of the Device: A quick restart can often help in resolving issues like HP printer won’t connect to wifi.
You need to restart the computer, printer, and router to fix the wi-fi connectivity issue. Simply unplug the devices, and then wait for a minute. Plugin and check if the problem is fixed or not.
3. Run the Printer Troubleshooter: Often running the printer troubleshooter can help in resolving the wifi connectivity issues. Here is how you can run the printer troubleshooter.
- Click on the Control Panel.
- Then choose the Hardware and Sound and select Devices and Printers.
- Right-click on the printer and then troubleshoot.
- The printer troubleshooter will resolve will identify the problem and fix the problem.
4. Check the Router Settings: If you have recently changed the router settings then reconfiguring the computer and printer to connect to the network can fix the HP printer won’t connect to wifi problem.
Here are the steps that you need to follow.
- Open a browser and enter the IP address for the router.
- Under the wireless or security section change the settings to default.
5. Get Help from the Experts: If the solutions mentioned above are unable to fix HP printer won’t connect to wifi then make sure to seek help from the experts.
Get in touch with the technical support team for more information and step by step assistance.
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text:
Excessive use of N fertilizers in agriculture often leads to NO3− accumulation in the unsaturated zone and to groundwater pollution. There is uncertainty regarding the variability in fertilizer transport and uptake efficiency due to the lack of studies based on continuous nondestructive measurements in unsaturated soils. In this study, we analyzed solute dynamics across the unsaturated zone underlying cultivated agricultural fields. Commercial crop rotations under four treatments, comprising two N fertilization regimes and two irrigation water salinity levels, were conducted in loess soil in the semiarid climate of the northern Negev Desert, Israel. The impact of the various treatments on water and solute dynamics below the root zone was monitored by a vadose zone monitoring system. The patterns of variations in soil water content and solute concentrations were analyzed using nonnegative tensor factorization. We found that irrigating using higher salinity water resulted in the earlier arrival of wetting fronts to the deeper layers and increased NO3− concentrations relative to the lower salinity treatments. Surprisingly, this effect was only seen in the deeper soil levels, whereas there was no significant difference in the arrival times and concentrations in the upper soil layers. Possible mechanisms are suggested and discussed.
ASJC Scopus subject areas
- Soil Science


text:
|시간 제한||메모리 제한||제출||정답||맞은 사람||정답 비율|
|1 초||512 MB||7||2||2||28.571%|
Having stocked up with snowballs, Zenyk and Marichka already wanted to start a game.
But suddenly, Zenyk thought that throwing snowballs on a flat surface is boring. He wanted to build as high as possible hill for himself to climb at it and throw snowballs at Marichka.
Building of a hill isn’t easy. Zenyk treated it seriously, took a sheet of paper with coordinate axes, where y-axis is directed upwards, and began to draw the cross section of a hill (a front view).
Marichka doesn’t want the hill to be too high, so she imposed some constraints at its section.
Zenyk wants to know the maximum height he of a hill he can make under these constraints, and asks you the maximal y-coordinate of the hill’s section. Help him find it.
The first line contains four integers x0, y0, xn, yn (|x0|, |y0|, |xn|, |yn| ≤ 106) – coordinates of start and end of the chain.
The second line contains an integer n (1 ≤ n ≤ 105) — number of segments in the chain.
The third line contains n integers l1, . . . , ln (1 ≤ li ≤ 106) — lengths of the segments.
If there is no hill that satisfies these constraints, output “IMPOSSIBLE”.
Otherwise, output one real number — the maximum y-coordinate of the highest point. The answer will be considered correct if its absolute or relative error doesn’t exceed 10−7.
2 3 8 3 2 5 5
4 7 44 77 4 4 7 7 4

domain: mathematics


text:
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|Therapy Name||ADC ST8176AA1|
|Drug Name||Trade Name||Synonyms||Drug Classes||Drug Description|
|ADC ST8176AA1||Trastuzumab-HDACi ST8176AA1|ST8176AA1 ADC|ST8176AA1-ADC||HER2 (ERBB2) Antibody 47||ADC ST8176AA1 is an antibody-drug conjugate comprising Herceptin (trastuzumab), an ERBB2 (HER2) antibody, linked to ST7464AA1, which delivers the HDAC inhibitor to tumor cells expressing ERBB2 (HER2), potentially inhibiting Erbb2 signaling, and resulting in increased DNA damage and apoptosis, and reduced cell proliferation and tumor growth (PMID: 32039017).|
|Molecular Profile||Indication/Tumor Type||Response Type||Therapy Name||Approval Status||Evidence Type||Efficacy Evidence||References|
|ERBB2 positive||colon carcinoma||sensitive||ADC ST8176AA1||Preclinical - Cell line xenograft||Actionable||In a preclinical study, ADC ST8176AA1 treatment reversed epithelial to mesenchymal (EMT) transition, induced DNA damage, increased apoptosis, and inhibited Erbb2 (Her2) signaling and proliferation of ERBB2 (HER2) expressing colon carcinoma cells in culture, and increased median survival in a cell line xenograft model (PMID: 32039017).||32039017|
|ERBB2 over exp||pancreatic carcinoma||sensitive||ADC ST8176AA1||Preclinical - Pdx||Actionable||In a preclinical study, ADC ST8176AA1 treatment inhibited tumor growth in patient-derived xenograft (PDX) models of pancreatic carcinoma overexpressing ERBB2 (HER2) (PMID: 32039017).||32039017|
|ERBB2 positive||ovarian carcinoma||sensitive||ADC ST8176AA1||Preclinical - Cell line xenograft||Actionable||In a preclinical study, ADC ST8176AA1 treatment induced apoptosis and inhibited proliferation of ERBB2 (HER2) expressing ovary carcinoma cells in culture, and inhibited tumor growth, and increased median survival in cell line xenograft models (PMID: 32039017).||32039017|
|Clinical Trial||Phase||Therapies||Title||Recruitment Status|


Sydney home seekers in some of the city’s most popular suburbs could soon get some respite from one of the worst listings shortages on record.
Westpac and Insight Data solutions research showed homeowners in suburbs across the eastern suburbs, northern beaches and Sydney’s west were among the most likely to list a home for sale in the next six months.
It will be a marked change from the current climate, where few homeowners have taken their properties to market, resulting in a listings drought and rapid growth in property prices.
Additional CoreLogic data showed Sydney listing numbers are 21 per cent lower than they were a year ago.
The Likelihood to List ranking measured the average homeowner’s length of ownership and accumulated equity, along with a range of other factors, to determine an owner’s probability of selling.
The research was used to find the top 10 suburbs for house and unit listings across Sydney.
Westpac general manager Will Ranken said an increased intent to sell would make it easier for home seekers.
“We know that lack of supply is a top concern for home buyers navigating the current market,” he said.
“Despite some favourable conditions for buyers, like record-low interest rates, low stock has made it tricky for some to find the right one.
“Our model indicated that certain areas have higher than average tenure and accumulated equity, where longer-term homeowners who have been wanting to sell and move may decide now is the time.”
It was predicted Paddington house owners would be the most likely to sell next year, with house values in the area an average of about $900,000 higher than they were 10 years ago.
Other suburbs expected to record a surge in house sales were Glenmore Park in the Penrith area and Dover Heights, along with northern beaches enclaves Warriewood, Balgowlah, Avalon Beach and Balgowlah Heights.
The house owners most likely to sell in the Sydney’s southwest were those with properties in Ruse and Leumeah.
Apartment owners were expected to be more likely to list if they had homes located in parts of Western Sydney, the north shore or northern beaches.
Unit owners in Blacktown suburb Prospect were rated most likely to list in the next six months, followed by those in Avalon Beach, North Ryde, Toongabbie and Newport.
Rooty Hill in the Mt Druitt area was also expected to see a surge in unit listings.
Asquith and Pymble were forecast to see the most unit owners selling across the north shore.
Westpac research also showed 15 per cent of NSW and ACT resident were considering selling a home in the next five years.


text:
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Cash Connection Sizzling Hot Technical Features
Cash Connection Sizzling Hot is a classic slot that has 5 reels, 3 rows and 5 paylines. The paylines are fixed. The layout is simple with a friendly interface that is easy to comprehend. In this Novomatic review, you will learn how to place your bet on this slot and understand how much you stand to win.
The minimum and maximum amount a player can wager are £0.1 and £50. However, this slot has a maximum payout of 1000x the stake which is an attractive deal. To win the greatest payout, a player has to land five lucky seven symbols in a winning payline. The winning combinations are also paid from left to right.
- 7 — match three to get 1000 coins.
- Watermelon — 3 are worth 500 coins.
- Three grape symbols worth 500 coins.
- 3-star symbols are worth 100 coins.
- 3-plum symbols are worth 200 coins.
- 3-lemon symbols worth 200 coins.
- 3-orange symbols worth 200 coins.
- 3-cherries are worth 200 coins.
Cash Connection Sizzling Hot Bonus Features by Novomatic
Lock 'N' Spin feature
This is a feature that lets you respin several times till you can get a winning combination. The feature can be triggered when 6 or more disc symbols are collected. Once, you attain this you will be awarded with 3 free spins. With the free spins, a player stands a chance to align 6 disc symbols again n trigger the feature again.
Even though this slot machine lacks wilds and free spins, users will enjoy the bonus scatter symbol. The main symbol in the slot is the star. Match 3, 4 and 5 stars to be awarded. You will earn a hundred points if you match 3 stars and 500 points for four stars. Five stars will earn you 2500 points.
Cash Connection Sizzling Hot Theme and Design
The slot features a fruity, classic theme and gets its name from the flames that show up when a player lands a winning a combo. Although, it spots a simple design it will still give you an awesome casino experience. The symbols include fruits such as watermelons, oranges, plums and grapes. Classic symbols such as fortunate seven and star are part of the symbols.
This slot has an interface full of bright colours. The red colour on slot's interface symbolizes the hotness as implied by its name. The developer has integrated both the retro and modern looks to give an elegant yet simple design. It has a purple background. However, there is not much to write about this slot when it comes to the sound.
Conclusion and Verdict
The fruit theme coupled with a lock 'n' spin feature is what appeals for this slot game. This is because a fruit theme offers familiarity for both experienced and new players hence guaranteeing an easy, yet fun gaming experience. The lock n spin feature offers a chance for several twists and thrills during gameplay for such a basic slot.
Additionally, the maximum payout of 1000x the stake plus a progressive jackpot is also a good deal. Added to this are a number of bonus features designed to keep the game flowing. At the end of the day, Cash Connection Sizzling Hot slot is a lucrative choice. Play now on Gentingbet and get a taste of some true fun.
Genting Casino is a leading global casino brand. We provide a premium online casino experience with our huge selection of online slots and live casino games. Genting has been recognized many times for its work in creating fun, safe gaming experiences winning multiple industry awards during its 50 years in business.

domain: gamble


text:
You get up every morning with a certain expectation of how the day will go. You leave for work knowing that there will be expectations when you arrive. Throughout the day, you will be interacting with scores of people who have defined expectations of you. Likewise, you have expectations of them. It all continues when you get home at the end of the day. It doesn’t cease until you climb into bed and fall asleep.
Expectations are a normal part of life. We all have them. We all try to live up to them. However, there are three hidden dangers of living this way. They are discussed in a post on the Manhattan Mental Health Counseling website. This post will discuss them in a little more detail.
1. They Are Not an Accurate Reflection
Expectations are a reflection of the person who projects them. They are what that person wants; what that person believes is right. The problem is that expectations are not an accurate reflection of the thoughts ideas of everyone in a group – even when that group has a shared identity, belief system, etc.
Your expectations of someone else probably do not reflect who that person is. Likewise, the expectations others have of you don’t necessarily reflect who you are. We all have different perspectives. We have different starting points from which we see the world and make decisions about it. Trying to meet someone else’s expectations requires trying to fit into their worldview. Projecting your expectations on someone else is projecting your worldview.
2. They Are Almost Always Unrealistic
The curious thing about expectations is that they are almost always unrealistic. They are based in an idealistic view of what life would be like if it were perfect. Your boss might have the expectation that you are going to complete a certain amount of work before the day is over. But that expectation probably doesn’t account for things going wrong.
Likewise, you may have certain expectations of your spouse. In your eyes, that other person should always behave a certain way. Going one step further, your spouse’s behavior should always please you. But such expectations are not realistic. Your spouse is a flawed human being, as are you.
Unrealistic expectations are often unmet for that very reason. If we are not careful, we can allow them to lead us to a place of anger and resentment. More than one relationship has been harmed by unrealistic expectations and the inability to meet them.
3. They Are Rarely Consistent
Manhattan Mental Health Counseling’s third point on this particular topic is that “expectations are contradictory.” Another way to say it is that they are inconsistent. Because no two people see the world exactly alike, no two people have exactly the same expectations of others. But it goes deeper than that. What you expect from one person is probably not the same thing you expect from another. Your expectations are consistent as are those projected on you by others.
Plurawl, a New York City LatinX clothing brand for Hispanic communities, is very big on the idea of authenticity. They encourage their customers to live authentic lives in celebration of their heritage and culture. One of their T-shirts even goes as far as to encourage them to not try to live up to others’ expectations.
That is not bad advice. Expectations are not an accurate reflection of those we project them on. They are almost always unrealistic and rarely consistent. So rather than obsessing over them, it is better to keep them in perspective and move on from there.

domain: psychology


text:
Australian artist James Reka aka Reka One has been on a whirlwind painting trip across Europe, hitting spots in Paris, Berlin,Milan, London and Bristol.
We last featured work from the artist back in August of this year when he painted this mural in Dalston, East London before embarking on his mini Euro tour. It would appear REKA is back in London as we spotted this freshly painted piece late yesterday evening on Rivington Street on our route home. If you are in London and want to check the wall in person you can find it in Cargo's courtyard opposite the Banksy piece.
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domain: painting